LOCUS       Exported                8038 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(364..1314)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1362..1584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2076..2170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3252..3295
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3298..3319
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3320..3354
                     /label=J23109
     RBS             3363..3377
                     /label=B0030
     gene            3384..3737
                     /label=arsR
     terminator      3792..3920
                     /label=B0015
     promoter        3929..4030
                     /label=ParsR
     protein_bind    3978..4001
                     /label=abs
                     /bound_moiety="ArsR"
     -35_signal      3994..3999
                     /label=-35
     -10_signal      4017..4022
                     /label=-10
     misc_feature    4031..4076
                     /label=ParsR (partial)
     protein_bind    4045..4068
                     /label=abs
                     /bound_moiety="ArsR"
     enhancer        4049..4053
                     /label=abs1?
     enhancer        4058..4063
                     /label=abs2?
     -35_signal      4061..4066
                     /label=-35
     RBS             4085..4099
                     /label=B0030
     gene            4106..5053
                     /label=hrpR
     RBS             5081..5095
                     /label=B0030
     gene            5102..6013
                     /label=hrpS
     terminator      6022..6150
                     /label=B0015
     promoter        6159..6366
                     /label=mPhrpL
     RBS             6375..6389
                     /label=B0030
     gene            6396..6815
                     /label=RinA_p80-alpha
     terminator      6827..6955
                     /label=B0015
     promoter        6964..7132
                     /label=PrinA_p80a
     RBS             7141..7155
                     /label=B0030
     gene            7162..7878
                     /label=gfp
     terminator      7890..8018
                     /label=B0015
     misc_feature    join(8019..8038,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt
      181 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      241 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
      301 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      361 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      421 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
      481 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      541 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      601 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      661 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
      721 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
      781 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
      841 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
      901 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
      961 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1021 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1081 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1141 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1201 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1261 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1321 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1381 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1441 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1501 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1561 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1621 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     1741 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     1801 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     1861 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     1921 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     1981 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2041 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     2101 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2161 aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt
     3181 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3241 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3301 ttcgcggccg cttctagagt ttacagctag ctcagtccta gggactgtgc tagctactag
     3361 agattaaaga ggagaaatac tagatgtcat ttctgttacc catccaattg ttcaaaattc
     3421 ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgaactggga gagttatgcg
     3481 tctgcgatct ctgcactgct ctcgaccagt cgcagcccaa gatctcccgc cacctggcat
     3541 tgctgcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt cattaccgct
     3601 tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg cgatgtgaac
     3661 aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc ggggacagta
     3721 agaacatttg cagttaaaaa tttagctaaa cacacatgaa ttttcagatg tgttttatcc
