LOCUS       pSB115_-_pL2_pSB90_fLUC-I_tGFP-I     10903 bp    DNA     circular SYN 21-NOV-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAGM4723 cor
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2013 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Confirmed by HindIII and NdeI digest. 
FEATURES             Location/Qualifiers
     Vector          join(5074..10903,1)
                     /origin="pSB90 - pAGM4723_VirGN54D (NEBuilder) (14,072bp
                     circular)Target vector: from BbsI cut at 8096^8097 to
                     BbsI cut at 1^2, 5,977bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     misc_feature    2..20
                     /feature_type="Insertion"
                     /origin="pICH54011 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    21..2057
                     /feature_type="Insertion"
                     /origin="pSB112 - pL1F2_2x35S::tGFP-I::Tnos (6,389bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 6372^6373, 2,037bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(2058..>2061,<25..40))
                     /origin="pICH47742 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     misc_feature    37..40
                     /label="GGAG 4bp overhang"
     promoter        41..793
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(41..883)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           810..871
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    880..1786
                     /feature_type="Insertion"
                     /origin="tGFP-I (CDS1)  (931bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 917^918, 907bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             join(881..1261,1451..1786)
                     /label="turbo GFP (Pontellina plumata)"
     misc_feature    complement(884..1790)
                     /feature_type="Insertion"
                     /origin="pSB65 - pL0_tGFP-I (CDS1) (3,154bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 3138^3139, 907bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          1262..1450
                     /label="intron (U5 small nuclear ribonucleoprotein
                     component (Arabidopsis thaliana))"
     CDS             1787..1790
                     /vntifkey="4"
                     /label="GCTT"
     misc_feature    complement(1791..2057)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      1791..2053
                     /vntifkey="43"
                     /label="nost"
     misc_feature    2058..2076
                     /feature_type="Insertion"
                     /origin="pICH54033 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    2077..5049
                     /feature_type="Insertion"
                     /origin="pSB72 - pL1F4_2x35S::fLUC-I::Tnos (7,325bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 7308^7309, 2,973bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(5050..>5053,<2081..2096))
                     /origin="pICH47761 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        2097..2849
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(2097..2939)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           2866..2927
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     MIsc._Feature   <2936..2939
                     /label="AATG 4bp overhang"
     misc_feature    2936..3080
                     /feature_type="Insertion"
                     /origin="fLuc part1 (169bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 155^156, 145bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             2937..3080
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(2940..>3084)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(2940..4782)
                     /feature_type="Insertion"
                     /origin="pSB63 - pL0_fLuc-I (CDS1) (4,090bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 4074^4075, 1,843bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          3081..3269
                     /note="U5 small nuclear ribonucleoprotein component
                     (Arabidopsis thaliana)"
     misc_feature    3081..3265
                     /feature_type="Insertion"
