LOCUS Untitled_pEXPR_#2 5114 bp DNA circular 11/02/2019 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=GateWay(tm) LR reaction between pDONR223 LC3A WT as an Entry vector and pDEST-CMV 3xFLAG-gateway as a Destination vector . COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers primer_bind 2380..2397 /label=M13-fwd /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=M13-fwd - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 141..158 \ primer_bind complement(4730..4750) /label=M13-rev /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=M13-rev - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - complement(3777..3797) \ primer_bind complement(4700..4719) /label=T7 /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=T7 - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - complement(3747..3766) \ rep_origin complement(3..685) /label=ColE1 origin /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=ColE1 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - complement(4164..4846) \ rep_origin 1789..2095 /label=F1 ori /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=F1 ori - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - 5950..6256 \ CDS complement(2241..2309) /label=LacZ alpha /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=LacZ alpha - ApEinfo_fwdcolor=#6495ed - ApEinfo_revcolor=#6495ed - complement(2..70) \ misc_binding complement(4756..4778) /label=LacO /SerialCloner_Color=&h808000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=LacO - ApEinfo_fwdcolor=#6495ed - ApEinfo_revcolor=#6495ed - complement(3803..3825) \ CDS complement(783..1442) /label=AmpR /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=yellow - ApEinfo_revcolor=yellow - complement(4944..5603) \ promoter 2634..3160 /label=CMV promoter /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=CMV promoter - ApEinfo_fwdcolor=cornflower blue - ApEinfo_revcolor=cornflower blue - 395..921 \ promoter 4466..4662 /label=SV40 early promoter /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=SV40 early promoter - ApEinfo_fwdcolor=cornflower blue - ApEinfo_revcolor=cornflower blue - 3513..3709 \ polyA_signal 3682..4162 /label=hGH polyA signal /SerialCloner_Color=&hFFC0CB /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=hGH polyA signal - ApEinfo_fwdcolor=pink - ApEinfo_revcolor=pink - 2729..3209 \ misc_feature 3175..3243 /label=3xFLAG /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=3xflag - ApEinfo_fwdcolor=#00ff00 - ApEinfo_revcolor=green - 1002..1004 \ 3254..3656 /label=Insert from pDONR223 LC3A WT /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment inserted from pDONR223 LC3A WT into pDEST-CMV 3xFLAG-gateway [LR] reaction \ promoter complement(4701..4719) /label=T7 promoter /SerialCloner_Color=&hF08080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label="T7_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ promoter complement(4730..4748) /label=M13 reverse primer /SerialCloner_Color=&hF08080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ CDS 3274..3636 /label=LC3A /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 61 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 121 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 181 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT 241 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 301 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 361 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 421 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC 481 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 541 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 601 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 661 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 721 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 781 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 841 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 901 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 961 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 1021 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 1081 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 1141 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 1201 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 1261 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 1321 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 1381 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 1441 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 1501 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 1561 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 1621 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 1681 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 1741 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 1801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 1861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 1921 AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 1981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 2041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 2101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 2161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACAAAA 2221 TATTAACGTT TACAATTTCC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA 2281 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA 2341 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC 2401 AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA 2461 GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 2521 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT 2581 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA 2641 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 2701 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 2761 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 2821 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 2881 TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 2941 ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 3001 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 3061 CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 3121 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTGATCTGG TACCATGGAC 3181 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ATTACAAGGA TGACGATGAC 3241 AAGCTTACAA GTTTGTACAA AAAAGCAGGC TTAATGCCCT CAGACCGGCC TTTCAAGCAG 3301 CGGCGGAGCT TCGCCGACCG CTGTAAGGAG GTACAGCAGA TCCGCGACCA GCACCCCAGC 3361 AAAATCCCGG TGATCATCGA GCGCTACAAG GGTGAGAAGC AGCTGCCCGT CCTGGACAAG 3421 ACCAAGTTTT TGGTCCCGGA CCATGTCAAC ATGAGCGAGT TGGTCAAGAT CATCCGGCGC 3481 CGCCTGCAGC TGAACCCCAC GCAGGCCTTC TTCCTGCTGG TGAACCAGCA CAGCATGGTG 3541 AGTGTGTCCA CGCCCATCGC GGACATCTAC GAGCAGGAGA AAGACGAGGA CGGCTTCCTC 3601 TATATGGTCT ACGCCTCCCA GGAAACCTTC GGCTTCTAAT ACCCAGCTTT CTTGTACAAA 3661 GTGGTGTGAT CTAGAGGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT 3721 GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT 3781 CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG 3841 CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG 3901 GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT 3961 CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT 4021 TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA 4081 ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC 4141 TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC 4201 ACAGCATAGG CTACCTGCCA TGGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC 4261 TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC 4321 TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4381 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAAGTC CCCAGGCTCC 4441 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 4501 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 4561 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 4621 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCT CGAGGAACTG 4681 AAAAACCAGA AAGTTAATTC CCTATAGTGA GTCGTATTAA ATTCGTAATC ATGGTCATAG 4741 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 4801 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 4861 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 4921 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 4981 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 5041 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 5101 GCCAGGAACC GTAA //