LOCUS       pET-His-Cre             6202 bp ds-DNA     circular     11-FEB-2019
DEFINITION  .
ACCESSION   urn.local...s-9lxvkhi
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /note="Geneious type: regulatory sequence"
     primer_bind     complement(34..94)
     gene            47..63
     misc_feature    71..94
     misc_feature    72..119
                     /note="Geneious type: overhang"
     primer_bind     95..133
     CDS             104..1135
     source          <104..>1129
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          complement(<104..>1129)
     primer_bind     complement(104..127)
     primer_bind     131..147
     primer_bind     600..625
     primer_bind     complement(600..625)
     misc_feature    1110..>1160
                     /note="Geneious type: overhang"
     primer_bind     complement(1111..1129)
     source          1128..>1129
     primer_bind     1130..1160
     terminator      1249..1292
     primer_bind     complement(1305..1329)
     misc_feature    1345..1651
                     /note="Geneious type: origin of replication"
     misc_feature    complement(1898..2758)
                     /note="Geneious type: marker"
     promoter        complement(2801..2829)
     misc_feature    2916..3535
                     /note="Geneious type: origin of replication"
     gene            complement(3950..4141)
     misc_feature    complement(4713..5804)
                     /note="Geneious type: regulatory sequence"
     primer_bind     6031..6054
     promoter        6186..2
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggcgcatc accaccacca tcacggcggg agcagcaatt tactgaccgt
      121 acaccaaaat ttgcctgcat tgccggtcga tgcaacgagt gatgaggttc gcaagaacct
      181 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
      241 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
      301 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
      361 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
      421 aagtgacagc aatgctgttt cactggttat gcggcgtatc cgaaaagaaa acgttgatgc
      481 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
      541 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
      601 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
      661 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
      721 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
      781 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
      841 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
      901 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
      961 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     1021 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     1081 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgact aatgactcga
     1141 gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag
     1201 cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1261 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
     1321 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1561 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1621 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1681 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1741 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttctggcgg cacgatggca
     1801 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1861 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1921 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1981 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2041 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2101 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2161 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2221 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2281 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2341 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2401 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2461 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2521 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2581 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2641 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2701 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2761 tcttcctttt tcaatcatga ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2821 atatttgaat gtatttagaa aaataaacaa ataggtcatg accaaaatcc cttaacgtga
     2881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3061 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3721 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc
     3781 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3841 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3901 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3961 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     4021 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     4081 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     4141 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     4201 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     4261 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     4321 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     4381 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     4441 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     4501 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     4561 agcctagccg ggtcctcaac gacaggagca cgatcatgct agtcatgccc cgcgcccacc
     4621 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     4681 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4801 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     4861 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     4921 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     4981 cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     5041 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     5101 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     5161 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     5221 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     5281 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     5341 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     5401 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     5461 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     5521 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     5581 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     5641 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     5701 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     5761 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     5821 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
     5881 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     5941 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     6001 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     6061 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     6121 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc
     6181 gaaattaata cgactcacta ta
//
