LOCUS       pSB165_-_pL1R3_pMAS::rLUC-I::tMAS      6054 bp    DNA     circular UNA 3-SEP-2009
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Vector          join(6022..6054,1..4339)
                     /origin="pICH47822 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     misc_feature    complement(23..170)
                     /vntifkey="21"
                     /label="TDNA-LB"
                     /note="shows similarity to T-DNA left border: GenBank
                     Accession Number J01825"
     rep_origin      200..817
                     /standard_name="oriV"
                     /vntifkey="33"
                     /label="oriV"
                     /note="shows similarity to GenBank Accession Number
                     M20134"
     CDS             1015..1872
                     /vntifkey="4"
                     /label="AP r"
     misc_feature    complement(1880..2669)
                     /vntifkey="21"
                     /label="pUC ori"
     rep_origin      complement(2634)
                     /vntifkey="33"
                     /label="ORI"
                     /note="RNaseH cleavage point"
     misc_feature    2680..4161
                     /vntifkey="21"
                     /label="RK2 trfa (no Esp3I)"
     misc_feature    4185..4312
                     /vntifkey="21"
                     /label="RB short"
     misc_feature    4340..4726
                     /feature_type="Insertion"
                     /origin="pICH85281 (2,634bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     387bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     promoter        4344..4726
                     /vntifkey="29"
                     /label="mas prom"
                     /label="mas promoter and 5' UTR (Agrobacterium
                     tumefaciens)"
     misc_feature    4727..5767
                     /feature_type="Insertion"
                     /origin="rLuc codon op + intron_v3 (1,125bp
                     linear)Fragment: from BpiI cut at 40^41 to BpiI cut at
                     1081^1082, 1,041bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    4727..5767
                     /feature_type="Insertion"
                     /origin="pSB123 - pL0_rLuc-I (CDS1) (3,288bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 3272^3273, 1,041bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
                     /label="Insert"
     source          <4727..>5767
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             join(4728..5567,5672..5767)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate
                     Renilla reniformis (sea pansy)"
                     /label="Rluc"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     intron          5568..5671
                     /note="2nd intron from pICH75111"
     misc_feature    5768..6021
                     /feature_type="Insertion"
                     /origin="pICH77901 (2,501bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     254bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     terminator      5770..6021
                     /vntifkey="43"
                     /label="mas term"
ORIGIN
        1 GTGGGGCCCA CTGCATCCAC CCCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG
       61 TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC
      121 CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCAGATCAGG
      181 ATCTCCTTTG CGACGCTCAC CGGGCTGGTT GCCCTCGCCG CTGGGCTGGC GGCCGTCTAT
