LOCUS       Exported                7980 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(364..1314)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1362..1584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2076..2170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3252..3295
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3298..3319
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3320..3354
                     /label=J23109
     RBS             3363..3375
                     /label=B0032
     gene            3382..3816
                     /label=merR
     terminator      3825..3953
                     /label=B0015
     promoter        3962..4018
                     /label=PmerT
     RBS             4027..4041
                     /label=B0030
     gene            4048..4995
                     /label=hrpR
     RBS             5023..5037
                     /label=B0030
     gene            5044..5955
                     /label=hrpS
     terminator      5964..6092
                     /label=B0015
     promoter        6101..6308
                     /label=PhrpLE
     RBS             6317..6331
                     /label=B0030
     gene            6338..6757
                     /label=RinA_p80-alpha
     terminator      6769..6897
                     /label=B0015
     promoter        6906..7074
                     /label=PrinA_p80a
     RBS             7083..7097
                     /label=B0030
     CDS             7104..7820
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7832..7960
                     /label=B0015
     misc_feature    join(7961..7980,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt
      181 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      241 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
      301 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      361 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      421 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
      481 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      541 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      601 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      661 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
      721 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
      781 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
      841 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
      901 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
      961 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1021 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1081 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1141 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1201 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1261 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1321 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1381 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1441 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1501 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1561 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1621 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     1741 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     1801 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     1861 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     1921 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     1981 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2041 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     2101 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2161 aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt
     3181 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3241 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3301 ttcgcggccg cttctagagt ttacagctag ctcagtccta gggactgtgc tagctactag
     3361 agtcacacag gaaagtacta gatggaaaat aatttggaaa acctgaccat tggcgttttt
     3421 gccaaggcgg ccggggtcaa cgtggagaca atccgcttct atcagcgcaa gggcctgttg
     3481 cgggaaccgg acaagcctta cggcagcatc cgccgctatg gggaggcgga cgtggttcgg
     3541 gtgaaattcg tgaaatcggc acagcggctg gggttcagtc tggacgagat tgccgagctg
     3601 ttgcggctcg acgatggcac ccactgcgag gaggccagca gcctggccga acacaagctc
     3661 aaggacgtgc gcgagaagat ggccgacttg gcgcgcatgg aaaccgtgct gtctgaactc
     3721 gtgtgcgcct gccatgcacg aaaggggaat gtttcctgcc cgttgatcgc gtcactacag
     3781 ggcgaagcag gcctggcaag gtcagctatg ccttagtact agagccaggc atcaaataaa
     3841 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3901 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatggatcc
     3961 gttccatatc gcttgactac gtacatgagt acggaagtaa ggttacgcta tccaatccta
     4021 ctagagatta aagaggagaa atactagatg agtacaggca tcgataagga cgtccgagag
