LOCUS       pSB117_-_pL2_pSB90_fLUC-I_V2     10348 bp    DNA     circular SYN 21-NOV-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAGM4723 cor
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2013 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Confirmed by HindIII and NdeI digest. 
FEATURES             Location/Qualifiers
     Vector          join(4519..10348,1)
                     /origin="pSB90 - pAGM4723_VirGN54D (NEBuilder) (14,072bp
                     circular)Target vector: from BbsI cut at 8096^8097 to
                     BbsI cut at 1^2, 5,977bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     misc_feature    2..20
                     /feature_type="Insertion"
                     /origin="pICH54011 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    21..1502
                     /feature_type="Insertion"
                     /origin="pSB69 - pL1F2_2x35S::V2::Tnos (5,834bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 5817^5818, 1,482bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(1503..>1506,<25..40))
                     /origin="pICH47742 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        41..793
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(41..883)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           810..871
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    880..1231
                     /feature_type="Insertion"
                     /origin="V2 (CDS1) (376bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 362^363, 352bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     source          <880..>1231
                     /organism="Tomato yellow leaf curl virus"
                     /mol_type="genomic DNA"
                     /isolate="Almeria"
                     /host="Capsicum anuum"
                     /db_xref="taxon:10832"
                     /country="Spain:Almeria"
     CDS             881..1231
                     /gene="v2"
                     /locus_tag="TYLCVgp1"
                     /function="movement"
                     /codon_start=1
                     /product="precoat protein"
                     /protein_id="NP_658991.1"
                     /db_xref="GeneID:949136"
                     /translation="MWDPLLNEFPESVHGFRCMLAIKYLQSVEETYEPNTLGHDLIRD
                     LISVVRARDYVEATRRYNHFHARLEGSPKAELRQPIQQPCCCPHCPRHKQATIMDVQA
                     HVPKAQNIQNVSKP"
                     /note="Esp3I site removed"
     gene            881..1231
                     /gene="v2"
                     /locus_tag="TYLCVgp1"
                     /db_xref="GeneID:949136"
     misc_feature    complement(884..1235)
                     /feature_type="Insertion"
                     /origin="pSB61 - pL0_V2 (CDS1) (2,599bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 2583^2584, 352bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(1236..1502)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      1236..1498
                     /vntifkey="43"
                     /label="nost"
     misc_feature    1503..1521
                     /feature_type="Insertion"
                     /origin="pICH54033 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    1522..4494
                     /feature_type="Insertion"
                     /origin="pSB72 - pL1F4_2x35S::fLUC-I::Tnos (7,325bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 7308^7309, 2,973bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(4495..>4498,<1526..1541))