                     /origin="fLuc part2 (209bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 195^196, 185bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(<3085..>3269)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    3266..4778
                     /feature_type="Insertion"
                     /origin="fLuc part3 (1,537bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 1523^1524,
                     1,513bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             3270..4778
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(<3270..>4782)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(4783..5049)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      4783..5045
                     /vntifkey="43"
                     /label="nost"
     misc_feature    5050..5073
                     /feature_type="Insertion"
                     /origin="pICH41780 (3,318bp circular)Fragment: from BbsI
                     cut at 2847^2848 to BbsI cut at 2871^2872,
                     24bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    5054..5073
                     /vntifkey="21"
                     /label="EL4"
     misc_feature    complement(5091..5218)
                     /label="RB"
                     /note="RB"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    5223..7857
                     /label="pVS1 fragment short"
                     /note="pVS1 fragment short"
                     /note="color: #66cc99; direction: RIGHT"
     CDS             5413..6042
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 StaA"
     CDS             6476..7543
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 RepA"
     rep_origin      7609..7803
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     promoter        7865..7969
                     /label="AmpR promoter"
     CDS             7970..8761
                     /codon_start=1
                     /label="NPTII"
                     /note="NPTII"
                     /note="color: #ff9966"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       8452
                     /note="A to G (BpiI removed)"
                     /note="color: #3366cc"
     variation       8651
                     /note="A to C, I to L"
                     /note="color: #3366cc"
     misc_feature    8894..10046
                     /label="VirGN54D insert"
     CDS             9024..9827
                     /label="VirGN54D"
     misc_feature    10077..10691
                     /label="rep - pMB1"
                     /note="rep - pMB1"
                     /note="color: #66cc99; direction: RIGHT"
     variation       10235
                     /note="C to T"
                     /note="color: #3366cc"
     rep_origin      10676
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     misc_feature    complement(10743..10893)
                     /label="LB"
                     /note="LB"
                     /note="color: #66cc99; direction: LEFT"
ORIGIN
        1 GTGCCGATGT ATCACCTCTC GCAAGAATTC AAGCTTGGAG GTCAACATGG TGGAGCACGA
       61 CACTCTGGTC TACTCCAAAA ATGTCAAAGA TACAGTCTCA GAAGATCAAA GGGCTATTGA
      121 GACTTTTCAA CAAAGGATAA TTTCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG
      181 TCACTTCATC GAAAGGACAG TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA
      241 TAAAGGAAAG GCTATCATTC AAGATCTCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC
      301 ACCCACGAGG AGCATCGTGG AAAAAGAAGA GGTTCCAACC ACGTCTACAA AGCAAGTGGA
      361 TTGATGTGAT AACATGGTGG AGCACGACAC TCTGGTCTAC TCCAAAAATG TCAAAGATAC
      421 AGTCTCAGAA GATCAAAGGG CTATTGAGAC TTTTCAACAA AGGATAATTT CGGGAAACCT
      481 CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTCATCGAA AGGACAGTAG AAAAGGAAGG
      541 TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCATTCAAG ATCTCTCTGC
      601 CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGAGGT
      661 TCCAACCACG TCTACAAAGC AAGTGGATTG ATGTGACATC TCCACTGACG TAAGGGATGA
      721 CGCACAATCC CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT
      781 GGAGAGGACA CGCTCGAGTA TAAGAGCTCA TTTTTACAAC AATTACCAAC AACAACAAAC
      841 AACAAACAAC ATTACAATTA CATTTACAAT TATCGATACA ATGAGAGGAT CTGGATCTGA
      901 GTCTGATGAG TCTGGACTTC CTGCTATGGA AATCGAGTGT AGAATCACTG GAACCCTTAA
      961 CGGTGTTGAG TTCGAGCTTG TTGGAGGTGG TGAGGGAACT CCTGAGCAGG GAAGAATGAC
