LOCUS       pSB116_-_pL2_pSB90_fLUC-I_P19     10516 bp    DNA     circular SYN 21-NOV-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAGM4723 cor
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2013 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Confirmed by HindIII and NdeI digest. 
FEATURES             Location/Qualifiers
     Vector          join(4687..10516,1)
                     /origin="pSB90 - pAGM4723_VirGN54D (NEBuilder) (14,072bp
                     circular)Target vector: from BbsI cut at 8096^8097 to
                     BbsI cut at 1^2, 5,977bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     misc_feature    2..1651
                     /feature_type="Insertion"
                     /origin="pSB68 - pL1F1_2x35S::P19::Tnos (6,002bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 5985^5986, 1,650bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(1652..>1655,<6..21))
                     /origin="pICH47732 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        22..774
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(22..864)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           791..852
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     CDS             862..1380
                     /vntifkey="4"
                     /label="P19 (no Esp3I)"
                     /label="P19 (TBSV)"
     misc_feature    complement(865..1384)
                     /feature_type="Insertion"
                     /origin="pICH44022 (2,767bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     520bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(1385..1651)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      1385..1647
                     /vntifkey="43"
                     /label="nost"
     misc_feature    1652..1670
                     /feature_type="Insertion"
                     /origin="pICH54022 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    1671..1689
                     /feature_type="Insertion"
                     /origin="pICH54033 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    1690..4662
                     /feature_type="Insertion"
                     /origin="pSB72 - pL1F4_2x35S::fLUC-I::Tnos (7,325bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 7308^7309, 2,973bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(4663..>4666,<1694..1709))
                     /origin="pICH47761 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        1710..2462
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(1710..2552)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           2479..2540
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    2549..2693
                     /feature_type="Insertion"
                     /origin="fLuc part1 (169bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 155^156, 145bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             2550..2693
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(2553..>2697)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(2553..4395)
                     /feature_type="Insertion"
                     /origin="pSB63 - pL0_fLuc-I (CDS1) (4,090bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 4074^4075, 1,843bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          2694..2882
