LOCUS       Exported                6629 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  Ligation of pSB3K3* into product.
ACCESSION   .
VERSION     .
KEYWORDS    pXWJ109ParsR-Amp30M-gfp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        22..56
                     /label=J23109
     RBS             65..79
                     /label=B0030
     gene            86..439
                     /label=arsR
     terminator      494..622
                     /label=B0015
     promoter        631..732
                     /label=ParsR
     RBS             741..755
                     /label=B0030
     gene            762..1709
                     /label=hrpR
     RBS             1737..1751
                     /label=B0030
     gene            1758..2669
                     /label=hrpS
     terminator      2670..2798
                     /label=B0015
     promoter        2807..3014
                     /label=PhrpLE
     RBS             3023..3037
                     /label=B0030
     CDS             3044..3760
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3772..3900
                     /label=B0015
     misc_feature    3901..3921
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(4139..4954)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(5549..6093)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      6583..6626
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(6629,1..21)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aattcgcggc cgcttctaga gtttacagct agctcagtcc tagggactgt gctagctact
       61 agagattaaa gaggagaaat actagatgtc atttctgtta cccatccaat tgttcaaaat
      121 tcttgctgat gaaacccgtc tgggcatcgt tttactgctc agcgaactgg gagagttatg
      181 cgtctgcgat ctctgcactg ctctcgacca gtcgcagccc aagatctccc gccacctggc
      241 attgctgcgt gaaagcgggc tattgctgga ccgcaagcaa ggtaagtggg ttcattaccg
      301 cttatcaccg catattccag catgggcggc gaaaattatt gatgaggcct ggcgatgtga
      361 acaggaaaag gttcaggcga ttgtccgcaa cctggctcga caaaactgtt ccggggacag
      421 taagaacatt tgcagttaaa aatttagcta aacacacatg aattttcaga tgtgttttat
      481 ccgggtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      541 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      601 ggtgggcctt tctgcgttta tatactagag ccaactcaaa attcacacct attaccttcc
      661 tctgcactta cacattcgtt aagtcatata tgtttttgac ttatccgctt cgaagagaga
      721 cactacctgc aatactagag attaaagagg agaaatacta gatgagtaca ggcatcgata
      781 aggacgtccg agagtgttgg ggcgtaactg cattatcagc gggtcatcaa attgcaatga
      841 atagcgcgtt tctggatatg gacttgctgt tgtgcgggga aaccggcacc ggcaaggaca
      901 cactggccaa ccgcattcac gagttgtcca gcaggtcggg accctttgtg ggcatgaact
      961 gcgccgccat tcccgagtcg ctggcagaga gccagttatt cggtgtggtc aacggtgcat
     1021 tcaccggcgt atgccgggct cgcgagggct acatagaggc ctccagtggt ggcaccttgt
     1081 acctggatga aatcgacagc atgccgttga gcctgcaagc caaactgctg cgtgtgttgg
     1141 agagtcgagg tatcgagcgt ctgggctcga ccgaatttat cccggtggat ctgcggatca
     1201 ttgcctcggc ccagcggcca ctggatgaac tggtggaaca aggacttttc cgtcgcgacc
     1261 tgttttttcg gctcaacgtg ctgacgcttc acttgccagc cttgcgcaaa cgtcgtgaac
     1321 agatcctgcc attgttcgac cagttcaccc agggtatcgc tgccgagttc ggacgtcccg
     1381 ctcctgcgct ggacagcggg cgtgtgcagc tgctgctcag ccacgactgg ccgggcaaca
     1441 tccgcgaatt gaagtctgcg gccaagcgct tcgtactcgg cttccccttg ctgggcgccg
     1501 accctgtgga agcgcttgac cctgccacgg ggctgcgcac gcaaatgcgc atcatcgaga
     1561 aaatgctcat ccaggatgcc ttgaagcggc acaggcacaa tttcgacgcg gtgcttcagg
     1621 agttggagtt gccaagacgc accctgtatc accgcatgaa ggaactggga gttgcagcgc
     1681 cgatcgctgc gacggccggg gtctaataaa acgcaggtgt ggttatgcta ctagagatta
     1741 aagaggagaa atactagatg agtcttgatg aaaggtttga ggatgatctg gacgaggagc
     1801 gggttccgaa tctggggata gttgccgaaa gtatttcgca actgggtatc gacgtgctgc
     1861 tatcgggtga gaccggcacg ggcaaagaca cgattgcccg acggattcat gagatgtcag
     1921 gccgcaaagg gcgcctggtg gcgatgaatt gcgcggccat tccggagtcc ctcgccgaga
     1981 gcgagttatt cggcgtggtc agcggtgcct acaccggcgc tgatcgctcc agagtcggtt
     2041 atgtcgaagc ggcgcagggc ggcacgctgt acctggatga gatcgatagc atgccgctga
     2101 gcctgcaagc caaattgctg agggtgctgg aaacccgagc gcttgaacgg ctgggttcga
     2161 cgtcgacgat caagctggat atctgcgtga tcgcctccgc ccaatgctcg ctggacgacg
     2221 ccgtcgagcg ggggcagttt cgtcgcgatc tgtattttcg cctgaacgtc ctgacactca
     2281 agcttcctcc gctacgtaac cagtctgatc gcatagttcc cctgttcaca cgttttacgg
     2341 ccgccgccgc gagggagctc ggtgttcccg ttcccgatgt ttgcccactg ctgcacaaag
     2401 tgctgctggg ccacgactgg cccggcaata tccgtgagct caaggcggca gccaaacgcc
     2461 atgtgctggg tttccccttg ctgggcgccg agccgcaggg cgaagagcac ttggcctgtg
     2521 ggctcaaatc gcaattgcga gtgatcgaaa aagccctgat tcaggagtcg ctcaagcgcc
     2581 acgacaattg tgtggattcg gtaagcctgg aactggacgt gccacgccgt acgctctatc
     2641 gacgcatcaa agaattgcag atctaataac caggcatcaa ataaaacgaa aggctcagtc
     2701 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2761 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggccg gattatgtcc
     2821 gctgagtggg tcacggtccc ggatcagttc ccttgcgaag ctgaccgatg tttttgtgcc
     2881 aaaagctgtt gtggcaaaaa acggtttgcg caaagttttg tattacaaag aatttcacat
     2941 tttaaaatat ctttataaat caatcagtta tttctatttt caagctggca cggttattgc
     3001 tatagggctt gtactactag agattaaaga ggagaaatac tagatgcgta aaggagaaga
     3061 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     3121 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     3181 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     3241 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     3301 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     3361 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     3421 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     3481 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     3541 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     3601 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     3661 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     3721 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     3781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3841 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     3901 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
     3961 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     4021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg
     4081 agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     4141 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     4201 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     4261 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     4321 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     4381 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
     4441 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     4501 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     4561 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     4621 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     4681 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     4741 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     4801 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     4861 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     4921 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     4981 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     5041 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     5101 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     5161 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     5221 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     5281 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     5341 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     5401 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     5461 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     5521 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     5581 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     5641 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     5701 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     5761 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     5821 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     5881 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     5941 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     6001 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     6061 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     6121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6481 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta
     6541 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     6601 ccttagcttt cgctaaggat gatttctgg
//
