LOCUS       pSB118_-_pL2_pSB90_fLUC-I_HcPro     11380 bp    DNA     circular SYN 21-NOV-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAGM4723 cor
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2013 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Confirmed by NdeI digest. 
            
FEATURES             Location/Qualifiers
     Vector          join(5551..11380,1)
                     /origin="pSB90 - pAGM4723_VirGN54D (NEBuilder) (14,072bp
                     circular)Target vector: from BbsI cut at 8096^8097 to
                     BbsI cut at 1^2, 5,977bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     misc_feature    2..20
                     /feature_type="Insertion"
                     /origin="pICH54011 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    21..39
                     /feature_type="Insertion"
                     /origin="pICH54022 (4,368bp circular)Fragment: from BbsI
                     cut at 4349^4350 to BbsI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    40..2553
                     /feature_type="Insertion"
                     /origin="pSB70 - pL1F3_2x35S::HcPro::Tnos (6,866bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 6849^6850, 2,514bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(2554..>2557,<44..59))
                     /origin="pICH47751 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        60..812
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(60..902)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           829..890
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     CDS             900..2282
                     /label="TEV-HcPro"
     misc_feature    complement(903..2286)
                     /feature_type="Insertion"
                     /origin="pSB64 - pHcPro (CDS1) (4,441bp
                     circular)Fragment: from Eco31I cut at 4^5 to Eco31I cut
                     at 1388^1389, 1,384bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(2287..2553)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      2287..2549
                     /vntifkey="43"
                     /label="nost"
     misc_feature    2554..5526
                     /feature_type="Insertion"
                     /origin="pSB72 - pL1F4_2x35S::fLUC-I::Tnos (7,325bp
                     circular)Fragment: from BbsI cut at 4335^4336 to BbsI cut
                     at 7308^7309, 2,973bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(5527..>5530,<2558..2573))
                     /origin="pICH47761 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        2574..3326
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(2574..3416)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           3343..3404
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    3413..3557
                     /feature_type="Insertion"
                     /origin="fLuc part1 (169bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 155^156, 145bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             3414..3557
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(3417..>3561)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(3417..5259)
                     /feature_type="Insertion"
                     /origin="pSB63 - pL0_fLuc-I (CDS1) (4,090bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 4074^4075, 1,843bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          3558..3746
                     /note="U5 small nuclear ribonucleoprotein component
