LOCUS       pSB167_-_pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS     10549 bp    DNA     circular SYN 21-NOV-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAGM4723 cor
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2013 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     pSB90 was confrimed by digest with EcoRV & BpiI and by sequencing (8/29/17). 
            
FEATURES             Location/Qualifiers
     Vector          join(4720..10549,1)
                     /origin="pSB90 - pAGM4723_VirGN54D (NEBuilder) (14,072bp
                     circular)Target vector: from BpiI cut at 8096^8097 to
                     BpiI cut at 1^2, 5,977bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     misc_feature    2..2974
                     /feature_type="Insertion"
                     /origin="pSB85 - pL1F1_2x35::fLUC-I::Tnos (7,325bp
                     circular)Fragment: from BpiI cut at 4335^4336 to BpiI cut
                     at 7308^7309, 2,973bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(2975..>2978,<6..21))
                     /origin="pICH47732 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        22..774
                     /vntifkey="29"
                     /label="2x35S pro"
                     /label="2x CaMV 35S promoter"
     misc_feature    complement(22..864)
                     /feature_type="Insertion"
                     /origin="pICH51288 (3,090bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     843bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     5'UTR           791..852
                     /vntifkey="52"
                     /label="Omega leader"
                     /label="TMV Omega"
     misc_feature    861..1005
                     /feature_type="Insertion"
                     /origin="fLuc part1 (169bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 155^156, 145bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     CDS             862..1005
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLUC-I"
     misc_feature    complement(865..>1009)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(865..2707)
                     /feature_type="Insertion"
                     /origin="pSB63 - pL0_fLuc-I (CDS1) (4,090bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 4074^4075, 1,843bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     intron          1006..1194
                     /note="U5 small nuclear ribonucleoprotein component
                     (Arabidopsis thaliana)"
     misc_feature    1006..1190
                     /feature_type="Insertion"
                     /origin="fLuc part2 (209bp linear)Fragment: from BpiI cut
                     at 10^11 to BpiI cut at 195^196, 185bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    complement(<1010..>1194)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    1191..2703
                     /feature_type="Insertion"
                     /origin="fLuc part3 (1,537bp linear)Fragment: from BpiI
                     cut at 10^11 to BpiI cut at 1523^1524,
                     1,513bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             1195..2703
                     /vntifkey="4"
                     /label="coding sequence of Luciferase gene from
                     Photinuspyralis"
                     /label="Luciferase (Photinus pyralis)"
                     /note="from MoClo"
                     /label="fLUC-I"
     misc_feature    complement(<1195..>2707)
                     /feature_type="Insertion"
                     /origin="pICSL80001 (3,901bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     1,654bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(2708..2974)
                     /feature_type="Insertion"
                     /origin="pICH41421 (2,514bp circular)Fragment: from
                     Eco31I cut at 2231^2232 to Eco31I cut at 2498^2499,
                     267bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      2708..2970
                     /vntifkey="43"
                     /label="nost"
     misc_feature    2975..2993
                     /feature_type="Insertion"
                     /origin="pICH54022 (4,368bp circular)Fragment: from BpiI
                     cut at 4349^4350 to BpiI cut at -1^1, 19bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     misc_feature    2994..4695
                     /feature_type="Insertion"
                     /origin="pSB165 - pL1R3_Pmas::rLuc-I::Tmas (6,054bp
                     circular)Fragment: from BpiI cut at 4335^4336 to BpiI cut
                     at 6037^6038, 1,702bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label="Insert"
     Vector          complement(join(4696..>4699,<2998..3013))
                     /origin="pICH47822 (4,968bp circular)Target vector: from
                     Eco31I cut at 4935^4936 to Eco31I cut at 4339^4340,
                     4,372bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     misc_feature    complement(3014..3267)
                     /feature_type="Insertion"
                     /origin="pICH77901 (2,501bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     254bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     terminator      complement(3014..3265)
                     /vntifkey="43"
                     /label="mas term"
     CDS             complement(join(3268..3363,3468..4307))
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate
                     Renilla reniformis (sea pansy)"
                     /label="Rluc"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
                     /label="rLUC-I"
     misc_feature    complement(3268..4308)
                     /feature_type="Insertion"
                     /origin="rLuc codon op + intron_v3 (1,125bp
                     linear)Fragment: from BpiI cut at 40^41 to BpiI cut at
                     1081^1082, 1,041bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     misc_feature    complement(3268..4308)
                     /feature_type="Insertion"