     4081 tgttggggcg taactgcatt atcagcgggt catcaaattg caatgaatag cgcgtttctg
     4141 gatatggact tgctgttgtg cggggaaacc ggcaccggca aggacacact ggccaaccgc
     4201 attcacgagt tgtccagcag gtcgggaccc tttgtgggca tgaactgcgc cgccattccc
     4261 gagtcgctgg cagagagcca gttattcggt gtggtcaacg gtgcattcac cggcgtatgc
     4321 cgggctcgcg agggctacat agaggcctcc agtggtggca ccttgtacct ggatgaaatc
     4381 gacagcatgc cgttgagcct gcaagccaaa ctgctgcgtg tgttggagag tcgaggtatc
     4441 gagcgtctgg gctcgaccga atttatcccg gtggatctgc ggatcattgc ctcggcccag
     4501 cggccactgg atgaactggt ggaacaagga cttttccgtc gcgacctgtt ttttcggctc
     4561 aacgtgctga cgcttcactt gccagccttg cgcaaacgtc gtgaacagat cctgccattg
     4621 ttcgaccagt tcacccaggg tatcgctgcc gagttcggac gtcccgctcc tgcgctggac
     4681 agcgggcgtg tgcagctgct gctcagccac gactggccgg gcaacatccg cgaattgaag
     4741 tctgcggcca agcgcttcgt actcggcttc cccttgctgg gcgccgaccc tgtggaagcg
     4801 cttgaccctg ccacggggct gcgcacgcaa atgcgcatca tcgagaaaat gctcatccag
     4861 gatgccttga agcggcacag gcacaatttc gacgcggtgc ttcaggagtt ggagttgcca
     4921 agacgcaccc tgtatcaccg catgaaggaa ctgggagttg cagcgccgat cgctgcgacg
     4981 gccggggtct aataaaacgc aggtgtggtt atgctactag agattaaaga ggagaaatac
     5041 tagatgagtc ttgatgaaag gtttgaggat gatctggacg aggagcgggt tccgaatctg
     5101 gggatagttg ccgaaagtat ttcgcaactg ggtatcgacg tgctgctatc gggtgagacc
     5161 ggcacgggca aagacacgat tgcccgacgg attcatgaga tgtcaggccg caaagggcgc
     5221 ctggtggcga tgaattgcgc ggccattccg gagtccctcg ccgagagcga gttattcggc
     5281 gtggtcagcg gtgcctacac cggcgctgat cgctccagag tcggttatgt cgaagcggcg
     5341 cagggcggca cgctgtacct ggatgagatc gatagcatgc cgctgagcct gcaagccaaa
     5401 ttgctgaggg tgctggaaac ccgagcgctt gaacggctgg gttcgacgtc gacgatcaag
     5461 ctggatatct gcgtgatcgc ctccgcccaa tgctcgctgg acgacgccgt cgagcggggg
     5521 cagtttcgtc gcgatctgta ttttcgcctg aacgtcctga cactcaagct tcctccgcta
     5581 cgtaaccagt ctgatcgcat agttcccctg ttcacacgtt ttacggccgc cgccgcgagg
     5641 gagctcggtg ttcccgttcc cgatgtttgc ccactgctgc acaaagtgct gctgggccac
     5701 gactggcccg gcaatatccg tgagctcaag gcggcagcca aacgccatgt gctgggtttc
     5761 cccttgctgg gcgccgagcc gcagggcgaa gagcacttgg cctgtgggct caaatcgcaa
     5821 ttgcgagtga tcgaaaaagc cctgattcag gagtcgctca agcgccacga caattgtgtg
     5881 gattcggtaa gcctggaact ggacgtgcca cgccgtacgc tctatcgacg catcaaagaa
     5941 ttgcagatct aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     6001 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     6061 ctcaccttcg ggtgggcctt tctgcgttta tatactagag gccggattat gtccgctgag
     6121 tgggtcacgg tcccggatca gttcccttgc gaagctgacc gatgtttttg tgccaaaagc
     6181 tgttgtggca aaaaacggtt tgcgcaaagt tttgtattac aaagaatttc acattttaaa
     6241 atatctttat aaatcaatca gttatttcta ttttcaagct ggcacggtta ttgctatagg
     6301 gcttgtacta ctagagatta aagaggagaa atactagatg actaaaaaga aatatggatt
     6361 aaaattatca acagttcgaa agttagaaga tgagttgtgt gattatccta attatcataa
     6421 gcaactcgaa gatttaagaa gtgaaataat gacaccatgg attccaacag atacaaatat
     6481 aggcggggag tttgtaccgt ctaatacatc gaaaacagaa atggcagtaa ctaattatct
     6541 ttgtagtata cgaagaggta aaatccttga gtttaagagc gctattgaac gtataatcaa
     6601 cacatcaagt aggaaagaac gcgaatttat tcaagagtat tattttaata aaaaggaatt
     6661 agtgaaagtt tgtgatgaca tacacatttc tgatagaact gctcatagaa tcaaaaggaa
     6721 aatcatatcc agattggcgg aagagttagg ggaagagtga tactagagcc aggcatcaaa
     6781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6841 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     6901 tagagaattg gcagtaaagt ggcagttttt gatacctaaa atgagatatt atgatagtgt
     6961 aggatattga ctatcttact gcgtttccct tatcgcaatt aggaataaag gatctatgtg
     7021 ggttggctga ttatagccaa tcctttttta attttaaaaa gcgtatagcg cgagtactag
     7081 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
     7141 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     7201 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     7261 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     7321 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     7381 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     7441 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     7501 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     7561 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     7621 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     7681 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     7741 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     7801 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     7861 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     7921 cacactggct caccttcggg tgggcctttc tgcgtttata tactagtagc ggccgctgca
//
