LOCUS       pET-His-VCre            6310 bp ds-DNA     circular     11-FEB-2019
DEFINITION  .
ACCESSION   urn.local...11-99offcm
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /note="Geneious type: regulatory sequence"
     gene            47..63
     misc_feature    71..94
     misc_feature    76..>103
                     /note="Geneious type: overhang"
     primer_bind     complement(79..103)
     CDS             104..1243
     misc_feature    105..1242
                     /note="Geneious type: Editing History Replacement"
     primer_bind     602..626
     primer_bind     complement(602..626)
     misc_feature    1217..1269
                     /note="Geneious type: overhang"
     primer_bind     complement(1217..1243)
     primer_bind     1244..1268
     terminator      1357..1400
     primer_bind     complement(1413..1437)
     misc_feature    1453..1759
                     /note="Geneious type: origin of replication"
     misc_feature    complement(2006..2866)
                     /note="Geneious type: marker"
     promoter        complement(2909..2937)
     misc_feature    3024..3643
                     /note="Geneious type: origin of replication"
     gene            complement(4058..4249)
     misc_feature    complement(4821..5912)
                     /note="Geneious type: regulatory sequence"
     primer_bind     6139..6162
     promoter        6294..2
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggcgcatc accaccacca tcacggcggg agcatagaga atcagctaag
      121 cttactgggc gatttttctg gagtacgccc tgacgatgta aaaacggcta tccaggctgc
      181 gcagaaaaaa ggaattaatg tcgccgaaaa tgaacagttt aaagccgcgt ttgagcacct
      241 gctgaatgag ttcaagaaac gcgaggaacg ttatagtcca aatactctca ggcggttaga
      301 gtcggcgtgg acttgctttg tggattggtg tcttgccaac catcgacatt ctctgccggc
      361 aactccagat acagtagaag cgttcttcat tgagagagca gaggagcttc atcgtaacac
      421 cttatcagtt tatcgttggg caatttcgcg ggtgcaccgc gtagccggtt gccctgatcc
      481 gtgtctagat atatatgtag aagatcgtct aaaggctata gctcggaaga aagtgcgcga
      541 aggtgaagct gtcaaacagg ctagtccctt taacgagcag caccttctga agctgactag
      601 tctgtggtat cgaagtgata aactcctgct taggcgcaat ttagcattgc tggccgtggc
      661 ttacgagtct atgttacggg catcggaact ggcgaacatc cgagtgtccg atatggaact
      721 tgctggagat ggtacagcga tccttaccat tcctattacg aaaacaaatc actccggtga
      781 acccgacaca tgtattctct cacaggacgt tgtctctctg ctgatggact atacggaggc
      841 aggcaaactc gatatgtcta gcgacggttt tctatttgtt ggggtcagta aacataatac
      901 gtgtatcaaa ccgaagaagg ataaacaaac aggcgaagtg cttcacaaac ctattacgac
      961 aaagacggta gaaggagtct tctattcggc atgggaaacc ttagacttgg gaaggcaagg
     1021 ggtgaaacct tttactgcac actctgcccg tgtgggagcc gcacaggatc tgctgaagaa
     1081 gggctataac accttacaga tccagcagag cgggagatgg agctctggcg caatggtggc
     1141 ccgttacggt cgtgctattc tggcccgaga tggcgccatg gcacattcta gagtaaaaac
     1201 acgttccgcg ccaatgcaat ggggcaaaga tgaaaaggac taactcgagt ctggtaaaga
     1261 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt
     1321 aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     1381 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
     1441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1861 aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa
     1921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2881 aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2941 atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca
     3001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3061 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3181 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3721 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3781 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3841 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     3901 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     3961 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4021 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4081 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     4141 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     4201 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     4261 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     4321 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     4381 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     4441 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     4501 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     4561 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     4621 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     4681 tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga
     4741 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     4801 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4861 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     4921 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     4981 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     5041 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     5101 atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     5161 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     5221 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     5281 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     5341 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     5401 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     5461 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     5521 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     5581 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     5641 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     5701 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     5761 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     5821 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     5881 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     5941 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     6001 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     6061 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     6121 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     6181 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     6241 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
     6301 actcactata
//
