LOCUS       Exported                4347 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  Ligation of pSB3K3* into product.
ACCESSION   .
VERSION     .
KEYWORDS    pXWJ109ParsR-gfp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        22..56
                     /label=J23109
     RBS             65..79
                     /label=B0030
     gene            86..439
                     /label=arsR
     terminator      494..622
                     /label=B0015
     promoter        631..732
                     /label=ParsR
     RBS             741..755
                     /label=B0030
     CDS             762..1478
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1490..1618
                     /label=B0015
     misc_feature    1619..1639
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(1857..2672)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(3267..3811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      4301..4344
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(4347,1..21)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aattcgcggc cgcttctaga gtttacagct agctcagtcc tagggactgt gctagctact
       61 agagattaaa gaggagaaat actagatgtc atttctgtta cccatccaat tgttcaaaat
      121 tcttgctgat gaaacccgtc tgggcatcgt tttactgctc agcgaactgg gagagttatg
      181 cgtctgcgat ctctgcactg ctctcgacca gtcgcagccc aagatctccc gccacctggc
      241 attgctgcgt gaaagcgggc tattgctgga ccgcaagcaa ggtaagtggg ttcattaccg
      301 cttatcaccg catattccag catgggcggc gaaaattatt gatgaggcct ggcgatgtga
      361 acaggaaaag gttcaggcga ttgtccgcaa cctggctcga caaaactgtt ccggggacag
      421 taagaacatt tgcagttaaa aatttagcta aacacacatg aattttcaga tgtgttttat
      481 ccgggtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      541 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      601 ggtgggcctt tctgcgttta tatactagag ccaactcaaa attcacacct attaccttcc
      661 tctgcactta cacattcgtt aagtcatata tgtttttgac ttatccgctt cgaagagaga
      721 cactacctgc aatactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac
      781 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      841 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      901 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      961 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     1021 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1081 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     1141 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     1201 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1261 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1321 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1381 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1441 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1501 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1561 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1621 ctagtagcgg ccgctgcagt ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt
     1681 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     1741 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatctcgag
     1801 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
     1861 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     1921 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     1981 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2041 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2101 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     2161 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2221 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2281 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2341 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     2401 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2461 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2521 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2581 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     2641 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     2701 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     2761 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     2821 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
     2881 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
     2941 gatggggcga ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag
     3001 cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc
     3061 ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat
     3121 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga
     3181 aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga
     3241 agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac
     3301 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     3361 tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     3421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     3481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     3541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     3601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     3661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     3721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     3781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg ggtctgacgc
     3841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4201 atccgcctcc atccagtcta ttccatggtg ccacctgacg tctaagaaac cattattatc
     4261 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     4321 ttagctttcg ctaaggatga tttctgg
//
