LOCUS       Exported                7229 bp ds-DNA     circular SYN 11-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 11, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(364..1314)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1362..1584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2076..2170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3252..3295
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3298..3319
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3320..3354
                     /label=J23117
     RBS             3363..3377
                     /label=B0030
     gene            3384..3737
                     /label=arsR
     terminator      3792..3920
                     /label=B0015
     promoter        3929..4030
                     /label=ParsR
     RBS             4039..4053
                     /label=B0030
     gene            4060..5007
                     /label=hrpR
     RBS             5035..5049
                     /label=B0030
     gene            5059..5970
                     /label=hrpS
     terminator      5979..6107
                     /label=B0015
     promoter        6116..6323
                     /label=mPhrpL
     RBS             6332..6346
                     /label=B0030
     gene            6353..7069
                     /label=gfp
     terminator      7081..7209
                     /label=B0015
     misc_feature    join(7210..7229,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt
      181 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      241 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
      301 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      361 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      421 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
      481 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      541 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      601 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      661 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
      721 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
      781 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
      841 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
      901 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
      961 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1021 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1081 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1141 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1201 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1261 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1321 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1381 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1441 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1501 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1561 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1621 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     1741 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     1801 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     1861 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     1921 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     1981 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2041 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     2101 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2161 aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt
     3181 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3241 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3301 ttcgcggccg cttctagagt tgacagctag ctcagtccta gggattgtgc tagctactag
     3361 agattaaaga ggagaaatac tagatgtcat ttctgttacc catccaattg ttcaaaattc
     3421 ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgaactggga gagttatgcg
     3481 tctgcgatct ctgcactgct ctcgaccagt cgcagcccaa gatctcccgc cacctggcat
     3541 tgctgcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt cattaccgct
     3601 tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg cgatgtgaac
     3661 aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc ggggacagta
     3721 agaacatttg cagttaaaaa tttagctaaa cacacatgaa ttttcagatg tgttttatcc
     3781 gggtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     3901 tgggcctttc tgcgtttata tactagagcc aactcaaaat tcacacctat taccttcctc
     3961 tgcacttaca cattcgttaa gtcatatatg tttttgactt atccgcttcg aagagagaca
     4021 ctacctgcaa tactagagat taaagaggag aaatactaga tgagtacagg catcgataag
     4081 gacgtccgag agtgttgggg cgtaactgca ttatcagcgg gtcatcaaat tgcaatgaat
     4141 agcgcgtttc tggatatgga cttgctgttg tgcggggaaa ccggcaccgg caaggacaca
     4201 ctggccaacc gcattcacga gttgtccagc aggtcgggac cctttgtggg catgaactgc
     4261 gccgccattc ccgagtcgct ggcagagagc cagttattcg gtgtggtcaa cggtgcattc
     4321 accggcgtat gccgggctcg cgagggctac atagaggcct ccagtggtgg caccttgtac
     4381 ctggatgaaa tcgacagcat gccgttgagc ctgcaagcca aactgctgcg tgtgttggag
     4441 agtcgaggta tcgagcgtct gggctcgacc gaatttatcc cggtggatct gcggatcatt
     4501 gcctcggccc agcggccact ggatgaactg gtggaacaag gacttttccg tcgcgacctg
     4561 ttttttcggc tcaacgtgct gacgcttcac ttgccagcct tgcgcaaacg tcgtgaacag
     4621 atcctgccat tgttcgacca gttcacccag ggtatcgctg ccgagttcgg acgtcccgct
     4681 cctgcgctgg acagcgggcg tgtgcagctg ctgctcagcc acgactggcc gggcaacatc
     4741 cgcgaattga agtctgcggc caagcgcttc gtactcggct tccccttgct gggcgccgac
     4801 cctgtggaag cgcttgaccc tgccacgggg ctgcgcacgc aaatgcgcat catcgagaaa
     4861 atgctcatcc aggatgcctt gaagcggcac aggcacaatt tcgacgcggt gcttcaggag
     4921 ttggagttgc caagacgcac cctgtatcac cgcatgaagg aactgggagt tgcagcgccg
     4981 atcgctgcga cggccggggt ctaataaaac gcaggtgtgg ttatgctact agagattaaa
     5041 gaggagaaat actagatgat gagtcttgat gaaaggtttg aggatgatct ggacgaggag
     5101 cgggttccga atctggggat agttgccgaa agtatttcgc aactgggtat cgacgtgctg
     5161 ctatcgggtg agaccggcac gggcaaagac acgattgccc gacggattca tgagatgtca
     5221 ggccgcaaag ggcgcctggt ggcgatgaat tgcgcggcca ttccggagtc cctcgccgag
     5281 agcgagttat tcggcgtggt cagcggtgcc tacaccggcg ctgatcgctc cagagtcggt
     5341 tatgtcgaag cggcgcaggg cggcacgctg tacctggatg agatcgatag catgccgctg
     5401 agcctgcaag ccaaattgct gagggtgctg gaaacccgag cgcttgaacg gctgggttcg
     5461 acgtcgacga tcaagctgga tatctgcgtg atcgcctccg cccaatgctc gctggacgac
     5521 gccgtcgagc gggggcagtt tcgtcgcgat ctgtattttc gcctgaacgt cctgacactc
     5581 aagcttcctc cgctacgtaa ccagtctgat cgcatagttc ccctgttcac acgttttacg
     5641 gccgccgccg cgagggagct cggtgttccc gttcccgatg tttgcccact gctgcacaaa
     5701 gtgctgctgg gccacgactg gcccggcaat atccgtgagc tcaaggcggc agccaaacgc
     5761 catgtgctgg gtttcccctt gctgggcgcc gagccgcagg gcgaagagca cttggcctgt
     5821 gggctcaaat cgcaattgcg agtgatcgaa aaagccctga ttcaggagtc gctcaagcgc
     5881 cacgacaatt gtgtggattc ggtaagcctg gaactggacg tgccacgccg tacgctctat
     5941 cgacgcatca aagaattgca gatctaataa tactagagcc aggcatcaaa taaaacgaaa
     6001 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     6061 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggccgg
     6121 attatgtccg ctgagtgggt cacggtcccg gatcagttcc cttgcgaagc tgaccgatgt
     6181 ttttgtgcca aaagctgttg tggcaaaaaa cggtttgcgc aaagttttgt attacaaaga
     6241 atttcacatt ttaaaatatc tttataaatc aatcagttat ttctattttc aagctggcac
     6301 ggttattgct atagggcttg tactactaga gattaaagag gagaaatact agatgcgtaa
     6361 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     6421 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     6481 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     6541 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
     6601 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     6661 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     6721 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     6781 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     6841 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     6901 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     6961 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     7021 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
     7081 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     7141 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     7201 gcgtttatat actagtagcg gccgctgca
//
