LOCUS       pDEST-CMV_3xFLAG-LC3A-GFP    6511 bp      DNA     circular   11/02/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) LR reaction between pDONR223 LC3A no stop codon 
            as an Entry vector and pDEST-CMV-3xFLAG-gateway-EGFP as a De
            stination vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    319..606
                     /label=CAG_enhancer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="CAG_enhancer" - 315..602 \ 
     misc_feature    773..793
                     /label=CMV_fwd_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="CMV_fwd_primer" - 769..789 \ 
     promoter        complement(4413..4436)
                     /label=lac_promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="lac_promoter" - translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQS \ 
     misc_feature    complement(2106..2123)
                     /label=BGH_rev_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="BGH_rev_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRM \ 
     terminator      2109..2336
                     /label=bGH_PA_terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="bGH_PA_terminator" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHV \ 
     rep_origin      2399..2705
                     /label=f1_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="f1_origin" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQ \ 
     misc_feature    complement(2819..2839)
                     /label=pBABE_3_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBABE_3_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRM \ 
     misc_feature    complement(2825..3040)
                     /label=SV40_enhancer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="SV40_enhancer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRML \ 
     promoter        2837..3105
                     /label=SV40_promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="SV40_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRML \ 
     rep_origin      3004..3081
                     /label=SV40_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="SV40_origin" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ 
     misc_feature    3066..3085
                     /label=SV40pro_F_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="SV40pro_F_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVG \ 
     gene            3223..4011
                     /label=NeoR/KanR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="NeoR/KanR" - gene="NeoR/KanR" - translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR - PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL - LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE - EHQGLAPAELFARLKARMPDGEDLVVTHG \ 
     terminator      4191..4310
                     /label=SV40_PA_terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="SV40_PA_terminator" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - 5443..5562 \ 
     misc_feature    4279..4298
                     /label=EBV_rev_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="EBV_rev_primer" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - 5531..5550 \ 
     promoter        complement(4354..4372)
                     /label=M13_reverse_primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - complement(5606 \ 
     misc_feature    complement(4371..4393)
                     /label=M13_pUC_rev_primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_pUC_rev_primer" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - complement(5623 \ 
     promoter        complement(4407..4436)
                     /label=lac_promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="lac_promoter" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - complement(5659..5688 \ 
     gene            complement(5519..6379)
                     /label=Ampicillin
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="Ampicillin" - gene="Ampicillin" - translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA - VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA - VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ - PPHCISHDGYFLGRSKVR*" - co \ 
     promoter        complement(6421..6449)
                     /label=AmpR_promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="AmpR_promoter" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE - YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL \ 
     misc_feature    265..840
                     /label=CMV Promoter
                     /SerialCloner_Color=&h008080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4744..5364
                     /label=Ori
                     /SerialCloner_Color=&h000080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=note="direct strand translation"translation="PRCWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*" \ 
     CDS             1360..2079
                     /label=EGFP
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4354..4374)
                     /label=M13-rev
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13-rev - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - complement(3777..3797) \ 
     misc_binding    complement(4380..4402)
                     /label=LacO
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=LacO - ApEinfo_fwdcolor=#6495ed - ApEinfo_revcolor=#6495ed - complement(3803..3825) \ 
     CDS             complement(5522..6181)
                     /label=AmpR
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=yellow - ApEinfo_revcolor=yellow - complement(4944..5603) \ 
     misc_feature    856..924
                     /label=3xFLAG
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=3xflag - ApEinfo_fwdcolor=#00ff00 - ApEinfo_revcolor=green - 1002..1004 \ 
                     935..1334
                     /label=Insert from pDONR223 LC3A no stop codon
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from pDONR223 LC3A no stop codon into pDEST-CMV-3xFLAG-gateway-EGFP [LR] reaction \ 
     promoter        complement(4354..4372)
                     /label=M13 reverse primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ 
     CDS             955..1317
                     /label=LC3A
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTGCA CTCTCAGTAC AATCTGCTCT 
       61 GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG 
      121 TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT 
      181 CTGCTTAGGG TTAGGCGTTT TGCGCTGCTT CGCGATGTAC GGGCCAGATA TACGCGTTGA 
      241 CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA 
      301 TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC 
      361 GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT 
      421 TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA 
      481 GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG 
      541 CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA 
      601 GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG 
      661 TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG 
      721 CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG 
      781 GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGag ctCGTTTAGT GAACCGTCAG 
      841 AATTGATCTG gtacCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 
      901 GATTACAAGG ATGACGATGA CAAGCTTACA AGTTTGTACA AAAAAGCAGG CTTAATGCCC 
      961 TCAGACCGGC CTTTCAAGCA GCGGCGGAGC TTCGCCGACC GCTGTAAGGA GGTACAGCAG 
     1021 ATCCGCGACC AGCACCCCAG CAAAATCCCG GTGATCATCG AGCGCTACAA GGGTGAGAAG 
     1081 CAGCTGCCCG TCCTGGACAA GACCAAGTTT TTGGTCCCGG ACCATGTCAA CATGAGCGAG 
     1141 TTGGTCAAGA TCATCCGGCG CCGCCTGCAG CTGAACCCCA CGCAGGCCTT CTTCCTGCTG 
     1201 GTGAACCAGC ACAGCATGGT GAGTGTGTCC ACGCCCATCG CGGACATCTA CGAGCAGGAG 
     1261 AAAGACGAGG ACGGCTTCCT CTATATGGTC TACGCCTCCC AGGAAACCTT CGGCTTCTAC 
     1321 CCAGCTTTCT TGTACaaagt ggttcgatct agaCtcgaGA TGGTGAGCAA GGGCGAGGAG 
     1381 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 
     1441 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 
     1501 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 
     1561 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 
     1621 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 
     1681 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 
     1741 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 
     1801 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 
     1861 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 
     1921 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 
     1981 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 
     2041 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAT TAatTAAGAT AAACCCGCTG 
     2101 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 
     2161 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 
     2221 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 
     2281 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 
     2341 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 
     2401 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 
     2461 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 
     2521 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 
     2581 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 
     2641 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 
     2701 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 
     2761 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 
     2821 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     2881 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     2941 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     3001 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     3061 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 
     3121 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     3181 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     3241 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     3301 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     3361 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     3421 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     3481 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     3541 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     3601 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     3661 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     3721 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     3781 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     3841 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     3901 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     3961 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     4021 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     4081 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     4141 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     4201 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     4261 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     4321 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     4381 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     4441 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     4501 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     4561 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     4621 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     4681 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     4741 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     4801 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     4861 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     4921 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     4981 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     5041 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     5101 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     5161 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     5221 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     5281 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     5341 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     5401 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     5461 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     5521 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     5581 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     5641 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     5701 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     5761 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     5821 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     5881 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     5941 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     6001 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     6061 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     6121 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     6181 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     6241 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     6301 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     6361 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     6421 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     6481 CGCGCACATT TCCCCGAAAA GTGCCACCTG A
//