LOCUS       pSB123_-_pL0_rLUC-I_(CDS1)      3288 bp    DNA     circular UNA 3-MAR-2009
COMMENT     Confirmed by col. PCR and sequencing. 
            
FEATURES             Location/Qualifiers
     Vector          join(3273..3288,1..2231)
                     /origin="pICH41308 (2,845bp circular)Target vector: from
                     BpiI cut at 2829^2830 to BpiI cut at 2231^2232,
                     2,247bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     CDS             215..1225
                     /vntifkey="4"
                     /label="Sm/Sp no DraIII"
     mutation        1192
                     /vntifkey="62"
                     /label="G to A"
     misc_feature    1385..1999
                     /vntifkey="21"
                     /label="rep (pMB1)"
     rep_origin      complement(1984)
                     /vntifkey="33"
                     /label="ORI"
                     /note="RNaseH cleavage point"
     misc_feature    2225..2230
                     /label="AATG"
     misc_feature    2232..3272
                     /feature_type="Insertion"
                     /origin="rLuc codon op + intron_v3 (1,125bp
                     linear)Fragment: from BpiI cut at 40^41 to BpiI cut at
                     1081^1082, 1,041bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             join(2233..3072,3177..3272)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate
                     Renilla reniformis (sea pansy)"
                     /label="rLUC"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
                     /organism="synthetic DNA construct"
                     /label="rLUC-I"
     intron          3073..3176
                     /note="2nd intron from pICH75111"
     misc_feature    3273..3276
                     /vntifkey="21"
                     /label="GCTT"
ORIGIN
        1 GTGGCTCTTC AGTGGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
       61 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
      121 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
      181 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGCGC TCACGCAACT GGTCCAGAAC
      241 CTTGACCGAA CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG
      301 TTTTTTTGGG GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG
      361 TCGATGTTTG ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC
      421 AAAGTTAAAC ATCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT
      481 AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC
      541 CGCAGTGGAT GGCGGCCTGA AGCCACACAG CGATATTGAT TTGCTGGTTA CGGTGACCGT
      601 AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC
      661 CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT
      721 CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA
      781 CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC
      841 AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC
      901 GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC
      961 GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG
     1021 CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT
     1081 GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA
     1141 AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCATTACG TAAAAGGCGA
     1201 GATCACCAAG GTAGTCGGCA AATAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
     1261 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
     1321 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
     1381 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
     1441 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
     1501 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA
     1561 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
     1621 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
     1681 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
     1741 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
     1801 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
     1861 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
     1921 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
     1981 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
     2041 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
     2101 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
     2161 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATCACT
     2221 CTGTGGTCTC AAATGACTTC TAAGGTTTAT GATCCTGAAC AAAGAAAGAG AATGATTACT
     2281 GGACCTCAAT GGTGGGCTAG ATGTAAGCAA ATGAATGTTC TTGATTCTTT TATTAATTAT
     2341 TATGATTCTG AAAAGCATGC TGAAAATGCT GTTATTTTTC TTCATGGAAA TGCTGCTTCT
     2401 TCTTATCTTT GGAGACATGT TGTTCCACAT ATTGAACCAG TTGCTAGATG TATTATTCCA
     2461 GATCTTATTG GTATGGGAAA GTCTGGTAAG TCTGGTAATG GTTCTTATAG ACTTCTTGAT
     2521 CATTATAAGT ATCTTACTGC TTGGTTTGAA CTTCTTAATC TTCCAAAGAA GATTATTTTT
     2581 GTTGGACATG ATTGGGGTGC TTGTTTGGCT TTTCATTATT CTTATGAACA TCAAGATAAG
     2641 ATTAAGGCTA TTGTTCATGC TGAATCTGTT GTTGATGTTA TTGAATCTTG GGATGAATGG
     2701 CCTGATATTG AAGAAGATAT TGCTTTGATT AAGTCTGAAG AAGGAGAAAA GATGGTTTTG
     2761 GAAAATAATT TCTTTGTTGA AACTATGTTG CCATCTAAGA TTATGAGAAA GCTTGAACCA
     2821 GAAGAATTTG CTGCTTATCT TGAACCATTT AAGGAAAAGG GTGAAGTTAG AAGGCCAACT
     2881 CTTTCTTGGC CTAGAGAAAT TCCTCTTGTT AAGGGTGGTA AGCCTGATGT TGTTCAAATT
     2941 GTTAGAAATT ATAATGCTTA TCTTAGAGCT TCTGATGATC TTCCAAAGAT GTTTATTGAA
     3001 TCTGATCCAG GATTCTTTTC TAATGCTATT GTTGAAGGTG CTAAGAAGTT TCCTAATACT
     3061 GAATTTGTTA AGGTACTTCA TGCTTCAACG TGTAACTTAA GAGATACTGT GTGAAATTTT
     3121 ATATTTCCAT ACATTTGCTT GACCTTTGCT TTTTGTCAAT TTTTTTCCCC TTACAGGTTA
     3181 AGGGACTTCA TTTTTCTCAA GAAGATGCTC CTGATGAAAT GGGAAAGTAT ATTAAGTCTT
     3241 TTGTTGAAAG AGTTCTTAAG AATGAACAAT GAGCTTTGAG ACCACGAA
//