                     /origin="pICH47761 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        1542..2294
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(1542..2384)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           2311..2372
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    2381..2525
                     /feature_type="Insertion"
                     /origin="fLuc part1 (169bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 155^156, 145bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             2382..2525
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(2385..>2529)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(2385..4227)
                     /feature_type="Insertion"
                     /origin="pSB63 - pL0_fLuc-I (CDS1) (4,090bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 4074^4075, 1,843bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          2526..2714
                     /note="U5 small nuclear ribonucleoprotein component
                     (Arabidopsis thaliana)"
     misc_feature    2526..2710
                     /feature_type="Insertion"
                     /origin="fLuc part2 (209bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 195^196, 185bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(<2530..>2714)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    2711..4223
                     /feature_type="Insertion"
                     /origin="fLuc part3 (1,537bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 1523^1524,
                     1,513bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             2715..4223
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(<2715..>4227)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(4228..4494)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      4228..4490
                     /vntifkey="43"
                     /label="nost"
     misc_feature    4495..4518
                     /feature_type="Insertion"
                     /origin="pICH41780 (3,318bp circular)Fragment: from BbsI
                     cut at 2847^2848 to BbsI cut at 2871^2872,
                     24bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    4499..4518
                     /vntifkey="21"
                     /label="EL4"
     misc_feature    complement(4536..4663)
                     /label="RB"
                     /note="RB"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    4668..7302
                     /label="pVS1 fragment short"
                     /note="pVS1 fragment short"
                     /note="color: #66cc99; direction: RIGHT"
     CDS             4858..5487
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 StaA"
     CDS             5921..6988
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 RepA"
     rep_origin      7054..7248
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     promoter        7310..7414
                     /label="AmpR promoter"
     CDS             7415..8206
                     /codon_start=1
                     /label="NPTII"
                     /note="NPTII"
                     /note="color: #ff9966"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       7897
                     /note="A to G (BpiI removed)"
                     /note="color: #3366cc"
     variation       8096
                     /note="A to C, I to L"
                     /note="color: #3366cc"
     misc_feature    8339..9491
