LOCUS       Exported                7292 bp ds-DNA     circular SYN 12-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 12, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(364..1314)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1362..1584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2076..2170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3252..3295
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3298..3319
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3320..3354
                     /label=J23109
     RBS             3363..3377
                     /label=B0030
     gene            3384..3737
                     /label=arsR
     terminator      3792..3920
                     /label=B0015
     promoter        3929..4093
                     /label=ParsR-ABS84
     RBS             4102..4116
                     /label=B0030
     gene            4123..5070
                     /label=hrpR
     RBS             5098..5112
                     /label=B0030
     gene            5122..6033
                     /label=hrpS
     terminator      6042..6170
                     /label=B0015
     promoter        6179..6386
                     /label=mPhrpL
     RBS             6395..6409
                     /label=B0030
     CDS             6416..7132
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7144..7272
                     /label=B0015
     misc_feature    join(7273..7292,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt
      181 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      241 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
      301 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      361 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      421 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
      481 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      541 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      601 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      661 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
      721 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
      781 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
      841 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
      901 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
      961 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1021 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1081 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1141 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1201 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1261 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1321 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1381 acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1441 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1501 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1561 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1621 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     1741 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     1801 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     1861 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     1921 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     1981 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2041 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     2101 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2161 aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt
     3181 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3241 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3301 ttcgcggccg cttctagagt ttacagctag ctcagtccta gggactgtgc tagctactag
     3361 agattaaaga ggagaaatac tagatgtcat ttctgttacc catccaattg ttcaaaattc
     3421 ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgaactggga gagttatgcg
     3481 tctgcgatct ctgcactgct ctcgaccagt cgcagcccaa gatctcccgc cacctggcat
     3541 tgctgcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt cattaccgct
     3601 tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg cgatgtgaac
     3661 aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc ggggacagta
     3721 agaacatttg cagttaaaaa tttagctaaa cacacatgaa ttttcagatg tgttttatcc
     3781 gggtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     3901 tgggcctttc tgcgtttata tactagagcc aactcaaaat tcacacctat taccttcctc
     3961 tgcacttaca cattcgttaa gtcatatatg tttttgactt atccgcttcg aagagagaca
     4021 ctacctgcaa ctattacctt cctctgcact tacacattcg ttaagtcata tatgtttttg
     4081 acttatccgc ttctactaga gattaaagag gagaaatact agatgagtac aggcatcgat
     4141 aaggacgtcc gagagtgttg gggcgtaact gcattatcag cgggtcatca aattgcaatg
     4201 aatagcgcgt ttctggatat ggacttgctg ttgtgcgggg aaaccggcac cggcaaggac
     4261 acactggcca accgcattca cgagttgtcc agcaggtcgg gaccctttgt gggcatgaac
     4321 tgcgccgcca ttcccgagtc gctggcagag agccagttat tcggtgtggt caacggtgca
     4381 ttcaccggcg tatgccgggc tcgcgagggc tacatagagg cctccagtgg tggcaccttg
     4441 tacctggatg aaatcgacag catgccgttg agcctgcaag ccaaactgct gcgtgtgttg
     4501 gagagtcgag gtatcgagcg tctgggctcg accgaattta tcccggtgga tctgcggatc
     4561 attgcctcgg cccagcggcc actggatgaa ctggtggaac aaggactttt ccgtcgcgac
     4621 ctgttttttc ggctcaacgt gctgacgctt cacttgccag ccttgcgcaa acgtcgtgaa
     4681 cagatcctgc cattgttcga ccagttcacc cagggtatcg ctgccgagtt cggacgtccc
     4741 gctcctgcgc tggacagcgg gcgtgtgcag ctgctgctca gccacgactg gccgggcaac
     4801 atccgcgaat tgaagtctgc ggccaagcgc ttcgtactcg gcttcccctt gctgggcgcc
     4861 gaccctgtgg aagcgcttga ccctgccacg gggctgcgca cgcaaatgcg catcatcgag
     4921 aaaatgctca tccaggatgc cttgaagcgg cacaggcaca atttcgacgc ggtgcttcag
     4981 gagttggagt tgccaagacg caccctgtat caccgcatga aggaactggg agttgcagcg
     5041 ccgatcgctg cgacggccgg ggtctaataa aacgcaggtg tggttatgct actagagatt
     5101 aaagaggaga aatactagat gatgagtctt gatgaaaggt ttgaggatga tctggacgag
     5161 gagcgggttc cgaatctggg gatagttgcc gaaagtattt cgcaactggg tatcgacgtg
     5221 ctgctatcgg gtgagaccgg cacgggcaaa gacacgattg cccgacggat tcatgagatg
     5281 tcaggccgca aagggcgcct ggtggcgatg aattgcgcgg ccattccgga gtccctcgcc
     5341 gagagcgagt tattcggcgt ggtcagcggt gcctacaccg gcgctgatcg ctccagagtc
     5401 ggttatgtcg aagcggcgca gggcggcacg ctgtacctgg atgagatcga tagcatgccg
     5461 ctgagcctgc aagccaaatt gctgagggtg ctggaaaccc gagcgcttga acggctgggt
     5521 tcgacgtcga cgatcaagct ggatatctgc gtgatcgcct ccgcccaatg ctcgctggac
     5581 gacgccgtcg agcgggggca gtttcgtcgc gatctgtatt ttcgcctgaa cgtcctgaca
     5641 ctcaagcttc ctccgctacg taaccagtct gatcgcatag ttcccctgtt cacacgtttt
     5701 acggccgccg ccgcgaggga gctcggtgtt cccgttcccg atgtttgccc actgctgcac
     5761 aaagtgctgc tgggccacga ctggcccggc aatatccgtg agctcaaggc ggcagccaaa
     5821 cgccatgtgc tgggtttccc cttgctgggc gccgagccgc agggcgaaga gcacttggcc
     5881 tgtgggctca aatcgcaatt gcgagtgatc gaaaaagccc tgattcagga gtcgctcaag
     5941 cgccacgaca attgtgtgga ttcggtaagc ctggaactgg acgtgccacg ccgtacgctc
     6001 tatcgacgca tcaaagaatt gcagatctaa taatactaga gccaggcatc aaataaaacg
     6061 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     6121 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc
     6181 cggattatgt ccgctgagtg ggtcacggtc ccggatcagt tcccttgcga agctgaccga
     6241 tgtttttgtg ccaaaagctg ttgtggcaaa aaacggtttg cgcaaagttt tgtattacaa
     6301 agaatttcac attttaaaat atctttataa atcaatcagt tatttctatt ttcaagctgg
     6361 cacggttatt gctatagggc ttgtactact agagattaaa gaggagaaat actagatgcg
     6421 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     6481 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     6541 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     6601 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     6661 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     6721 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     6781 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     6841 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     6901 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     6961 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     7021 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     7081 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     7141 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     7201 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     7261 tctgcgttta tatactagta gcggccgctg ca
//