                     (Arabidopsis thaliana)"
     misc_feature    3558..3742
                     /feature_type="Insertion"
                     /origin="fLuc part2 (209bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 195^196, 185bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(<3562..>3746)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    3743..5255
                     /feature_type="Insertion"
                     /origin="fLuc part3 (1,537bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 1523^1524,
                     1,513bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             3747..5255
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLuc-I"
     misc_feature    complement(<3747..>5259)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(5260..5526)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      5260..5522
                     /vntifkey="43"
                     /label="nost"
     misc_feature    5527..5550
                     /feature_type="Insertion"
                     /origin="pICH41780 (3,318bp circular)Fragment: from BbsI
                     cut at 2847^2848 to BbsI cut at 2871^2872,
                     24bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    5531..5550
                     /vntifkey="21"
                     /label="EL4"
     misc_feature    complement(5568..5695)
                     /label="RB"
                     /note="RB"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    5700..8334
                     /label="pVS1 fragment short"
                     /note="pVS1 fragment short"
                     /note="color: #66cc99; direction: RIGHT"
     CDS             5890..6519
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 StaA"
     CDS             6953..8020
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 RepA"
     rep_origin      8086..8280
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     promoter        8342..8446
                     /label="AmpR promoter"
     CDS             8447..9238
                     /codon_start=1
                     /label="NPTII"
                     /note="NPTII"
                     /note="color: #ff9966"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       8929
                     /note="A to G (BpiI removed)"
                     /note="color: #3366cc"
     variation       9128
                     /note="A to C, I to L"
                     /note="color: #3366cc"
     misc_feature    9371..10523
                     /label="VirGN54D insert"
     CDS             9501..10304
                     /label="VirGN54D"
     misc_feature    10554..11168
                     /label="rep - pMB1"
                     /note="rep - pMB1"
                     /note="color: #66cc99; direction: RIGHT"
     variation       10712
                     /note="C to T"
                     /note="color: #3366cc"
     rep_origin      11153
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     misc_feature    complement(11220..11370)
                     /label="LB"
                     /note="LB"
                     /note="color: #66cc99; direction: LEFT"
ORIGIN
        1 GTGCCGATGT ATCACCTCTC GCAAGGTTTT GACGGGTTTA CTAGAATTCG AGCTCGGAGG
       61 TCAACATGGT GGAGCACGAC ACTCTGGTCT ACTCCAAAAA TGTCAAAGAT ACAGTCTCAG
      121 AAGATCAAAG GGCTATTGAG ACTTTTCAAC AAAGGATAAT TTCGGGAAAC CTCCTCGGAT
      181 TCCATTGCCC AGCTATCTGT CACTTCATCG AAAGGACAGT AGAAAAGGAA GGTGGCTCCT