                     /label="VirGN54D insert"
     CDS             8469..9272
                     /label="VirGN54D"
     misc_feature    9522..10136
                     /label="rep - pMB1"
                     /note="rep - pMB1"
                     /note="color: #66cc99; direction: RIGHT"
     variation       9680
                     /note="C to T"
                     /note="color: #3366cc"
     rep_origin      10121
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     misc_feature    complement(10188..10338)
                     /label="LB"
                     /note="LB"
                     /note="color: #66cc99; direction: LEFT"
ORIGIN
        1 GTGCCGATGT ATCACCTCTC GCAAGAATTC AAGCTTGGAG GTCAACATGG TGGAGCACGA
       61 CACTCTGGTC TACTCCAAAA ATGTCAAAGA TACAGTCTCA GAAGATCAAA GGGCTATTGA
      121 GACTTTTCAA CAAAGGATAA TTTCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG
      181 TCACTTCATC GAAAGGACAG TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA
      241 TAAAGGAAAG GCTATCATTC AAGATCTCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC
      301 ACCCACGAGG AGCATCGTGG AAAAAGAAGA GGTTCCAACC ACGTCTACAA AGCAAGTGGA
      361 TTGATGTGAT AACATGGTGG AGCACGACAC TCTGGTCTAC TCCAAAAATG TCAAAGATAC
      421 AGTCTCAGAA GATCAAAGGG CTATTGAGAC TTTTCAACAA AGGATAATTT CGGGAAACCT
      481 CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTCATCGAA AGGACAGTAG AAAAGGAAGG
      541 TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCATTCAAG ATCTCTCTGC
      601 CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGAGGT
      661 TCCAACCACG TCTACAAAGC AAGTGGATTG ATGTGACATC TCCACTGACG TAAGGGATGA
      721 CGCACAATCC CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT
      781 GGAGAGGACA CGCTCGAGTA TAAGAGCTCA TTTTTACAAC AATTACCAAC AACAACAAAC
      841 AACAAACAAC ATTACAATTA CATTTACAAT TATCGATACA ATGTGGGACC CACTTCTAAA
      901 TGAATTTCCT GAATCTGTTC ACGGATTTCG TTGTATGTTA GCTATTAAAT ATTTGCAGTC
      961 CGTTGAGGAA ACTTACGAGC CCAATACATT GGGCCACGAT TTAATTAGGG ATCTTATATC
     1021 TGTTGTAAGG GCCCGTGACT ATGTCGAAGC GACCAGGCGA TATAATCATT TCCACGCCAG
     1081 ACTCGAAGGT TCGCCGAAGG CTGAACTTCG ACAGCCCATA CAGCAGCCGT GCTGCTGTCC
     1141 CCATTGTCCA AGGCACAAAC AAGCGACGAT CATGGACGTA CAGGCCCATG TACCGAAAGC
     1201 CCAGAATATA CAGAATGTAT CGAAGCCCTG AGCTTGTCAA GCAGATCGTT CAAACATTTG
     1261 GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT
     1321 TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG
     1381 ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT
     1441 ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGACG
     1501 CTACTAGCAA CATACGCTGG ATTACGAATT CCCATGGGGA GGTCAACATG GTGGAGCACG
     1561 ACACTCTGGT CTACTCCAAA AATGTCAAAG ATACAGTCTC AGAAGATCAA AGGGCTATTG
     1621 AGACTTTTCA ACAAAGGATA ATTTCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT
     1681 GTCACTTCAT CGAAAGGACA GTAGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG
     1741 ATAAAGGAAA GGCTATCATT CAAGATCTCT CTGCCGACAG TGGTCCCAAA GATGGACCCC
     1801 CACCCACGAG GAGCATCGTG GAAAAAGAAG AGGTTCCAAC CACGTCTACA AAGCAAGTGG
     1861 ATTGATGTGA TAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA
     1921 CAGTCTCAGA AGATCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC
     1981 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG
     2041 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG
     2101 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGAGG
     2161 TTCCAACCAC GTCTACAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG
     2221 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT
     2281 TGGAGAGGAC ACGCTCGAGT ATAAGAGCTC ATTTTTACAA CAATTACCAA CAACAACAAA
     2341 CAACAAACAA CATTACAATT ACATTTACAA TTATCGATAC AATGGAGGAC GCCAAAAACA
     2401 TAAAGAAAGG CCCGGCGCCA TTCTATCCTC TAGAGGATGG AACCGCTGGA GAGCAACTGC
     2461 ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT TGCTTTTACA GATGCACATA
     2521 TCGAGGTAAG TTTCTGCTTC TACCTTTGAT ATATATATAA TAATTATCAT TAATTAGTAG
     2581 TAATATAATA TTTCAAATAT TTTTTTCAAA ATAAAAGAAT GTAGTATATA GCAATTGCTT
     2641 TTCTGTAGTT TATAAGTGTG TATATTTTAA TTTATAACTT TTCTAATATA TGACCAAAAT
     2701 TTGTTGATGT GCAGGTGAAC ATCACGTACG CGGAATACTT CGAAATGTCC GTTCGGTTGG
     2761 CAGAAGCTAT GAAACGATAT GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA
     2821 ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT TATCGGAGTT GCAGTTGCGC
     2881 CCGCGAACGA CATTTATAAT GAACGTGAAT TGCTCAACAG TATGAACATT TCGCAGCCTA
     2941 CCGTAGTGTT TGTTTCCAAA AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAATTAC
     3001 CAATAATCCA GAAAATTATT ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA
     3061 TGTACACGTT CGTCACATCT CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTACCAG
     3121 AGTCCTTTGA TCGTGACAAA ACAATTGCAC TGATAATGAA TTCCTCTGGA TCTACTGGGT
     3181 TACCTAAGGG TGTGGCCCTT CCGCATAGAA CTGCCTGCGT CAGATTCTCG CATGCCAGAG
     3241 ATCCTATTTT TGGCAATCAA ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC
     3301 ATCACGGTTT TGGAATGTTT ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT
     3361 TAATGTATAG ATTTGAAGAA GAGCTGTTTT TACGATCCCT TCAGGATTAC AAAATTCAAA
     3421 GTGCGTTGCT AGTACCAACC CTATTTTCAT TCTTCGCCAA AAGCACTCTG ATTGACAAAT
     3481 ACGATTTATC TAATTTACAC GAAATTGCTT CTGGGGGCGC ACCTCTTTCG AAAGAAGTCG
     3541 GGGAAGCGGT TGCAAAACGC TTCCATCTTC CAGGGATACG ACAAGGATAT GGGCTCACTG
     3601 AGACTACATC AGCTATTCTG ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA
     3661 AAGTTGTTCC ATTTTTTGAA GCGAAGGTTG TGGATCTGGA TACCGGGAAA ACGCTGGGCG
     3721 TTAATCAGAG AGGCGAATTA TGTGTCAGAG GACCTATGAT TATGTCCGGT TATGTAAACA
     3781 ATCCGGAAGC GACCAACGCC TTGATTGACA AGGATGGATG GCTACATTCT GGAGACATAG
     3841 CTTACTGGGA CGAGGACGAA CACTTCTTCA TAGTTGACCG CTTGAAGTCT TTAATTAAAT
     3901 ACAAAGGATA TCAGGTGGCC CCCGCTGAAT TGGAATCGAT ATTGTTACAA CACCCCAACA
     3961 TCTTCGACGC GGGCGTGGCA GGTCTGCCCG ACGATGACGC CGGTGAACTT CCCGCCGCCG
     4021 TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA GATCGTGGAT TACGTCGCCA
     4081 GTCAAGTAAC AACCGCGAAA AAGTTGCGCG GAGGAGTTGT GTTTGTGGAC GAAGTACCGA
     4141 AAGGTCTTAC CGGAAAACTC GACGCAAGAA AAATCAGAGA GATCCTCATA AAGGCCAAGA
     4201 AGGGCGGAAA GTCCAAATTG TAAGCTTGTC AAGCAGATCG TTCAAACATT TGGCAATAAA
     4261 GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA
     4321 ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT
     4381 TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG
     4441 CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGA CGCTCAGAGA
     4501 GGATGCACAT GTGACCGAGG GACACGAAGT GATCCGTTTA AACTATCAGT GTTTGACAGG
     4561 ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA
     4621 AGGGCGTGAA AAGGTTTATC CGTTCGTCCA TTTGTATGTG CCAGCCGTGC GGCTGCATGA
     4681 AATCCTGGCC GGTTTGTCTG ATGCCAAGCT GGCGGCCTGG CCGGCCAGCT TGGCCGCTGA
     4741 AGAAACCGAG CGCCGCCGTC TAAAAAGGTG ATGTGTATTT GAGTAAAACA GCTTGCGTCA
     4801 TGCGGTCGCT GCGTATATGA TGCGATGAGT AAATAAACAA ATACGCAAGG GGAACGCATG
     4861 AAGGTTATCG CTGTACTTAA CCAGAAAGGC GGGTCAGGCA AGACGACCAT CGCAACCCAT