                     /note="U5 small nuclear ribonucleoprotein component
                     (Arabidopsis thaliana)"
     misc_feature    2694..2878
                     /feature_type="Insertion"
                     /origin="fLuc part2 (209bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 195^196, 185bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(<2698..>2882)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    2879..4391
                     /feature_type="Insertion"
                     /origin="fLuc part3 (1,537bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 1523^1524,
                     1,513bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             2883..4391
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(<2883..>4395)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(4396..4662)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      4396..4658
                     /vntifkey="43"
                     /label="nost"
     misc_feature    4663..4686
                     /feature_type="Insertion"
                     /origin="pICH41780 (3,318bp circular)Fragment: from BbsI
                     cut at 2847^2848 to BbsI cut at 2871^2872,
                     24bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    4667..4686
                     /vntifkey="21"
                     /label="EL4"
     misc_feature    complement(4704..4831)
                     /label="RB"
                     /note="RB"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    4836..7470
                     /label="pVS1 fragment short"
                     /note="pVS1 fragment short"
                     /note="color: #66cc99; direction: RIGHT"
     CDS             5026..5655
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 StaA"
     CDS             6089..7156
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 RepA"
     rep_origin      7222..7416
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     promoter        7478..7582
                     /label="AmpR promoter"
     CDS             7583..8374
                     /codon_start=1
                     /label="NPTII"
                     /note="NPTII"
                     /note="color: #ff9966"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       8065
                     /note="A to G (BpiI removed)"
                     /note="color: #3366cc"
     variation       8264
                     /note="A to C, I to L"
                     /note="color: #3366cc"
     misc_feature    8507..9659
                     /label="VirGN54D insert"
     CDS             8637..9440
                     /label="VirGN54D"
     misc_feature    9690..10304
                     /label="rep - pMB1"
                     /note="rep - pMB1"
                     /note="color: #66cc99; direction: RIGHT"
     variation       9848
                     /note="C to T"
                     /note="color: #3366cc"
     rep_origin      10289
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     misc_feature    complement(10356..10506)
                     /label="LB"
                     /note="LB"
                     /note="color: #66cc99; direction: LEFT"
ORIGIN
        1 GTGCCGAATT CGGATCCGGA GGTCAACATG GTGGAGCACG ACACTCTGGT CTACTCCAAA