      241 ACAAATGCCA TCATTGCGAT AAAGGAAAGG CTATCATTCA AGATCTCTCT GCCGACAGTG
      301 GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAG GTTCCAACCA
      361 CGTCTACAAA GCAAGTGGAT TGATGTGATA ACATGGTGGA GCACGACACT CTGGTCTACT
      421 CCAAAAATGT CAAAGATACA GTCTCAGAAG ATCAAAGGGC TATTGAGACT TTTCAACAAA
      481 GGATAATTTC GGGAAACCTC CTCGGATTCC ATTGCCCAGC TATCTGTCAC TTCATCGAAA
      541 GGACAGTAGA AAAGGAAGGT GGCTCCTACA AATGCCATCA TTGCGATAAA GGAAAGGCTA
      601 TCATTCAAGA TCTCTCTGCC GACAGTGGTC CCAAAGATGG ACCCCCACCC ACGAGGAGCA
      661 TCGTGGAAAA AGAAGAGGTT CCAACCACGT CTACAAAGCA AGTGGATTGA TGTGACATCT
      721 CCACTGACGT AAGGGATGAC GCACAATCCC ACTATCCTTC GCAAGACCCT TCCTCTATAT
      781 AAGGAAGTTC ATTTCATTTG GAGAGGACAC GCTCGAGTAT AAGAGCTCAT TTTTACAACA
      841 ATTACCAACA ACAACAAACA ACAAACAACA TTACAATTAC ATTTACAATT ATCGATACAA
      901 TGagcgacaa atcaatctct gaggcattct tcataccata ctctaagaaa ttcttggagt
      961 taagaccaga tggaatttcc catgagtgta caagaggagt atcagttgag cggtgcggtg
     1021 aggtggctgc aatcctgaca caagcacttt caccgtgtgg taagatcaca tgcaaacgtt
     1081 gcatggttga aacacctgac attgttgagg gtgagtcggg agacagtgtc accaaccaag
     1141 gtaagctcct agcaatgctg aaagaacagt atccagattt cccaatggcc gagaaactac
     1201 tcacaaggtt tttgcaacag aaatcactag taaatacaaa tttgacagcc tgcgtgagcg
     1261 tcaaacaact cattggtgac cgcaaacaag ctccattcac acacgtactg gctgtcagcg
     1321 aaattctgtt taaaggcaat aaactaacag gggccgatct cgaagaggca agcacacata
     1381 tgcttgaaat agcaaggttc ttgaacaatc gcactgaaaa tatgcgcatt ggccaccttg
     1441 gttctttcag aaataaaatc tcatcgaagg cccatgtgaa taacgcactc atgtgtgata
     1501 atcaacttga tcagaatggg aattttattt ggggactaag gggtgcacac gcaaagaggt
     1561 ttcttaaagg atttttcact gagattgacc caaatgaagg atacgataag tatgttatca
     1621 ggaaacatat caggggtagc agaaagctag caattggcaa tttgataatg tcaactgact
     1681 tccagacgct caggcaacaa attcaaggcg aaactattga gcgtaaagaa attgggaatc
     1741 actgcatttc aatgcggaat ggtaattacg tgtacccatg ttgttgtgtt actcttgaag
     1801 atggtaaggc tcaatattcg gatctaaagc atccaacgaa gagacatctg gtcattggca
     1861 actctggcga ttcaaagtac ctagaccttc cagttctcaa tgaagagaaa atgtatatag
     1921 ctaatgaagg ttattgctac atgaacattt tctttgctct actagtgaat gtcaaggaag
     1981 aggatgcaaa ggacttcacc aagtttataa gggacacaat tgttccaaag cttggagcgt
     2041 ggccaacaat gcaagatgtt gcaactgcat gctacttact ttccattctt tacccagatg
     2101 tcctgagtgc tgaattaccc agaattttgg ttgatcatga caacaaaaca atgcatgttt
     2161 tggattcgta tgggtctaga acgacaggat accacatgtt gaaaatgaac acaacatccc
     2221 agctaattga attcgttcat tcaggtttgg aatccgaaat gaaaactttt aatgttggat
     2281 GAGCTTGTCA AGCAGATCGT TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG
     2341 TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA TTACGTTAAG CATGTAATAA
     2401 TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT TATGATTAGA GTCCCGCAAT
     2461 TATACATTTA ATACGCGATA GAAAACAAAA TATAGCGCGC AAACTAGGAT AAATTATCGC
     2521 GCGCGGTGTC ATCTATGTTA CTAGATCGAC GCTTTACGAA TTCCCATGGG GAGGTCAACA
     2581 TGGTGGAGCA CGACACTCTG GTCTACTCCA AAAATGTCAA AGATACAGTC TCAGAAGATC
     2641 AAAGGGCTAT TGAGACTTTT CAACAAAGGA TAATTTCGGG AAACCTCCTC GGATTCCATT
     2701 GCCCAGCTAT CTGTCACTTC ATCGAAAGGA CAGTAGAAAA GGAAGGTGGC TCCTACAAAT
     2761 GCCATCATTG CGATAAAGGA AAGGCTATCA TTCAAGATCT CTCTGCCGAC AGTGGTCCCA
     2821 AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGAGGTTCCA ACCACGTCTA
     2881 CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACTCTGGTC TACTCCAAAA
     2941 ATGTCAAAGA TACAGTCTCA GAAGATCAAA GGGCTATTGA GACTTTTCAA CAAAGGATAA