     4921 CTAGCCCGCG CCCTGCAACT CGCCGGGGCC GATGTTCTGT TAGTCGATTC CGATCCCCAG
     4981 GGCAGTGCCC GCGATTGGGC GGCCGTGCGG GAAGATCAAC CGCTAACCGT TGTCGGCATC
     5041 GACCGCCCGA CGATTGACCG CGACGTGAAG GCCATCGGCC GGCGCGACTT CGTAGTGATC
     5101 GACGGAGCGC CCCAGGCGGC GGACTTGGCT GTGTCCGCGA TCAAGGCAGC CGACTTCGTG
     5161 CTGATTCCGG TGCAGCCAAG CCCTTACGAC ATATGGGCCA CCGCCGACCT GGTGGAGCTG
     5221 GTTAAGCAGC GCATTGAGGT CACGGATGGA AGGCTACAAG CGGCCTTTGT CGTGTCGCGG
     5281 GCGATCAAAG GCACGCGCAT CGGCGGTGAG GTTGCCGAGG CGCTGGCCGG GTACGAGCTG
     5341 CCCATTCTTG AGTCCCGTAT CACGCAGCGC GTGAGCTACC CAGGCACTGC CGCCGCCGGC
     5401 ACAACCGTTC TTGAATCAGA ACCCGAGGGC GACGCTGCCC GCGAGGTCCA GGCGCTGGCC
     5461 GCTGAAATTA AATCAAAACT CATTTGAGTT AATGAGGTAA AGAGAAAATG AGCAAAAGCA
     5521 CAAACACGCT AAGTGCCGGC CGTCCGAGCG CACGCAGCAG CAAGGCTGCA ACGTTGGCCA
     5581 GCCTGGCAGA CACGCCAGCC ATGAAGCGGG TCAACTTTCA GTTGCCGGCG GAGGATCACA
     5641 CCAAGCTGAA GATGTACGCG GTACGCCAAG GCAAGACCAT TACCGAGCTG CTATCTGAAT
     5701 ACATCGCGCA GCTACCAGAG TAAATGAGCA AATGAATAAA TGAGTAGATG AATTTTAGCG
     5761 GCTAAAGGAG GCGGCATGGA AAATCAAGAA CAACCAGGCA CCGACGCCGT GGAATGCCCC
     5821 ATGTGTGGAG GAACGGGCGG TTGGCCAGGC GTAAGCGGCT GGGTTGTCTG CCGGCCCTGC
     5881 AATGGCACTG GAACCCCCAA GCCCGAGGAA TCGGCGTGAC GGTCGCAAAC CATCCGGCCC
     5941 GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG AAGTTGAAGG CCGCGCAGGC
     6001 CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT GAATCGTGGC AAGCGGCCGC
     6061 TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC GGTGCGCCGT CGATTAGGAA
     6121 GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG ATGCTCTATG ACGTGGGCAC
     6181 CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT CTGTCGAAGC GTGACCGACG
     6241 AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC GTAGAGGTTT CCGCAGGGCC
     6301 GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG ATGGCGGTTT CCCATCTAAC
     6361 CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG CCCGGCCGCG TGTTCCGTCC
     6421 ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT GGCGGAAAGC AGAAAGACGA
     6481 CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT GCCATGCAGC GTACGAAGAA
     6541 GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA GCCTTGATTA GCCGCTACAA
     6601 GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG ATCGAGCTAG CTGATTGGAT
     6661 GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG ACGGTTCACC CCGATTACTT
     6721 TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG GCACGCCGCG CCGCAGGCAA
     6781 GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC AGTGGCAGCG CCGGAGAGTT
     6841 CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA AATGACCTGC CGGAGTACGA
     6901 TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC ATGCGCTACC GCAACCTGAT
     6961 CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG ATGCTAGGGC AAATTGCCCT
     7021 AGCAGGGGAA AAAGGTCGAA AAAGCTTCTT TCCTGTGGAT AGCACGTACA TTGGGAACCC
     7081 AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA
     7141 CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA GGCGATTTTT CCGCCTAAAA
     7201 CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC TGTGCATAAC TGTCTGGCCA
     7261 GCGCACAGCC GAACAGCTGC AAAAAGCGCC CACATCAAGG CTCCGAGTGC GCGGAACCCC
     7321 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     7381 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGGCT AAAATGAGAA TATCACCGGA
     7441 ATTGAAAAAA CTGATCGAAA AATACCGCTG CGTAAAAGAT ACGGAAGGAA TGTCTCCTGC
