LOCUS       Exported                6359 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(364..1314)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1362..1584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2076..2170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3252..3295
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3298..3319
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3320..3462
                     /label=ParsR-ABS62
     RBS             3471..3483
                     /label=B0032
     gene            3490..4071
                     /label=ECF11_897
     terminator      4086..4214
                     /label=B0015
     promoter        4223..4282
                     /label=P11_3726
     RBS             4291..4303
                     /label=B0032
     gene            4310..5419
                     /label=malE
     mat_peptide     5465..5482
                     /label=TEV cleavage site
                     /label=TEV site
     gene            5489..6202
                     /label=TEV (S219V)
                     /label=TEV
     terminator      6211..6339
                     /label=B0015
     misc_feature    join(6340..6359,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt
      181 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      241 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
      301 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      361 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      421 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
      481 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      541 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      601 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      661 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
      721 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
      781 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
      841 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
      901 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
      961 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1021 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1081 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1141 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1201 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1261 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1321 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1381 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1441 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1501 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1561 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1621 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     1741 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     1801 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     1861 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     1921 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     1981 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2041 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     2101 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2161 aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt
     3181 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3241 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3301 ttcgcggccg cttctagagc caactcaaaa ttcacaccta ttaccttcct ctgcacttac
     3361 acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac actacctgca
     3421 aacttacaca taagtcatat atgtttttga cttatccgct tctactagag tcacacagga
     3481 aagtactaga tgatgagcga tagtccgcag aaactgggtc gtaatgaatg gaatgcatat
     3541 atggataaag tgaaagccaa agatcgtgaa gcctttgcct ttgtgtttcg tttttatgca
     3601 ccgaaactga aacagttcgc ctataaacat gttggcaatg aacaggttgc aatggaaatg
     3661 gttcaagaaa ccatggcaac cgtttggcag aaagcacatc tgtatgatgg taaaaaaagc
     3721 gcactgagca cctggattta taccattatt cgtaacctgt gctttgacct gctgcgtaaa
     3781 cagaaaggta aagaactgca tattcacagc gacgatattt ggccgagcga atattatccg
     3841 cctgatatgg ttgatcatta tagtccggaa caggatatgc tgaaagaaca ggtggttaaa
     3901 tttctggata tcctgccgaa aaatcagcgt gatgttctgc aagcagttta tctggaagaa
     3961 ctgccgcatc agcaggttgc agaactgttt gatattccgc tgggcaccgt taaaagccgt
     4021 ctgcgtctgg cagttgaaaa actgcgtcat agcatgcata ccgaacagct gtaataatac
     4081 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     4141 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     4201 tttctgcgtt tatatactag aggcctccac accgctcgtc acatcctgtg atccactctt
     4261 catcccgcta cgtaacacct cttactagag tcacacagga aagtactaga tgatcgaaga
     4321 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
     4381 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
     4441 agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca
     4501 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
     4561 gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat
     4621 tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa
     4681 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
     4741 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
     4801 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
     4861 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
     4921 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
     4981 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
     5041 aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc
     5101 aggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct
     5161 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
     5221 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc
     5281 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg
     5341 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
     5401 gcagactcgt atcaccaagg gcggctccgg cggctccggc ggcaccggcg gctccggcgg
     5461 ctccgaaaac ctgtattttc agggtggcgg cgaaagcctg ttcaaaggtc cgcgtgacta
     5521 caacccgatt agctcgacga tctgccacct gacgaacgaa agcgacggcc acaccacgag
     5581 cctgtatggc atcggttttg gcccgttcat tatcacgaac aaacacctgt ttcgtcgcaa
     5641 caatggtacc ctgctggtgc agtctctgca tggcgtgttt aaagttaaaa ataccacgac
     5701 cctgcaacaa cacctgatcg atggtcgtga catgattatc attcgcatgc cgaaagattt
     5761 tccgccgttc ccgcagaaac tgaaattccg tgaaccgcaa cgtgaagaac gcatttgcct
     5821 ggtcacgacc aactttcaga ccaaatcaat gagctctatg gttagcgata cgtcttgtac
     5881 ctttccgagt tccgacggca tcttctggaa acattggatt cagaccaaag atggtcaatg
     5941 cggcagtccg ctggtttcca cccgtgacgg tttcatcgtc ggcattcact cagcgtcgaa
     6001 ctttacgaat accaacaatt acttcacgtc cgttccgaaa aactttatgg aactgctgac
     6061 caatcaggaa gcgcagcaat gggtgtcagg ttggcgcctg aatgccgatt cggttctgtg
     6121 gggcggtcac aaagtcttta tggtgaaacc ggaagaaccg ttccagccgg tcaaagaagc
     6181 aacccaactg atgaattaat aatactagag ccaggcatca aataaaacga aaggctcagt
     6241 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     6301 acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg gccgctgca
//