     3001 TTTCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTCATC GAAAGGACAG
     3061 TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCATTC
     3121 AAGATCTCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG
     3181 AAAAAGAAGA GGTTCCAACC ACGTCTACAA AGCAAGTGGA TTGATGTGAC ATCTCCACTG
     3241 ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA
     3301 GTTCATTTCA TTTGGAGAGG ACACGCTCGA GTATAAGAGC TCATTTTTAC AACAATTACC
     3361 AACAACAACA AACAACAAAC AACATTACAA TTACATTTAC AATTATCGAT ACAATGGAGG
     3421 ACGCCAAAAA CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG
     3481 GAGAGCAACT GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA
     3541 CAGATGCACA TATCGAGGTA AGTTTCTGCT TCTACCTTTG ATATATATAT AATAATTATC
     3601 ATTAATTAGT AGTAATATAA TATTTCAAAT ATTTTTTTCA AAATAAAAGA ATGTAGTATA
     3661 TAGCAATTGC TTTTCTGTAG TTTATAAGTG TGTATATTTT AATTTATAAC TTTTCTAATA
     3721 TATGACCAAA ATTTGTTGAT GTGCAGGTGA ACATCACGTA CGCGGAATAC TTCGAAATGT
     3781 CCGTTCGGTT GGCAGAAGCT ATGAAACGAT ATGGGCTGAA TACAAATCAC AGAATCGTCG
     3841 TATGCAGTGA AAACTCTCTT CAATTCTTTA TGCCGGTGTT GGGCGCGTTA TTTATCGGAG
     3901 TTGCAGTTGC GCCCGCGAAC GACATTTATA ATGAACGTGA ATTGCTCAAC AGTATGAACA
     3961 TTTCGCAGCC TACCGTAGTG TTTGTTTCCA AAAAGGGGTT GCAAAAAATT TTGAACGTGC
     4021 AAAAAAAATT ACCAATAATC CAGAAAATTA TTATCATGGA TTCTAAAACG GATTACCAGG
     4081 GATTTCAGTC GATGTACACG TTCGTCACAT CTCATCTACC TCCCGGTTTT AATGAATACG
     4141 ATTTTGTACC AGAGTCCTTT GATCGTGACA AAACAATTGC ACTGATAATG AATTCCTCTG
     4201 GATCTACTGG GTTACCTAAG GGTGTGGCCC TTCCGCATAG AACTGCCTGC GTCAGATTCT
     4261 CGCATGCCAG AGATCCTATT TTTGGCAATC AAATCATTCC GGATACTGCG ATTTTAAGTG
     4321 TTGTTCCATT CCATCACGGT TTTGGAATGT TTACTACACT CGGATATTTG ATATGTGGAT
     4381 TTCGAGTCGT CTTAATGTAT AGATTTGAAG AAGAGCTGTT TTTACGATCC CTTCAGGATT
     4441 ACAAAATTCA AAGTGCGTTG CTAGTACCAA CCCTATTTTC ATTCTTCGCC AAAAGCACTC
     4501 TGATTGACAA ATACGATTTA TCTAATTTAC ACGAAATTGC TTCTGGGGGC GCACCTCTTT
     4561 CGAAAGAAGT CGGGGAAGCG GTTGCAAAAC GCTTCCATCT TCCAGGGATA CGACAAGGAT
     4621 ATGGGCTCAC TGAGACTACA TCAGCTATTC TGATTACACC CGAGGGGGAT GATAAACCGG
     4681 GCGCGGTCGG TAAAGTTGTT CCATTTTTTG AAGCGAAGGT TGTGGATCTG GATACCGGGA
     4741 AAACGCTGGG CGTTAATCAG AGAGGCGAAT TATGTGTCAG AGGACCTATG ATTATGTCCG
     4801 GTTATGTAAA CAATCCGGAA GCGACCAACG CCTTGATTGA CAAGGATGGA TGGCTACATT
     4861 CTGGAGACAT AGCTTACTGG GACGAGGACG AACACTTCTT CATAGTTGAC CGCTTGAAGT
     4921 CTTTAATTAA ATACAAAGGA TATCAGGTGG CCCCCGCTGA ATTGGAATCG ATATTGTTAC
     4981 AACACCCCAA CATCTTCGAC GCGGGCGTGG CAGGTCTGCC CGACGATGAC GCCGGTGAAC
     5041 TTCCCGCCGC CGTTGTTGTT TTGGAGCACG GAAAGACGAT GACGGAAAAA GAGATCGTGG
     5101 ATTACGTCGC CAGTCAAGTA ACAACCGCGA AAAAGTTGCG CGGAGGAGTT GTGTTTGTGG
     5161 ACGAAGTACC GAAAGGTCTT ACCGGAAAAC TCGACGCAAG AAAAATCAGA GAGATCCTCA
     5221 TAAAGGCCAA GAAGGGCGGA AAGTCCAAAT TGTAAGCTTG TCAAGCAGAT CGTTCAAACA
     5281 TTTGGCAATA AAGTTTCTTA AGATTGAATC CTGTTGCCGG TCTTGCGATG ATTATCATAT
     5341 AATTTCTGTT GAATTACGTT AAGCATGTAA TAATTAACAT GTAATGCATG ACGTTATTTA
     5401 TGAGATGGGT TTTTATGATT AGAGTCCCGC AATTATACAT TTAATACGCG ATAGAAAACA
     5461 AAATATAGCG CGCAAACTAG GATAAATTAT CGCGCGCGGT GTCATCTATG TTACTAGATC
     5521 GACGCTCAGA GAGGATGCAC ATGTGACCGA GGGACACGAA GTGATCCGTT TAAACTATCA
     5581 GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAT
     5641 CGGATATTTA AAAGGGCGTG AAAAGGTTTA TCCGTTCGTC CATTTGTATG TGCCAGCCGT
     5701 GCGGCTGCAT GAAATCCTGG CCGGTTTGTC TGATGCCAAG CTGGCGGCCT GGCCGGCCAG