     7501 TAAGGTATAT AAGCTGGTGG GAGAAAATGA AAACCTATAT TTAAAAATGA CGGACAGCCG
     7561 GTATAAAGGG ACCACCTATG ATGTGGAACG GGAAAAGGAC ATGATGCTAT GGCTGGAAGG
     7621 AAAGCTGCCT GTTCCAAAGG TCCTGCACTT TGAACGGCAT GATGGCTGGA GCAATCTGCT
     7681 CATGAGTGAG GCCGATGGCG TCCTTTGCTC GGAAGAGTAT GAAGATGAAC AAAGCCCTGA
     7741 AAAGATTATC GAGCTGTATG CGGAGTGCAT CAGGCTCTTT CACTCCATCG ACATATCGGA
     7801 TTGTCCCTAT ACGAATAGCT TAGACAGCCG CTTAGCCGAA TTGGATTACT TACTGAATAA
     7861 CGATCTGGCC GATGTGGATT GCGAAAACTG GGAAGAGGAC ACTCCATTTA AAGATCCGCG
     7921 CGAGCTGTAT GATTTTTTAA AGACGGAAAA GCCCGAAGAG GAACTTGTCT TTTCCCACGG
     7981 CGACCTGGGA GACAGCAACA TCTTTGTGAA AGATGGCAAA GTAAGTGGCT TTATTGATCT
     8041 TGGGAGAAGC GGCAGGGCGG ACAAGTGGTA TGACATTGCC TTCTGCGTCC GGTCGCTCAG
     8101 GGAGGATATC GGGGAAGAAC AGTATGTCGA GCTATTTTTT GACTTACTGG GGATCAAGCC
     8161 TGATTGGGAG AAAATAAAAT ATTATATTTT ACTGGATGAA TTGTTTTAGC TGTCAGACCA
     8221 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
     8281 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCTA
     8341 GCTGTAACCT CGAAGCGTTT CACTTGTAAC AACGATTGAG AATTTTGTCA TAAAATTGAA
     8401 ATACTTGGTT CGCATTTTCG TCATCCGCGG TCAGCCGCAA TTCTGACGAA CTGCCCATTT
     8461 AGCTGGAGAT GATTGTACAT CCTTCACGTG AAAATTTCTC AAGCGCTGTG AACAAGGGTT
     8521 CAGATTTTAG ATTGAAAGGT GAGCCGTTGA AACACGTTCT TCTTGTCGAT GACGACGTCG
     8581 CTATGCGGCA TCTTATTATT GAATACCTTA CGATCCACGC CTTCAAAGTG ACCGCGGTAG
     8641 CCGACAGCAC CCAGTTCACA AGAGTACTCT CTTCCGCGAC GGTCGATGTC GTGGTTGTTG
     8701 ATCTAGATTT AGGTCGTGAA GATGGGCTCG AGATCGTTCG TAATCTGGCG GCAAAGTCTG
     8761 ATATTCCAAT CATAATTATC AGTGGCGACC GCCTTGAGGA GACGGATAAA GTTGTTGCAC
     8821 TCGAGCTAGG AGCAAGTGAT TTTATCGCTA AGCCGTTCAG TATCAGAGAG TTTCTAGCAC
     8881 GCATTCGGGT TGCCTTGCGC GTGCGCCCCA ACGTTGTCCG CTCCAAAGAC CGACGGTCTT
     8941 TTTGTTTTAC TGACTGGACA CTTAATCTCA GGCAACGTCG CTTGATGTCC GAAGCTGGCG
     9001 GTGAGGTGAA ACTTACGGCA GGTGAGTTCA ATCTTCTCCT CGCGTTTTTA GAGAAACCCC
     9061 GCGACGTTCT ATCGCGCGAG CAACTTCTCA TTGCCAGTCG AGTACGCGAC GAGGAGGTTT
     9121 ATGACAGGAG TATAGATGTT CTCATTTTGA GGCTGCGCCG CAAACTTGAG GCGGATCCGT
     9181 CAAGCCCTCA ACTGATAAAA ACAGCAAGAG GTGCCGGTTA TTTCTTTGAC GCGGACGTGC
     9241 AGGTTTCGCA CGGGGGGACG ATGGCAGCCT GAGCCAATTG CATTTGCCTC TTAATTATCT
     9301 GGCTCAAAGG GTGACTGAGG AGTAAGCGAT GTGCCCATCA CACTGACCAC CAAGACGGGG
     9361 GTTCGTTTTG AAGATCACCC TTCAGACCCA AGGCCAGTGT GCGTTGATCG CGGCCACACT
     9421 GCAAATTGAC ATACTGTCGA TAAAGGGGCG TTGGACGAGA ACCCATTCGT CAGGATACTG
     9481 GCTCGTGATC TCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     9541 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     9601 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     9661 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     9721 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     9781 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     9841 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     9901 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     9961 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
    10021 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
    10081 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
    10141 TTTTACGGTT CCTGCTCGGA TCTGTTGGAC CGGACAGTAG TCATGGTTGA TGGGCTGCCT
    10201 GTATCGAGTG GTGATTTTGT GCCGAGCTGC CGGTCGGGGA GCTGTTGGCT GGCTGGTGGC
    10261 AGGATATATT GTGGTGTAAA CAAATTGACG CTTAGACAAC TTAATAACAC ATTGCGGACG
    10321 TTTTTAATGT ACTGGGGTTG AACACTCT
//