     3781 gggtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     3901 tgggcctttc tgcgtttata tactagagcc aactcaaaat tcacacctat taccttcctc
     3961 tgcacttaca cattcgttaa gtcatatatg tttttgactt atccgcttcg aagagagaca
     4021 ctacctgcaa acttacacat tcgttaagtc atatatgttt ttgacttatc cgcttctact
     4081 agagattaaa gaggagaaat actagatgag tacaggcatc gataaggacg tccgagagtg
     4141 ttggggcgta actgcattat cagcgggtca tcaaattgca atgaatagcg cgtttctgga
     4201 tatggacttg ctgttgtgcg gggaaaccgg caccggcaag gacacactgg ccaaccgcat
     4261 tcacgagttg tccagcaggt cgggaccctt tgtgggcatg aactgcgccg ccattcccga
     4321 gtcgctggca gagagccagt tattcggtgt ggtcaacggt gcattcaccg gcgtatgccg
     4381 ggctcgcgag ggctacatag aggcctccag tggtggcacc ttgtacctgg atgaaatcga
     4441 cagcatgccg ttgagcctgc aagccaaact gctgcgtgtg ttggagagtc gaggtatcga
     4501 gcgtctgggc tcgaccgaat ttatcccggt ggatctgcgg atcattgcct cggcccagcg
     4561 gccactggat gaactggtgg aacaaggact tttccgtcgc gacctgtttt ttcggctcaa
     4621 cgtgctgacg cttcacttgc cagccttgcg caaacgtcgt gaacagatcc tgccattgtt
     4681 cgaccagttc acccagggta tcgctgccga gttcggacgt cccgctcctg cgctggacag
     4741 cgggcgtgtg cagctgctgc tcagccacga ctggccgggc aacatccgcg aattgaagtc
     4801 tgcggccaag cgcttcgtac tcggcttccc cttgctgggc gccgaccctg tggaagcgct
     4861 tgaccctgcc acggggctgc gcacgcaaat gcgcatcatc gagaaaatgc tcatccagga
     4921 tgccttgaag cggcacaggc acaatttcga cgcggtgctt caggagttgg agttgccaag
     4981 acgcaccctg tatcaccgca tgaaggaact gggagttgca gcgccgatcg ctgcgacggc
     5041 cggggtctaa taaaacgcag gtgtggttat gctactagag attaaagagg agaaatacta
     5101 gatgagtctt gatgaaaggt ttgaggatga tctggacgag gagcgggttc cgaatctggg
     5161 gatagttgcc gaaagtattt cgcaactggg tatcgacgtg ctgctatcgg gtgagaccgg
     5221 cacgggcaaa gacacgattg cccgacggat tcatgagatg tcaggccgca aagggcgcct
     5281 ggtggcgatg aattgcgcgg ccattccgga gtccctcgcc gagagcgagt tattcggcgt
     5341 ggtcagcggt gcctacaccg gcgctgatcg ctccagagtc ggttatgtcg aagcggcgca
     5401 gggcggcacg ctgtacctgg atgagatcga tagcatgccg ctgagcctgc aagccaaatt
     5461 gctgagggtg ctggaaaccc gagcgcttga acggctgggt tcgacgtcga cgatcaagct
     5521 ggatatctgc gtgatcgcct ccgcccaatg ctcgctggac gacgccgtcg agcgggggca
     5581 gtttcgtcgc gatctgtatt ttcgcctgaa cgtcctgaca ctcaagcttc ctccgctacg
     5641 taaccagtct gatcgcatag ttcccctgtt cacacgtttt acggccgccg ccgcgaggga
     5701 gctcggtgtt cccgttcccg atgtttgccc actgctgcac aaagtgctgc tgggccacga
     5761 ctggcccggc aatatccgtg agctcaaggc ggcagccaaa cgccatgtgc tgggtttccc
     5821 cttgctgggc gccgagccgc agggcgaaga gcacttggcc tgtgggctca aatcgcaatt
     5881 gcgagtgatc gaaaaagccc tgattcagga gtcgctcaag cgccacgaca attgtgtgga
     5941 ttcggtaagc ctggaactgg acgtgccacg ccgtacgctc tatcgacgca tcaaagaatt
     6001 gcagatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     6061 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     6121 caccttcggg tgggcctttc tgcgtttata tactagaggc cggattatgt ccgctgagtg
     6181 ggtcacggtc ccggatcagt tcccttgcga agctgaccga tgtttttgtg ccaaaagctg
     6241 ttgtggcaaa aaacggtttg cgcaaagttt tgtattacaa agaatttcac attttaaaat
     6301 atctttataa atcaatcagt tatttctatt ttcaagctgg cacggttatt gctatagggc
     6361 ttgtactact agagattaaa gaggagaaat actagatgac taaaaagaaa tatggattaa
     6421 aattatcaac agttcgaaag ttagaagatg agttgtgtga ttatcctaat tatcataagc
     6481 aactcgaaga tttaagaagt gaaataatga caccatggat tccaacagat acaaatatag
     6541 gcggggagtt tgtaccgtct aatacatcga aaacagaaat ggcagtaact aattatcttt
     6601 gtagtatacg aagaggtaaa atccttgagt ttaagagcgc tattgaacgt ataatcaaca
     6661 catcaagtag gaaagaacgc gaatttattc aagagtatta ttttaataaa aaggaattag
     6721 tgaaagtttg tgatgacata cacatttctg atagaactgc tcatagaatc aaaaggaaaa
     6781 tcatatccag attggcggaa gagttagggg aagagtgata ctagagccag gcatcaaata
     6841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     6961 gagaattggc agtaaagtgg cagtttttga tacctaaaat gagatattat gatagtgtag
     7021 gatattgact atcttactgc gtttccctta tcgcaattag gaataaagga tctatgtggg
     7081 ttggctgatt atagccaatc cttttttaat tttaaaaagc gtatagcgcg agtactagag
     7141 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     7201 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     7261 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     7321 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     7381 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     7441 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     7501 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     7561 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     7621 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     7681 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     7741 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     7801 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     7861 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     7921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     7981 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagtagcgg ccgctgca
//