     5761 CTTGGCCGCT GAAGAAACCG AGCGCCGCCG TCTAAAAAGG TGATGTGTAT TTGAGTAAAA
     5821 CAGCTTGCGT CATGCGGTCG CTGCGTATAT GATGCGATGA GTAAATAAAC AAATACGCAA
     5881 GGGGAACGCA TGAAGGTTAT CGCTGTACTT AACCAGAAAG GCGGGTCAGG CAAGACGACC
     5941 ATCGCAACCC ATCTAGCCCG CGCCCTGCAA CTCGCCGGGG CCGATGTTCT GTTAGTCGAT
     6001 TCCGATCCCC AGGGCAGTGC CCGCGATTGG GCGGCCGTGC GGGAAGATCA ACCGCTAACC
     6061 GTTGTCGGCA TCGACCGCCC GACGATTGAC CGCGACGTGA AGGCCATCGG CCGGCGCGAC
     6121 TTCGTAGTGA TCGACGGAGC GCCCCAGGCG GCGGACTTGG CTGTGTCCGC GATCAAGGCA
     6181 GCCGACTTCG TGCTGATTCC GGTGCAGCCA AGCCCTTACG ACATATGGGC CACCGCCGAC
     6241 CTGGTGGAGC TGGTTAAGCA GCGCATTGAG GTCACGGATG GAAGGCTACA AGCGGCCTTT
     6301 GTCGTGTCGC GGGCGATCAA AGGCACGCGC ATCGGCGGTG AGGTTGCCGA GGCGCTGGCC
     6361 GGGTACGAGC TGCCCATTCT TGAGTCCCGT ATCACGCAGC GCGTGAGCTA CCCAGGCACT
     6421 GCCGCCGCCG GCACAACCGT TCTTGAATCA GAACCCGAGG GCGACGCTGC CCGCGAGGTC
     6481 CAGGCGCTGG CCGCTGAAAT TAAATCAAAA CTCATTTGAG TTAATGAGGT AAAGAGAAAA
     6541 TGAGCAAAAG CACAAACACG CTAAGTGCCG GCCGTCCGAG CGCACGCAGC AGCAAGGCTG
     6601 CAACGTTGGC CAGCCTGGCA GACACGCCAG CCATGAAGCG GGTCAACTTT CAGTTGCCGG
     6661 CGGAGGATCA CACCAAGCTG AAGATGTACG CGGTACGCCA AGGCAAGACC ATTACCGAGC
     6721 TGCTATCTGA ATACATCGCG CAGCTACCAG AGTAAATGAG CAAATGAATA AATGAGTAGA
     6781 TGAATTTTAG CGGCTAAAGG AGGCGGCATG GAAAATCAAG AACAACCAGG CACCGACGCC
     6841 GTGGAATGCC CCATGTGTGG AGGAACGGGC GGTTGGCCAG GCGTAAGCGG CTGGGTTGTC
     6901 TGCCGGCCCT GCAATGGCAC TGGAACCCCC AAGCCCGAGG AATCGGCGTG ACGGTCGCAA
     6961 ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG AGAAGTTGAA
     7021 GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG GTGAATCGTG
     7081 GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG CCGGTGCGCC
     7141 GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC CGATGCTCTA
     7201 TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC GTCTGTCGAA
     7261 GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC ACGTAGAGGT
     7321 TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC TGATGGCGGT
     7381 TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA AGCCCGGCCG
     7441 CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG ATGGCGGAAA
     7501 GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG TTGCCATGCA
     7561 GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG AAGCCTTGAT
     7621 TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG AGATCGAGCT
     7681 AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC TGACGGTTCA
     7741 CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC TGGCACGCCG
     7801 CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC GCAGTGGCAG
     7861 CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT CAAATGACCT
     7921 GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG TCATGCGCTA
     7981 CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC AGATGCTAGG
     8041 GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAAGCTTC TTTCCTGTGG ATAGCACGTA
     8101 CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC
     8161 GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT
     8221 TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA
     8281 ACTGTCTGGC CAGCGCACAG CCGAACAGCT GCAAAAAGCG CCCACATCAA GGCTCCGAGT
     8341 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     8401 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGG CTAAAATGAG
     8461 AATATCACCG GAATTGAAAA AACTGATCGA AAAATACCGC TGCGTAAAAG ATACGGAAGG
     8521 AATGTCTCCT GCTAAGGTAT ATAAGCTGGT GGGAGAAAAT GAAAACCTAT ATTTAAAAAT