       61 AATGTCAAAG ATACAGTCTC AGAAGATCAA AGGGCTATTG AGACTTTTCA ACAAAGGATA
      121 ATTTCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CGAAAGGACA
      181 GTAGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCATT
      241 CAAGATCTCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG
      301 GAAAAAGAAG AGGTTCCAAC CACGTCTACA AAGCAAGTGG ATTGATGTGA TAACATGGTG
      361 GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA CAGTCTCAGA AGATCAAAGG
      421 GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC TCCTCGGATT CCATTGCCCA
      481 GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG GTGGCTCCTA CAAATGCCAT
      541 CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG CCGACAGTGG TCCCAAAGAT
      601 GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGAGG TTCCAACCAC GTCTACAAAG
      661 CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT
      721 TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTCGAGT
      781 ATAAGAGCTC ATTTTTACAA CAATTACCAA CAACAACAAA CAACAAACAA CATTACAATT
      841 ACATTTACAA TTATCGATAC AATGGAACGA GCTATACAAG GAAACGATGC TAGGGAACAA
      901 GCTTATGGTG AACGTTGGAA TGGAGGATCA GGAAGTTCCA CTTCTCCCTT CAAACTTCCT
      961 GACGAAAGTC CGAGTTGGAC TGAGTGGCGG CTACATAACG ATGAGACTAT TTCGAATCAA
     1021 GATAATCCCC TTGGTTTCAA GGAAAGCTGG GGTTTCGGGA AAGTTGTATT TAAGAGATAT
     1081 CTCAGATACG ACGGGACGGA AACTTCACTG CACAGAGTCC TTGGATCTTG GACGGGAGAT
     1141 TCGGTTAACT ATGCAGCATC TCGATTTCTC GGTTTCGACC AGATCGGATG TACCTATAGT
     1201 ATTCGGTTTC GAGGAGTTAG TGTCACCATT TCTGGAGGGT CGCGAACTCT TCAGCATCTC
     1261 AGTGAAATGG CAATTCGGTC TAAGCAAGAA CTGCTACAGC TTACCCCAGT CAAAGTGGAA
     1321 AGTGATGTAT CAAGAGGATG CCCTGAAGGT GTTGAAACCT TCGAAGAAGA AAGCGAGTAA
     1381 GCTTGTCAAG CAGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT
     1441 GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT
     1501 AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA
     1561 TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC
     1621 GCGGTGTCAT CTATGTTACT AGATCGACGC TGCAAGGTTT TGACGGGTTT ACTAGCAACA
     1681 TACGCTGGAT TACGAATTCC CATGGGGAGG TCAACATGGT GGAGCACGAC ACTCTGGTCT
     1741 ACTCCAAAAA TGTCAAAGAT ACAGTCTCAG AAGATCAAAG GGCTATTGAG ACTTTTCAAC
     1801 AAAGGATAAT TTCGGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTCATCG
     1861 AAAGGACAGT AGAAAAGGAA GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG
     1921 CTATCATTCA AGATCTCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA
     1981 GCATCGTGGA AAAAGAAGAG GTTCCAACCA CGTCTACAAA GCAAGTGGAT TGATGTGATA
     2041 ACATGGTGGA GCACGACACT CTGGTCTACT CCAAAAATGT CAAAGATACA GTCTCAGAAG
     2101 ATCAAAGGGC TATTGAGACT TTTCAACAAA GGATAATTTC GGGAAACCTC CTCGGATTCC
     2161 ATTGCCCAGC TATCTGTCAC TTCATCGAAA GGACAGTAGA AAAGGAAGGT GGCTCCTACA
     2221 AATGCCATCA TTGCGATAAA GGAAAGGCTA TCATTCAAGA TCTCTCTGCC GACAGTGGTC
     2281 CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAGAGGTT CCAACCACGT
     2341 CTACAAAGCA AGTGGATTGA TGTGACATCT CCACTGACGT AAGGGATGAC GCACAATCCC
     2401 ACTATCCTTC GCAAGACCCT TCCTCTATAT AAGGAAGTTC ATTTCATTTG GAGAGGACAC
     2461 GCTCGAGTAT AAGAGCTCAT TTTTACAACA ATTACCAACA ACAACAAACA ACAAACAACA
     2521 TTACAATTAC ATTTACAATT ATCGATACAA TGGAGGACGC CAAAAACATA AAGAAAGGCC
     2581 CGGCGCCATT CTATCCTCTA GAGGATGGAA CCGCTGGAGA GCAACTGCAT AAGGCTATGA
