LOCUS       Exported                6739 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(219..1034)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1629..2173)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      2663..2706
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2709..2730
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2731..2765
                     /label=J23109
     RBS             2774..2788
                     /label=B0030
     gene            2795..3148
                     /label=arsR
     terminator      3203..3331
                     /label=B0015
     promoter        3340..3441
                     /label=ParsR
     RBS             3450..3464
                     /label=B0030
     gene            3471..4418
                     /label=hrpR
     RBS             4446..4460
                     /label=B0030
     gene            4467..5378
                     /label=hrpS
     terminator      5387..5515
                     /label=B0015
     promoter        5524..5731
                     /label=PhrpLE
     RBS             5740..5754
                     /label=B0030
     CDS             5761..6474
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     mat_peptide     6520..6537
                     /label=TEV cleavage site
                     /label=TEV site
     mat_peptide     6544..6582
                     /label=AAV
     terminator      6591..6719
                     /label=B0015
     misc_feature    join(6720..6739,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg agtcccgtca agtcagcgta
      181 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      241 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      301 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      361 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      421 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      481 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      541 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      601 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      661 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      721 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      781 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      841 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      901 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      961 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1021 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1081 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1141 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1201 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1261 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1321 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     1381 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     1441 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     1501 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     1561 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     1621 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     1681 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc
     1741 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1801 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1861 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1981 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     2041 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     2101 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     2161 aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2581 tattccatgg tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2641 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2701 gatttctgga attcgcggcc gcttctagag tttacagcta gctcagtcct agggactgtg
     2761 ctagctacta gagattaaag aggagaaata ctagatgtca tttctgttac ccatccaatt
     2821 gttcaaaatt cttgctgatg aaacccgtct gggcatcgtt ttactgctca gcgaactggg
     2881 agagttatgc gtctgcgatc tctgcactgc tctcgaccag tcgcagccca agatctcccg
     2941 ccacctggca ttgctgcgtg aaagcgggct attgctggac cgcaagcaag gtaagtgggt
     3001 tcattaccgc ttatcaccgc atattccagc atgggcggcg aaaattattg atgaggcctg
     3061 gcgatgtgaa caggaaaagg ttcaggcgat tgtccgcaac ctggctcgac aaaactgttc
     3121 cggggacagt aagaacattt gcagttaaaa atttagctaa acacacatga attttcagat
     3181 gtgttttatc cgggtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     3241 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     3301 tcaccttcgg gtgggccttt ctgcgtttat atactagagc caactcaaaa ttcacaccta
     3361 ttaccttcct ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc
     3421 gaagagagac actacctgca atactagaga ttaaagagga gaaatactag atgagtacag
     3481 gcatcgataa ggacgtccga gagtgttggg gcgtaactgc attatcagcg ggtcatcaaa
     3541 ttgcaatgaa tagcgcgttt ctggatatgg acttgctgtt gtgcggggaa accggcaccg
     3601 gcaaggacac actggccaac cgcattcacg agttgtccag caggtcggga ccctttgtgg
     3661 gcatgaactg cgccgccatt cccgagtcgc tggcagagag ccagttattc ggtgtggtca
     3721 acggtgcatt caccggcgta tgccgggctc gcgagggcta catagaggcc tccagtggtg
     3781 gcaccttgta cctggatgaa atcgacagca tgccgttgag cctgcaagcc aaactgctgc
     3841 gtgtgttgga gagtcgaggt atcgagcgtc tgggctcgac cgaatttatc ccggtggatc
     3901 tgcggatcat tgcctcggcc cagcggccac tggatgaact ggtggaacaa ggacttttcc
     3961 gtcgcgacct gttttttcgg ctcaacgtgc tgacgcttca cttgccagcc ttgcgcaaac
     4021 gtcgtgaaca gatcctgcca ttgttcgacc agttcaccca gggtatcgct gccgagttcg
     4081 gacgtcccgc tcctgcgctg gacagcgggc gtgtgcagct gctgctcagc cacgactggc
     4141 cgggcaacat ccgcgaattg aagtctgcgg ccaagcgctt cgtactcggc ttccccttgc
     4201 tgggcgccga ccctgtggaa gcgcttgacc ctgccacggg gctgcgcacg caaatgcgca
     4261 tcatcgagaa aatgctcatc caggatgcct tgaagcggca caggcacaat ttcgacgcgg
     4321 tgcttcagga gttggagttg ccaagacgca ccctgtatca ccgcatgaag gaactgggag
     4381 ttgcagcgcc gatcgctgcg acggccgggg tctaataaaa cgcaggtgtg gttatgctac
     4441 tagagattaa agaggagaaa tactagatga gtcttgatga aaggtttgag gatgatctgg
     4501 acgaggagcg ggttccgaat ctggggatag ttgccgaaag tatttcgcaa ctgggtatcg
     4561 acgtgctgct atcgggtgag accggcacgg gcaaagacac gattgcccga cggattcatg
     4621 agatgtcagg ccgcaaaggg cgcctggtgg cgatgaattg cgcggccatt ccggagtccc
     4681 tcgccgagag cgagttattc ggcgtggtca gcggtgccta caccggcgct gatcgctcca
     4741 gagtcggtta tgtcgaagcg gcgcagggcg gcacgctgta cctggatgag atcgatagca
     4801 tgccgctgag cctgcaagcc aaattgctga gggtgctgga aacccgagcg cttgaacggc
     4861 tgggttcgac gtcgacgatc aagctggata tctgcgtgat cgcctccgcc caatgctcgc
     4921 tggacgacgc cgtcgagcgg gggcagtttc gtcgcgatct gtattttcgc ctgaacgtcc
     4981 tgacactcaa gcttcctccg ctacgtaacc agtctgatcg catagttccc ctgttcacac
     5041 gttttacggc cgccgccgcg agggagctcg gtgttcccgt tcccgatgtt tgcccactgc
     5101 tgcacaaagt gctgctgggc cacgactggc ccggcaatat ccgtgagctc aaggcggcag
     5161 ccaaacgcca tgtgctgggt ttccccttgc tgggcgccga gccgcagggc gaagagcact
     5221 tggcctgtgg gctcaaatcg caattgcgag tgatcgaaaa agccctgatt caggagtcgc
     5281 tcaagcgcca cgacaattgt gtggattcgg taagcctgga actggacgtg ccacgccgta
     5341 cgctctatcg acgcatcaaa gaattgcaga tctaataata ctagagccag gcatcaaata
     5401 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5461 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     5521 gaggccggat tatgtccgct gagtgggtca cggtcccgga tcagttccct tgcgaagctg
     5581 accgatgttt ttgtgccaaa agctgttgtg gcaaaaaacg gtttgcgcaa agttttgtat
     5641 tacaaagaat ttcacatttt aaaatatctt tataaatcaa tcagttattt ctattttcaa
     5701 gctggcacgg ttattgctat agggcttgta ctactagaga ttaaagagga gaaatactag
     5761 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     5821 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     5881 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     5941 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     6001 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     6061 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     6121 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     6181 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     6241 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     6301 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     6361 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     6421 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggcggc
     6481 tccggcggct ccggcggcac cggcggctcc ggcggctccg aaaacctgta ttttcagggt
     6541 ggcgctgcaa acgacgaaaa ctacgctgcc gcagtttaat aatactagag ccaggcatca
     6601 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     6661 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     6721 actagtagcg gccgctgca
//