      241 GGCCCTGCAA ACGCGCCAGA AACGCCGTCG AAGCCGTGTG CGAGACACCG CGGCCGCCGG
      301 CGTTGTGGAT ACCTCGCGGA AAACTTGGCC CTCACTGACA GATGAGGGGC GGACGTTGAC
      361 ACTTGAGGGG CCGACTCACC CGGCGCGGCG TTGACAGATG AGGGGCAGGC TCGATTTCGG
      421 CCGGCGACGT GGAGCTGGCC AGCCTCGCAA ATCGGCGAAA ACGCCTGATT TTACGCGAGT
      481 TTCCCACAGA TGATGTGGAC AAGCCTGGGG ATAAGTGCCC TGCGGTATTG ACACTTGAGG
      541 GGCGCGACTA CTGACAGATG AGGGGCGCGA TCCTTGACAC TTGAGGGGCA GAGTGCTGAC
      601 AGATGAGGGG CGCACCTATT GACATTTGAG GGGCTGTCCA CAGGCAGAAA ATCCAGCATT
      661 TGCAAGGGTT TCCGCCCGTT TTTCGGCCAC CGCTAACCTG TCTTTTAACC TGCTTTTAAA
      721 CCAATATTTA TAAACCTTGT TTTTAACCAG GGCTGCGCCC TGTGCGCGTG ACCGCGCACG
      781 CCGAAGGGGG GTGCCCCCCC TTCTCGAACC CTCCCGGCCC GCTAACGCGG GCCTCCCATC
      841 CCCCCAGGGG CTGCGCCCCT CGGCCGCGAA CGGCCTCACC CCAAAAATGG CAGCGCTGGC
      901 CAATTCGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG
      961 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
     1021 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT
     1081 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG
     1141 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA
     1201 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT
     1261 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
     1321 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT
     1381 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA
     1441 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT
     1501 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA
     1561 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
     1621 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC
     1681 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG TTCTCGCGGT
     1741 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG
     1801 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG
     1861 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA
     1921 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA
     1981 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA
     2041 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG
     2101 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT
     2161 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC
     2221 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG
     2281 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG
     2341 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA
     2401 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC
     2461 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
     2521 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC
     2581 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC
     2641 AGCAACGCGG CCTTTTTACG GTTCCTGGCA GATCCTAGAT GTGGCGCAAC GATGCCGGCG
     2701 ACAAGCAGGA GCGCACCGAC TTCTTCCGCA TCAAGTGTTT TGGCTCTCAG GCCGAGGCCC
     2761 ACGGCAAGTA TTTGGGCAAG GGGTCGCTGG TATTCGTGCA GGGCAAGATT CGGAATACCA
     2821 AGTACGAGAA GGACGGCCAG ACGGTCTACG GGACCGACTT CATTGCCGAT AAGGTGGATT
     2881 ATCTGGACAC CAAGGCACCA GGCGGGTCAA ATCAGGAATA AGGGCACATT GCCCCGGCGT
     2941 GAGTCGGGGC AATCCCGCAA GGAGGGTGAA TGAATCGGAC GTTTGACCGG AAGGCATACA
     3001 GGCAAGAACT GATCGACGCG GGGTTTTCCG CCGAGGATGC CGAAACCATC GCAAGCCGCA
     3061 CCGTCATGCG TGCGCCCCGC GAAACCTTCC AGTCCGTCGG CTCGATGGTC CAGCAAGCTA
     3121 CGGCCAAGAT CGAGCGCGAC AGCGTGCAAC TGGCTCCCCC TGCCCTGCCC GCGCCATCGG
     3181 CCGCCGTGGA GCGTTCGCGT CGTCTTGAAC AGGAGGCGGC AGGTTTGGCG AAGTCGATGA
     3241 CCATCGACAC GCGAGGAACT ATGACGACCA AGAAGCGAAA AACCGCCGGC GAGGACCTGG