     2641 AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA TGCACATATC GAGGTAAGTT
     2701 TCTGCTTCTA CCTTTGATAT ATATATAATA ATTATCATTA ATTAGTAGTA ATATAATATT
     2761 TCAAATATTT TTTTCAAAAT AAAAGAATGT AGTATATAGC AATTGCTTTT CTGTAGTTTA
     2821 TAAGTGTGTA TATTTTAATT TATAACTTTT CTAATATATG ACCAAAATTT GTTGATGTGC
     2881 AGGTGAACAT CACGTACGCG GAATACTTCG AAATGTCCGT TCGGTTGGCA GAAGCTATGA
     2941 AACGATATGG GCTGAATACA AATCACAGAA TCGTCGTATG CAGTGAAAAC TCTCTTCAAT
     3001 TCTTTATGCC GGTGTTGGGC GCGTTATTTA TCGGAGTTGC AGTTGCGCCC GCGAACGACA
     3061 TTTATAATGA ACGTGAATTG CTCAACAGTA TGAACATTTC GCAGCCTACC GTAGTGTTTG
     3121 TTTCCAAAAA GGGGTTGCAA AAAATTTTGA ACGTGCAAAA AAAATTACCA ATAATCCAGA
     3181 AAATTATTAT CATGGATTCT AAAACGGATT ACCAGGGATT TCAGTCGATG TACACGTTCG
     3241 TCACATCTCA TCTACCTCCC GGTTTTAATG AATACGATTT TGTACCAGAG TCCTTTGATC
     3301 GTGACAAAAC AATTGCACTG ATAATGAATT CCTCTGGATC TACTGGGTTA CCTAAGGGTG
     3361 TGGCCCTTCC GCATAGAACT GCCTGCGTCA GATTCTCGCA TGCCAGAGAT CCTATTTTTG
     3421 GCAATCAAAT CATTCCGGAT ACTGCGATTT TAAGTGTTGT TCCATTCCAT CACGGTTTTG
     3481 GAATGTTTAC TACACTCGGA TATTTGATAT GTGGATTTCG AGTCGTCTTA ATGTATAGAT
     3541 TTGAAGAAGA GCTGTTTTTA CGATCCCTTC AGGATTACAA AATTCAAAGT GCGTTGCTAG
     3601 TACCAACCCT ATTTTCATTC TTCGCCAAAA GCACTCTGAT TGACAAATAC GATTTATCTA
     3661 ATTTACACGA AATTGCTTCT GGGGGCGCAC CTCTTTCGAA AGAAGTCGGG GAAGCGGTTG
     3721 CAAAACGCTT CCATCTTCCA GGGATACGAC AAGGATATGG GCTCACTGAG ACTACATCAG
     3781 CTATTCTGAT TACACCCGAG GGGGATGATA AACCGGGCGC GGTCGGTAAA GTTGTTCCAT
     3841 TTTTTGAAGC GAAGGTTGTG GATCTGGATA CCGGGAAAAC GCTGGGCGTT AATCAGAGAG
     3901 GCGAATTATG TGTCAGAGGA CCTATGATTA TGTCCGGTTA TGTAAACAAT CCGGAAGCGA
     3961 CCAACGCCTT GATTGACAAG GATGGATGGC TACATTCTGG AGACATAGCT TACTGGGACG
     4021 AGGACGAACA CTTCTTCATA GTTGACCGCT TGAAGTCTTT AATTAAATAC AAAGGATATC
     4081 AGGTGGCCCC CGCTGAATTG GAATCGATAT TGTTACAACA CCCCAACATC TTCGACGCGG
     4141 GCGTGGCAGG TCTGCCCGAC GATGACGCCG GTGAACTTCC CGCCGCCGTT GTTGTTTTGG
     4201 AGCACGGAAA GACGATGACG GAAAAAGAGA TCGTGGATTA CGTCGCCAGT CAAGTAACAA
     4261 CCGCGAAAAA GTTGCGCGGA GGAGTTGTGT TTGTGGACGA AGTACCGAAA GGTCTTACCG
     4321 GAAAACTCGA CGCAAGAAAA ATCAGAGAGA TCCTCATAAA GGCCAAGAAG GGCGGAAAGT
     4381 CCAAATTGTA AGCTTGTCAA GCAGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT
     4441 TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC
     4501 ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG
     4561 TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA
     4621 AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGACG CTCAGAGAGG ATGCACATGT
     4681 GACCGAGGGA CACGAAGTGA TCCGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
     4741 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
     4801 GGTTTATCCG TTCGTCCATT TGTATGTGCC AGCCGTGCGG CTGCATGAAA TCCTGGCCGG
     4861 TTTGTCTGAT GCCAAGCTGG CGGCCTGGCC GGCCAGCTTG GCCGCTGAAG AAACCGAGCG
     4921 CCGCCGTCTA AAAAGGTGAT GTGTATTTGA GTAAAACAGC TTGCGTCATG CGGTCGCTGC
     4981 GTATATGATG CGATGAGTAA ATAAACAAAT ACGCAAGGGG AACGCATGAA GGTTATCGCT
     5041 GTACTTAACC AGAAAGGCGG GTCAGGCAAG ACGACCATCG CAACCCATCT AGCCCGCGCC
     5101 CTGCAACTCG CCGGGGCCGA TGTTCTGTTA GTCGATTCCG ATCCCCAGGG CAGTGCCCGC
     5161 GATTGGGCGG CCGTGCGGGA AGATCAACCG CTAACCGTTG TCGGCATCGA CCGCCCGACG