     1021 TAACAAGATG AAGTCTACCA AGGGTGCTCT TACCTTCTCT CCATACCTTC TTTCTCACGT
     1081 TATGGGATAC GGATTCTACC ACTTCGGAAC TTACCCATCT GGATACGAGA ACCCTTTCCT
     1141 TCATGCTATC AACAACGGTG GATACACCAA CACTAGGATC GAGAAGTACG AGGATGGTGG
     1201 TGTTCTTCAC GTTAGCTTCT CTTACAGATA CGAGGCTGGA AGAGTGATCG GAGATTTCAA
     1261 GGTAAGTTTC TGCTTCTACC TTTGATATAT ATATAATAAT TATCATTAAT TAGTAGTAAT
     1321 ATAATATTTC AAATATTTTT TTCAAAATAA AAGAATGTAG TATATAGCAA TTGCTTTTCT
     1381 GTAGTTTATA AGTGTGTATA TTTTAATTTA TAACTTTTCT AATATATGAC CAAAATTTGT
     1441 TGATGTGCAG GTTATGGGAA CTGGATTCCC TGAGGATTCT GTTATCTTCA CCGACAAGAT
     1501 CATCAGGTCT AACGCTACTG TTGAGCATCT TCATCCTATG GGAGATAACG ATCTCGATGG
     1561 ATCTTTCACC AGAACCTTCT CACTTAGAGA TGGTGGTTAC TACTCTTCTG TGGTGGATTC
     1621 TCACATGCAC TTCAAGTCTG CTATCCACCC TTCTATCCTT CAAAACGGTG GACCTATGTT
     1681 CGCTTTCAGA AGAGTTGAGG AAGATCACTC TAACACCGAG CTTGGAATCG TTGAGTACCA
     1741 ACATGCTTTC AAGACCCCTG ATGCTGATGC TGGTGAGGAA TGATAAGCTT GTCAAGCAGA
     1801 TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT
     1861 GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT
     1921 GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC
     1981 GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT
     2041 GTTACTAGAT CGACGCTACT AGCAACATAC GCTGGATTAC GAATTCCCAT GGGGAGGTCA
     2101 ACATGGTGGA GCACGACACT CTGGTCTACT CCAAAAATGT CAAAGATACA GTCTCAGAAG
     2161 ATCAAAGGGC TATTGAGACT TTTCAACAAA GGATAATTTC GGGAAACCTC CTCGGATTCC
     2221 ATTGCCCAGC TATCTGTCAC TTCATCGAAA GGACAGTAGA AAAGGAAGGT GGCTCCTACA
     2281 AATGCCATCA TTGCGATAAA GGAAAGGCTA TCATTCAAGA TCTCTCTGCC GACAGTGGTC
     2341 CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAGAGGTT CCAACCACGT
     2401 CTACAAAGCA AGTGGATTGA TGTGATAACA TGGTGGAGCA CGACACTCTG GTCTACTCCA
     2461 AAAATGTCAA AGATACAGTC TCAGAAGATC AAAGGGCTAT TGAGACTTTT CAACAAAGGA
     2521 TAATTTCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCGAAAGGA
     2581 CAGTAGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCA
     2641 TTCAAGATCT CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG
     2701 TGGAAAAAGA AGAGGTTCCA ACCACGTCTA CAAAGCAAGT GGATTGATGT GACATCTCCA
     2761 CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG
     2821 GAAGTTCATT TCATTTGGAG AGGACACGCT CGAGTATAAG AGCTCATTTT TACAACAATT
     2881 ACCAACAACA ACAAACAACA AACAACATTA CAATTACATT TACAATTATC GATACAATGG
     2941 AGGACGCCAA AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCTCTAGAG GATGGAACCG
     3001 CTGGAGAGCA ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT
     3061 TTACAGATGC ACATATCGAG GTAAGTTTCT GCTTCTACCT TTGATATATA TATAATAATT
     3121 ATCATTAATT AGTAGTAATA TAATATTTCA AATATTTTTT TCAAAATAAA AGAATGTAGT
     3181 ATATAGCAAT TGCTTTTCTG TAGTTTATAA GTGTGTATAT TTTAATTTAT AACTTTTCTA
     3241 ATATATGACC AAAATTTGTT GATGTGCAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
     3301 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT CACAGAATCG
     3361 TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT GTTGGGCGCG TTATTTATCG
     3421 GAGTTGCAGT TGCGCCCGCG AACGACATTT ATAATGAACG TGAATTGCTC AACAGTATGA
     3481 ACATTTCGCA GCCTACCGTA GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG
     3541 TGCAAAAAAA ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
     3601 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT TTTAATGAAT
     3661 ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT TGCACTGATA ATGAATTCCT
     3721 CTGGATCTAC TGGGTTACCT AAGGGTGTGG CCCTTCCGCA TAGAACTGCC TGCGTCAGAT
     3781 TCTCGCATGC CAGAGATCCT ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA
     3841 GTGTTGTTCC ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
     3901 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA TCCCTTCAGG
     3961 ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT TTCATTCTTC GCCAAAAGCA
     4021 CTCTGATTGA CAAATACGAT TTATCTAATT TACACGAAAT TGCTTCTGGG GGCGCACCTC
     4081 TTTCGAAAGA AGTCGGGGAA GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG
     4141 GATATGGGCT CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
     4201 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT CTGGATACCG
     4261 GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT CAGAGGACCT ATGATTATGT