     3301 CAAAACAGGT CAGCGAGGCC AAGCAGGCCG CGTTGCTGAA ACACACGAAG CAGCAGATCA
     3361 AGGAAATGCA GCTTTCCTTG TTCGATATTG CGCCGTGGCC GGACACGATG CGAGCGATGC
     3421 CAAACGACAC GGCCCGCTCT GCCCTGTTCA CCACGCGCAA CAAGAAAATC CCGCGCGAGG
     3481 CGCTGCAAAA CAAGGTCATT TTCCACGTCA ACAAGGACGT GAAGATCACC TACACCGGCG
     3541 TCGAGCTGCG GGCCGACGAT GACGAACTGG TGTGGCAGCA GGTGTTGGAG TACGCGAAGC
     3601 GCACCCCTAT CGGCGAGCCG ATCACCTTCA CGTTCTACGA GCTTTGCCAG GACCTGGGCT
     3661 GGTCGATCAA TGGCCGGTAT TACACGAAGG CCGAGGAATG CCTGTCGCGC CTACAGGCGA
     3721 CGGCGATGGG CTTCACGTCC GACCGCGTTG GGCACCTGGA ATCGGTGTCG CTGCTGCACC
     3781 GCTTCCGCGT CCTGGACCGT GGCAAGAAAA CGTCCCGTTG CCAGGTCCTG ATCGACGAGG
     3841 AAATCGTCGT GCTGTTTGCT GGCGACCACT ACACGAAATT CATATGGGAG AAGTACCGCA
     3901 AGCTGTCGCC GACGGCCCGA CGGATGTTCG ACTATTTCAG CTCGCACCGG GAGCCGTACC
     3961 CGCTCAAGCT GGAAACCTTC CGCCTCATGT GCGGATCGGA TTCCACCCGC GTGAAGAAGT
     4021 GGCGCGAGCA GGTCGGCGAA GCCTGCGAAG AGTTGCGAGG CAGCGGCCTG GTGGAACACG
     4081 CCTGGGTCAA TGATGACCTG GTGCATTGCA AACGCTAGGG CCTTGTGGGG TCAGTTCCGG
     4141 CTGGGGGTTC AGCAGCCAGC GCCTGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA
     4201 ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC
     4261 TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACCACT
     4321 TCGTGCAGAA GACAAGTAAG GAGATTTTTC AAATCAGTGC GCAAGACGTG ACGTAAGTAT
     4381 CCGAGTCAGT TTTTATTTTT CTACTAATTT GGTCGTTTAT TTCGGCGTGT AGGACATGGC
     4441 AACCGGGCCT GAATTTCGCG GGTATTCTGT TTCTATTCCA ACTTTTTCTT GATCCGCAGC
     4501 CATTAACGAC TTTTGAATAG ATACGCTGAC ACGCCAAGCC TCGCTAGTCA AAAGTGTACC
     4561 AAACAACGCT TTACAGCAAG AACGGAATGC GCGTGACGCT CGCGGTGACG CCATTTCGCC
     4621 TTTTCAGAAA TGGATAAATA GCCTTGCTTC CTATTATATC TTCCCAAATT ACCAATACAT
     4681 TACACTAGCA TCTGAATTTC ATAACCAATC TCGATACACC AAATCGAATG ACTTCTAAGG
     4741 TTTATGATCC TGAACAAAGA AAGAGAATGA TTACTGGACC TCAATGGTGG GCTAGATGTA
     4801 AGCAAATGAA TGTTCTTGAT TCTTTTATTA ATTATTATGA TTCTGAAAAG CATGCTGAAA
     4861 ATGCTGTTAT TTTTCTTCAT GGAAATGCTG CTTCTTCTTA TCTTTGGAGA CATGTTGTTC
     4921 CACATATTGA ACCAGTTGCT AGATGTATTA TTCCAGATCT TATTGGTATG GGAAAGTCTG
     4981 GTAAGTCTGG TAATGGTTCT TATAGACTTC TTGATCATTA TAAGTATCTT ACTGCTTGGT
     5041 TTGAACTTCT TAATCTTCCA AAGAAGATTA TTTTTGTTGG ACATGATTGG GGTGCTTGTT
     5101 TGGCTTTTCA TTATTCTTAT GAACATCAAG ATAAGATTAA GGCTATTGTT CATGCTGAAT
     5161 CTGTTGTTGA TGTTATTGAA TCTTGGGATG AATGGCCTGA TATTGAAGAA GATATTGCTT
     5221 TGATTAAGTC TGAAGAAGGA GAAAAGATGG TTTTGGAAAA TAATTTCTTT GTTGAAACTA
     5281 TGTTGCCATC TAAGATTATG AGAAAGCTTG AACCAGAAGA ATTTGCTGCT TATCTTGAAC
     5341 CATTTAAGGA AAAGGGTGAA GTTAGAAGGC CAACTCTTTC TTGGCCTAGA GAAATTCCTC
     5401 TTGTTAAGGG TGGTAAGCCT GATGTTGTTC AAATTGTTAG AAATTATAAT GCTTATCTTA
     5461 GAGCTTCTGA TGATCTTCCA AAGATGTTTA TTGAATCTGA TCCAGGATTC TTTTCTAATG
     5521 CTATTGTTGA AGGTGCTAAG AAGTTTCCTA ATACTGAATT TGTTAAGGTA CTTCATGCTT
     5581 CAACGTGTAA CTTAAGAGAT ACTGTGTGAA ATTTTATATT TCCATACATT TGCTTGACCT
     5641 TTGCTTTTTG TCAATTTTTT TCCCCTTACA GGTTAAGGGA CTTCATTTTT CTCAAGAAGA
     5701 TGCTCCTGAT GAAATGGGAA AGTATATTAA GTCTTTTGTT GAAAGAGTTC TTAAGAATGA
     5761 ACAATGAGCT TGGACTCCCA TGTTGGCAAA GGCAACCAAA CAAACAATGA ATGATCCGCT
     5821 CCTGCATATG GGGCGGTTTG AGTATTTCAA CTGCCATTTG GGCTGAATTG TAGACATGCT
     5881 CCTGTCAGAA ATTCCGTGAT CTTACTCAAT ATTCAGTAAT CTCGGCCAAT ATCCTAAATG
     5941 TGCGTGGCTT TATCTGTCTT TGTATTGTTT CATCAATTCA TGTAACGTTT GCTTTTCTTA
     6001 TGAATTTTCA AATAAATTAT CCGCTGAGCT CGAATTCTAG TTTGTCTTCA CAGA
//