     5221 ATTGACCGCG ACGTGAAGGC CATCGGCCGG CGCGACTTCG TAGTGATCGA CGGAGCGCCC
     5281 CAGGCGGCGG ACTTGGCTGT GTCCGCGATC AAGGCAGCCG ACTTCGTGCT GATTCCGGTG
     5341 CAGCCAAGCC CTTACGACAT ATGGGCCACC GCCGACCTGG TGGAGCTGGT TAAGCAGCGC
     5401 ATTGAGGTCA CGGATGGAAG GCTACAAGCG GCCTTTGTCG TGTCGCGGGC GATCAAAGGC
     5461 ACGCGCATCG GCGGTGAGGT TGCCGAGGCG CTGGCCGGGT ACGAGCTGCC CATTCTTGAG
     5521 TCCCGTATCA CGCAGCGCGT GAGCTACCCA GGCACTGCCG CCGCCGGCAC AACCGTTCTT
     5581 GAATCAGAAC CCGAGGGCGA CGCTGCCCGC GAGGTCCAGG CGCTGGCCGC TGAAATTAAA
     5641 TCAAAACTCA TTTGAGTTAA TGAGGTAAAG AGAAAATGAG CAAAAGCACA AACACGCTAA
     5701 GTGCCGGCCG TCCGAGCGCA CGCAGCAGCA AGGCTGCAAC GTTGGCCAGC CTGGCAGACA
     5761 CGCCAGCCAT GAAGCGGGTC AACTTTCAGT TGCCGGCGGA GGATCACACC AAGCTGAAGA
     5821 TGTACGCGGT ACGCCAAGGC AAGACCATTA CCGAGCTGCT ATCTGAATAC ATCGCGCAGC
     5881 TACCAGAGTA AATGAGCAAA TGAATAAATG AGTAGATGAA TTTTAGCGGC TAAAGGAGGC
     5941 GGCATGGAAA ATCAAGAACA ACCAGGCACC GACGCCGTGG AATGCCCCAT GTGTGGAGGA
     6001 ACGGGCGGTT GGCCAGGCGT AAGCGGCTGG GTTGTCTGCC GGCCCTGCAA TGGCACTGGA
     6061 ACCCCCAAGC CCGAGGAATC GGCGTGACGG TCGCAAACCA TCCGGCCCGG TACAAATCGG
     6121 CGCGGCGCTG GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA
     6181 ACGCATCGAG GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG
     6241 CAAAGAATCC CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG
     6301 CGACGAGCAA CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG
     6361 CAGCATCATG GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT
     6421 GATCCGCTAC GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC
     6481 CAGTGTGTGG GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA
     6541 CCGATACCGG GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA
     6601 CGTACTCAAG TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC
     6661 CTGCATTCGG TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG
     6721 CCGCCTGGTG ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG
     6781 CGAAACCGGG CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT
     6841 CACAGAAGGC AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC
     6901 CGGCATCGGC CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG
     6961 ATGGTTGTTC AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG
     7021 TTTCACCGTG CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA
     7081 GGCGGGGCAG GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC
     7141 ATCCGCCGGT TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA
     7201 AGGTCGAAAA AGCTTCTTTC CTGTGGATAG CACGTACATT GGGAACCCAA AGCCGTACAT
     7261 TGGGAACCGG AACCCGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGTCACACAT
     7321 GTAAGTGACT GATATAAAAG AGAAAAAAGG CGATTTTTCC GCCTAAAACT CTTTAAAACT
     7381 TATTAAAACT CTTAAAACCC GCCTGGCCTG TGCATAACTG TCTGGCCAGC GCACAGCCGA
     7441 ACAGCTGCAA AAAGCGCCCA CATCAAGGCT CCGAGTGCGC GGAACCCCTA TTTGTTTATT
     7501 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
     7561 ATAATATTGA AAAAGGAAGA GTATGGCTAA AATGAGAATA TCACCGGAAT TGAAAAAACT
     7621 GATCGAAAAA TACCGCTGCG TAAAAGATAC GGAAGGAATG TCTCCTGCTA AGGTATATAA
     7681 GCTGGTGGGA GAAAATGAAA ACCTATATTT AAAAATGACG GACAGCCGGT ATAAAGGGAC
     7741 CACCTATGAT GTGGAACGGG AAAAGGACAT GATGCTATGG CTGGAAGGAA AGCTGCCTGT