     4321 CCGGTTATGT AAACAATCCG GAAGCGACCA ACGCCTTGAT TGACAAGGAT GGATGGCTAC
     4381 ATTCTGGAGA CATAGCTTAC TGGGACGAGG ACGAACACTT CTTCATAGTT GACCGCTTGA
     4441 AGTCTTTAAT TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
     4501 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT GCCCGACGAT GACGCCGGTG
     4561 AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC GATGACGGAA AAAGAGATCG
     4621 TGGATTACGT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG
     4681 TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC
     4741 TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGTAAGC TTGTCAAGCA GATCGTTCAA
     4801 ACATTTGGCA ATAAAGTTTC TTAAGATTGA ATCCTGTTGC CGGTCTTGCG ATGATTATCA
     4861 TATAATTTCT GTTGAATTAC GTTAAGCATG TAATAATTAA CATGTAATGC ATGACGTTAT
     4921 TTATGAGATG GGTTTTTATG ATTAGAGTCC CGCAATTATA CATTTAATAC GCGATAGAAA
     4981 ACAAAATATA GCGCGCAAAC TAGGATAAAT TATCGCGCGC GGTGTCATCT ATGTTACTAG
     5041 ATCGACGCTC AGAGAGGATG CACATGTGAC CGAGGGACAC GAAGTGATCC GTTTAAACTA
     5101 TCAGTGTTTG ACAGGATATA TTGGCGGGTA AACCTAAGAG AAAAGAGCGT TTATTAGAAT
     5161 AATCGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCCAGC
     5221 CGTGCGGCTG CATGAAATCC TGGCCGGTTT GTCTGATGCC AAGCTGGCGG CCTGGCCGGC
     5281 CAGCTTGGCC GCTGAAGAAA CCGAGCGCCG CCGTCTAAAA AGGTGATGTG TATTTGAGTA
     5341 AAACAGCTTG CGTCATGCGG TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG
     5401 CAAGGGGAAC GCATGAAGGT TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG
     5461 ACCATCGCAA CCCATCTAGC CCGCGCCCTG CAACTCGCCG GGGCCGATGT TCTGTTAGTC
     5521 GATTCCGATC CCCAGGGCAG TGCCCGCGAT TGGGCGGCCG TGCGGGAAGA TCAACCGCTA
     5581 ACCGTTGTCG GCATCGACCG CCCGACGATT GACCGCGACG TGAAGGCCAT CGGCCGGCGC
     5641 GACTTCGTAG TGATCGACGG AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG
     5701 GCAGCCGACT TCGTGCTGAT TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC
     5761 GACCTGGTGG AGCTGGTTAA GCAGCGCATT GAGGTCACGG ATGGAAGGCT ACAAGCGGCC
     5821 TTTGTCGTGT CGCGGGCGAT CAAAGGCACG CGCATCGGCG GTGAGGTTGC CGAGGCGCTG
     5881 GCCGGGTACG AGCTGCCCAT TCTTGAGTCC CGTATCACGC AGCGCGTGAG CTACCCAGGC
     5941 ACTGCCGCCG CCGGCACAAC CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG
     6001 GTCCAGGCGC TGGCCGCTGA AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA
     6061 AAATGAGCAA AAGCACAAAC ACGCTAAGTG CCGGCCGTCC GAGCGCACGC AGCAGCAAGG
     6121 CTGCAACGTT GGCCAGCCTG GCAGACACGC CAGCCATGAA GCGGGTCAAC TTTCAGTTGC
     6181 CGGCGGAGGA TCACACCAAG CTGAAGATGT ACGCGGTACG CCAAGGCAAG ACCATTACCG
     6241 AGCTGCTATC TGAATACATC GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT
     6301 AGATGAATTT TAGCGGCTAA AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC
     6361 GCCGTGGAAT GCCCCATGTG TGGAGGAACG GGCGGTTGGC CAGGCGTAAG CGGCTGGGTT
     6421 GTCTGCCGGC CCTGCAATGG CACTGGAACC CCCAAGCCCG AGGAATCGGC GTGACGGTCG
     6481 CAAACCATCC GGCCCGGTAC AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT
     6541 GAAGGCCGCG CAGGCCGCCC AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC
     6601 GTGGCAAGCG GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC
     6661 GCCGTCGATT AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT
     6721 CTATGACGTG GGCACCCGCG ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC
     6781 GAAGCGTGAC CGACGAGCTG GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA
     6841 GGTTTCCGCA GGGCCGGCCG GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC
     6901 GGTTTCCCAT CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG
     6961 CCGCGTGTTC CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG
     7021 AAAGCAGAAA GACGACCTGG TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT
     7081 GCAGCGTACG AAGAAGGCCA AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT
     7141 GATTAGCCGC TACAAGATCG TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA
     7201 GCTAGCTGAT TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT
     7261 TCACCCCGAT TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG
     7321 CCGCGCCGCA GGCAAGGCAG AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG
     7381 CAGCGCCGGA GAGTTCAAGA AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA
     7441 CCTGCCGGAG TACGATTTGA AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG
     7501 CTACCGCAAC CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT
     7561 AGGGCAAATT GCCCTAGCAG GGGAAAAAGG TCGAAAAAGC TTCTTTCCTG TGGATAGCAC
     7621 GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA
     7681 GCCGTACATT GGGAACCGGT CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA
     7741 TTTTTCCGCC TAAAACTCTT TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC
     7801 ATAACTGTCT GGCCAGCGCA CAGCCGAACA GCTGCAAAAA GCGCCCACAT CAAGGCTCCG
     7861 AGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     7921 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGGCTAAAAT
     7981 GAGAATATCA CCGGAATTGA AAAAACTGAT CGAAAAATAC CGCTGCGTAA AAGATACGGA
     8041 AGGAATGTCT CCTGCTAAGG TATATAAGCT GGTGGGAGAA AATGAAAACC TATATTTAAA
     8101 AATGACGGAC AGCCGGTATA AAGGGACCAC CTATGATGTG GAACGGGAAA AGGACATGAT
     8161 GCTATGGCTG GAAGGAAAGC TGCCTGTTCC AAAGGTCCTG CACTTTGAAC GGCATGATGG
     8221 CTGGAGCAAT CTGCTCATGA GTGAGGCCGA TGGCGTCCTT TGCTCGGAAG AGTATGAAGA
     8281 TGAACAAAGC CCTGAAAAGA TTATCGAGCT GTATGCGGAG TGCATCAGGC TCTTTCACTC
     8341 CATCGACATA TCGGATTGTC CCTATACGAA TAGCTTAGAC AGCCGCTTAG CCGAATTGGA
     8401 TTACTTACTG AATAACGATC TGGCCGATGT GGATTGCGAA AACTGGGAAG AGGACACTCC
     8461 ATTTAAAGAT CCGCGCGAGC TGTATGATTT TTTAAAGACG GAAAAGCCCG AAGAGGAACT
     8521 TGTCTTTTCC CACGGCGACC TGGGAGACAG CAACATCTTT GTGAAAGATG GCAAAGTAAG
     8581 TGGCTTTATT GATCTTGGGA GAAGCGGCAG GGCGGACAAG TGGTATGACA TTGCCTTCTG
     8641 CGTCCGGTCG CTCAGGGAGG ATATCGGGGA AGAACAGTAT GTCGAGCTAT TTTTTGACTT
     8701 ACTGGGGATC AAGCCTGATT GGGAGAAAAT AAAATATTAT ATTTTACTGG ATGAATTGTT
     8761 TTAGCTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
     8821 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
     8881 TGAGTTTTCG TTCTAGCTGT AACCTCGAAG CGTTTCACTT GTAACAACGA TTGAGAATTT
     8941 TGTCATAAAA TTGAAATACT TGGTTCGCAT TTTCGTCATC CGCGGTCAGC CGCAATTCTG
     9001 ACGAACTGCC CATTTAGCTG GAGATGATTG TACATCCTTC ACGTGAAAAT TTCTCAAGCG
     9061 CTGTGAACAA GGGTTCAGAT TTTAGATTGA AAGGTGAGCC GTTGAAACAC GTTCTTCTTG
     9121 TCGATGACGA CGTCGCTATG CGGCATCTTA TTATTGAATA CCTTACGATC CACGCCTTCA
     9181 AAGTGACCGC GGTAGCCGAC AGCACCCAGT TCACAAGAGT ACTCTCTTCC GCGACGGTCG
     9241 ATGTCGTGGT TGTTGATCTA GATTTAGGTC GTGAAGATGG GCTCGAGATC GTTCGTAATC
     9301 TGGCGGCAAA GTCTGATATT CCAATCATAA TTATCAGTGG CGACCGCCTT GAGGAGACGG
     9361 ATAAAGTTGT TGCACTCGAG CTAGGAGCAA GTGATTTTAT CGCTAAGCCG TTCAGTATCA
     9421 GAGAGTTTCT AGCACGCATT CGGGTTGCCT TGCGCGTGCG CCCCAACGTT GTCCGCTCCA
     9481 AAGACCGACG GTCTTTTTGT TTTACTGACT GGACACTTAA TCTCAGGCAA CGTCGCTTGA
     9541 TGTCCGAAGC TGGCGGTGAG GTGAAACTTA CGGCAGGTGA GTTCAATCTT CTCCTCGCGT
     9601 TTTTAGAGAA ACCCCGCGAC GTTCTATCGC GCGAGCAACT TCTCATTGCC AGTCGAGTAC
     9661 GCGACGAGGA GGTTTATGAC AGGAGTATAG ATGTTCTCAT TTTGAGGCTG CGCCGCAAAC
     9721 TTGAGGCGGA TCCGTCAAGC CCTCAACTGA TAAAAACAGC AAGAGGTGCC GGTTATTTCT
     9781 TTGACGCGGA CGTGCAGGTT TCGCACGGGG GGACGATGGC AGCCTGAGCC AATTGCATTT
     9841 GCCTCTTAAT TATCTGGCTC AAAGGGTGAC TGAGGAGTAA GCGATGTGCC CATCACACTG
     9901 ACCACCAAGA CGGGGGTTCG TTTTGAAGAT CACCCTTCAG ACCCAAGGCC AGTGTGCGTT
     9961 GATCGCGGCC ACACTGCAAA TTGACATACT GTCGATAAAG GGGCGTTGGA CGAGAACCCA
    10021 TTCGTCAGGA TACTGGCTCG TGATCTCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
    10081 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
    10141 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
    10201 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC
    10261 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
    10321 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
    10381 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
    10441 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
    10501 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
    10561 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
    10621 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
    10681 CCAGCAACGC GGCCTTTTTA CGGTTCCTGC TCGGATCTGT TGGACCGGAC AGTAGTCATG
    10741 GTTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT
    10801 TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT TGACGCTTAG ACAACTTAAT
    10861 AACACATTGC GGACGTTTTT AATGTACTGG GGTTGAACAC TCT
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