     8581 GACGGACAGC CGGTATAAAG GGACCACCTA TGATGTGGAA CGGGAAAAGG ACATGATGCT
     8641 ATGGCTGGAA GGAAAGCTGC CTGTTCCAAA GGTCCTGCAC TTTGAACGGC ATGATGGCTG
     8701 GAGCAATCTG CTCATGAGTG AGGCCGATGG CGTCCTTTGC TCGGAAGAGT ATGAAGATGA
     8761 ACAAAGCCCT GAAAAGATTA TCGAGCTGTA TGCGGAGTGC ATCAGGCTCT TTCACTCCAT
     8821 CGACATATCG GATTGTCCCT ATACGAATAG CTTAGACAGC CGCTTAGCCG AATTGGATTA
     8881 CTTACTGAAT AACGATCTGG CCGATGTGGA TTGCGAAAAC TGGGAAGAGG ACACTCCATT
     8941 TAAAGATCCG CGCGAGCTGT ATGATTTTTT AAAGACGGAA AAGCCCGAAG AGGAACTTGT
     9001 CTTTTCCCAC GGCGACCTGG GAGACAGCAA CATCTTTGTG AAAGATGGCA AAGTAAGTGG
     9061 CTTTATTGAT CTTGGGAGAA GCGGCAGGGC GGACAAGTGG TATGACATTG CCTTCTGCGT
     9121 CCGGTCGCTC AGGGAGGATA TCGGGGAAGA ACAGTATGTC GAGCTATTTT TTGACTTACT
     9181 GGGGATCAAG CCTGATTGGG AGAAAATAAA ATATTATATT TTACTGGATG AATTGTTTTA
     9241 GCTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     9301 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     9361 GTTTTCGTTC TAGCTGTAAC CTCGAAGCGT TTCACTTGTA ACAACGATTG AGAATTTTGT
     9421 CATAAAATTG AAATACTTGG TTCGCATTTT CGTCATCCGC GGTCAGCCGC AATTCTGACG
     9481 AACTGCCCAT TTAGCTGGAG ATGATTGTAC ATCCTTCACG TGAAAATTTC TCAAGCGCTG
     9541 TGAACAAGGG TTCAGATTTT AGATTGAAAG GTGAGCCGTT GAAACACGTT CTTCTTGTCG
     9601 ATGACGACGT CGCTATGCGG CATCTTATTA TTGAATACCT TACGATCCAC GCCTTCAAAG
     9661 TGACCGCGGT AGCCGACAGC ACCCAGTTCA CAAGAGTACT CTCTTCCGCG ACGGTCGATG
     9721 TCGTGGTTGT TGATCTAGAT TTAGGTCGTG AAGATGGGCT CGAGATCGTT CGTAATCTGG
     9781 CGGCAAAGTC TGATATTCCA ATCATAATTA TCAGTGGCGA CCGCCTTGAG GAGACGGATA
     9841 AAGTTGTTGC ACTCGAGCTA GGAGCAAGTG ATTTTATCGC TAAGCCGTTC AGTATCAGAG
     9901 AGTTTCTAGC ACGCATTCGG GTTGCCTTGC GCGTGCGCCC CAACGTTGTC CGCTCCAAAG
     9961 ACCGACGGTC TTTTTGTTTT ACTGACTGGA CACTTAATCT CAGGCAACGT CGCTTGATGT
    10021 CCGAAGCTGG CGGTGAGGTG AAACTTACGG CAGGTGAGTT CAATCTTCTC CTCGCGTTTT
    10081 TAGAGAAACC CCGCGACGTT CTATCGCGCG AGCAACTTCT CATTGCCAGT CGAGTACGCG
    10141 ACGAGGAGGT TTATGACAGG AGTATAGATG TTCTCATTTT GAGGCTGCGC CGCAAACTTG
    10201 AGGCGGATCC GTCAAGCCCT CAACTGATAA AAACAGCAAG AGGTGCCGGT TATTTCTTTG
    10261 ACGCGGACGT GCAGGTTTCG CACGGGGGGA CGATGGCAGC CTGAGCCAAT TGCATTTGCC
    10321 TCTTAATTAT CTGGCTCAAA GGGTGACTGA GGAGTAAGCG ATGTGCCCAT CACACTGACC
    10381 ACCAAGACGG GGGTTCGTTT TGAAGATCAC CCTTCAGACC CAAGGCCAGT GTGCGTTGAT
    10441 CGCGGCCACA CTGCAAATTG ACATACTGTC GATAAAGGGG CGTTGGACGA GAACCCATTC
    10501 GTCAGGATAC TGGCTCGTGA TCTCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
    10561 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
    10621 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
    10681 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC
    10741 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
    10801 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
    10861 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
    10921 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
    10981 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
    11041 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
    11101 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
    11161 GCAACGCGGC CTTTTTACGG TTCCTGCTCG GATCTGTTGG ACCGGACAGT AGTCATGGTT
    11221 GATGGGCTGC CTGTATCGAG TGGTGATTTT GTGCCGAGCT GCCGGTCGGG GAGCTGTTGG
    11281 CTGGCTGGTG GCAGGATATA TTGTGGTGTA AACAAATTGA CGCTTAGACA ACTTAATAAC
    11341 ACATTGCGGA CGTTTTTAAT GTACTGGGGT TGAACACTCT
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