     7801 TCCAAAGGTC CTGCACTTTG AACGGCATGA TGGCTGGAGC AATCTGCTCA TGAGTGAGGC
     7861 CGATGGCGTC CTTTGCTCGG AAGAGTATGA AGATGAACAA AGCCCTGAAA AGATTATCGA
     7921 GCTGTATGCG GAGTGCATCA GGCTCTTTCA CTCCATCGAC ATATCGGATT GTCCCTATAC
     7981 GAATAGCTTA GACAGCCGCT TAGCCGAATT GGATTACTTA CTGAATAACG ATCTGGCCGA
     8041 TGTGGATTGC GAAAACTGGG AAGAGGACAC TCCATTTAAA GATCCGCGCG AGCTGTATGA
     8101 TTTTTTAAAG ACGGAAAAGC CCGAAGAGGA ACTTGTCTTT TCCCACGGCG ACCTGGGAGA
     8161 CAGCAACATC TTTGTGAAAG ATGGCAAAGT AAGTGGCTTT ATTGATCTTG GGAGAAGCGG
     8221 CAGGGCGGAC AAGTGGTATG ACATTGCCTT CTGCGTCCGG TCGCTCAGGG AGGATATCGG
     8281 GGAAGAACAG TATGTCGAGC TATTTTTTGA CTTACTGGGG ATCAAGCCTG ATTGGGAGAA
     8341 AATAAAATAT TATATTTTAC TGGATGAATT GTTTTAGCTG TCAGACCAAG TTTACTCATA
     8401 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     8461 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCTAGC TGTAACCTCG
     8521 AAGCGTTTCA CTTGTAACAA CGATTGAGAA TTTTGTCATA AAATTGAAAT ACTTGGTTCG
     8581 CATTTTCGTC ATCCGCGGTC AGCCGCAATT CTGACGAACT GCCCATTTAG CTGGAGATGA
     8641 TTGTACATCC TTCACGTGAA AATTTCTCAA GCGCTGTGAA CAAGGGTTCA GATTTTAGAT
     8701 TGAAAGGTGA GCCGTTGAAA CACGTTCTTC TTGTCGATGA CGACGTCGCT ATGCGGCATC
     8761 TTATTATTGA ATACCTTACG ATCCACGCCT TCAAAGTGAC CGCGGTAGCC GACAGCACCC
     8821 AGTTCACAAG AGTACTCTCT TCCGCGACGG TCGATGTCGT GGTTGTTGAT CTAGATTTAG
     8881 GTCGTGAAGA TGGGCTCGAG ATCGTTCGTA ATCTGGCGGC AAAGTCTGAT ATTCCAATCA
     8941 TAATTATCAG TGGCGACCGC CTTGAGGAGA CGGATAAAGT TGTTGCACTC GAGCTAGGAG
     9001 CAAGTGATTT TATCGCTAAG CCGTTCAGTA TCAGAGAGTT TCTAGCACGC ATTCGGGTTG
     9061 CCTTGCGCGT GCGCCCCAAC GTTGTCCGCT CCAAAGACCG ACGGTCTTTT TGTTTTACTG
     9121 ACTGGACACT TAATCTCAGG CAACGTCGCT TGATGTCCGA AGCTGGCGGT GAGGTGAAAC
     9181 TTACGGCAGG TGAGTTCAAT CTTCTCCTCG CGTTTTTAGA GAAACCCCGC GACGTTCTAT
     9241 CGCGCGAGCA ACTTCTCATT GCCAGTCGAG TACGCGACGA GGAGGTTTAT GACAGGAGTA
     9301 TAGATGTTCT CATTTTGAGG CTGCGCCGCA AACTTGAGGC GGATCCGTCA AGCCCTCAAC
     9361 TGATAAAAAC AGCAAGAGGT GCCGGTTATT TCTTTGACGC GGACGTGCAG GTTTCGCACG
     9421 GGGGGACGAT GGCAGCCTGA GCCAATTGCA TTTGCCTCTT AATTATCTGG CTCAAAGGGT
     9481 GACTGAGGAG TAAGCGATGT GCCCATCACA CTGACCACCA AGACGGGGGT TCGTTTTGAA
     9541 GATCACCCTT CAGACCCAAG GCCAGTGTGC GTTGATCGCG GCCACACTGC AAATTGACAT
     9601 ACTGTCGATA AAGGGGCGTT GGACGAGAAC CCATTCGTCA GGATACTGGC TCGTGATCTC
     9661 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
     9721 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
     9781 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
     9841 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
     9901 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
     9961 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
    10021 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
    10081 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
    10141 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT
    10201 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT
    10261 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
    10321 TGCTCGGATC TGTTGGACCG GACAGTAGTC ATGGTTGATG GGCTGCCTGT ATCGAGTGGT
    10381 GATTTTGTGC CGAGCTGCCG GTCGGGGAGC TGTTGGCTGG CTGGTGGCAG GATATATTGT
    10441 GGTGTAAACA AATTGACGCT TAGACAACTT AATAACACAT TGCGGACGTT TTTAATGTAC
    10501 TGGGGTTGAA CACTCT
//