                     /origin="pSB123 - pL0_rLuc-I (CDS1) (3,288bp
                     circular)Fragment: from Eco31I cut at 2231^2232 to Eco31I
                     cut at 3272^3273, 1,041bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
                     /label="Insert"
     source          complement(<3268..>4308)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          complement(3364..3467)
                     /note="2nd intron from pICH75111"
     promoter        complement(4309..4691)
                     /vntifkey="29"
                     /label="mas prom"
                     /label="mas promoter and 5' UTR (Agrobacterium
                     tumefaciens)"
     misc_feature    complement(4309..4695)
                     /feature_type="Insertion"
                     /origin="pICH85281 (2,634bp circular)Fragment: from
                     Eco31I cut at 2247^2248 to Eco31I cut at -1^1,
                     387bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    4696..4719
                     /feature_type="Insertion"
                     /origin="pICH41766 (3,318bp circular)Fragment: from BpiI
                     cut at 2847^2848 to BpiI cut at 2871^2872,
                     24bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label="Insert"
     misc_feature    4700..4719
                     /vntifkey="21"
                     /label="EL3"
     misc_feature    complement(4737..4864)
                     /label="RB"
                     /note="RB"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    4869..7503
                     /label="pVS1 fragment short"
                     /note="pVS1 fragment short"
                     /note="color: #66cc99; direction: RIGHT"
     CDS             5059..5688
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 StaA"
     CDS             6122..7189
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 RepA"
     rep_origin      7255..7449
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     promoter        7511..7615
                     /label="AmpR promoter"
     CDS             7616..8407
                     /codon_start=1
                     /label="NPTII"
                     /note="NPTII"
                     /note="color: #ff9966"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       8098
                     /note="A to G (BpiI removed)"
                     /note="color: #3366cc"
     variation       8297
                     /note="A to C, I to L"
                     /note="color: #3366cc"
     misc_feature    8540..9692
                     /label="VirGN54D insert"
     CDS             8670..9473
                     /label="VirGN54D"
     misc_feature    9723..10337
                     /label="rep - pMB1"
                     /note="rep - pMB1"
                     /note="color: #66cc99; direction: RIGHT"
     variation       9881
                     /note="C to T"
                     /note="color: #3366cc"
     rep_origin      10322
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     misc_feature    complement(10389..10539)
                     /label="LB"
                     /note="LB"
                     /note="color: #66cc99; direction: LEFT"
ORIGIN
        1 GTGCCGAATT CGGATCCGGA GGTCAACATG GTGGAGCACG ACACTCTGGT CTACTCCAAA
       61 AATGTCAAAG ATACAGTCTC AGAAGATCAA AGGGCTATTG AGACTTTTCA ACAAAGGATA
      121 ATTTCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CGAAAGGACA
      181 GTAGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCATT
      241 CAAGATCTCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG
      301 GAAAAAGAAG AGGTTCCAAC CACGTCTACA AAGCAAGTGG ATTGATGTGA TAACATGGTG
      361 GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA CAGTCTCAGA AGATCAAAGG
      421 GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC TCCTCGGATT CCATTGCCCA
      481 GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG GTGGCTCCTA CAAATGCCAT
      541 CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG CCGACAGTGG TCCCAAAGAT
      601 GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGAGG TTCCAACCAC GTCTACAAAG
      661 CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT
      721 TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTCGAGT
      781 ATAAGAGCTC ATTTTTACAA CAATTACCAA CAACAACAAA CAACAAACAA CATTACAATT
      841 ACATTTACAA TTATCGATAC AATGGAGGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA
      901 TTCTATCCTC TAGAGGATGG AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC
      961 GCCCTGGTTC CTGGAACAAT TGCTTTTACA GATGCACATA TCGAGGTAAG TTTCTGCTTC
     1021 TACCTTTGAT ATATATATAA TAATTATCAT TAATTAGTAG TAATATAATA TTTCAAATAT
     1081 TTTTTTCAAA ATAAAAGAAT GTAGTATATA GCAATTGCTT TTCTGTAGTT TATAAGTGTG
     1141 TATATTTTAA TTTATAACTT TTCTAATATA TGACCAAAAT TTGTTGATGT GCAGGTGAAC
     1201 ATCACGTACG CGGAATACTT CGAAATGTCC GTTCGGTTGG CAGAAGCTAT GAAACGATAT
     1261 GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG
     1321 CCGGTGTTGG GCGCGTTATT TATCGGAGTT GCAGTTGCGC CCGCGAACGA CATTTATAAT
     1381 GAACGTGAAT TGCTCAACAG TATGAACATT TCGCAGCCTA CCGTAGTGTT TGTTTCCAAA
     1441 AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAATTAC CAATAATCCA GAAAATTATT
     1501 ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA TGTACACGTT CGTCACATCT
     1561 CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTACCAG AGTCCTTTGA TCGTGACAAA
     1621 ACAATTGCAC TGATAATGAA TTCCTCTGGA TCTACTGGGT TACCTAAGGG TGTGGCCCTT
     1681 CCGCATAGAA CTGCCTGCGT CAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA
     1741 ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT TGGAATGTTT
     1801 ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT TAATGTATAG ATTTGAAGAA
     1861 GAGCTGTTTT TACGATCCCT TCAGGATTAC AAAATTCAAA GTGCGTTGCT AGTACCAACC
     1921 CTATTTTCAT TCTTCGCCAA AAGCACTCTG ATTGACAAAT ACGATTTATC TAATTTACAC
     1981 GAAATTGCTT CTGGGGGCGC ACCTCTTTCG AAAGAAGTCG GGGAAGCGGT TGCAAAACGC
     2041 TTCCATCTTC CAGGGATACG ACAAGGATAT GGGCTCACTG AGACTACATC AGCTATTCTG
     2101 ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA AAGTTGTTCC ATTTTTTGAA
     2161 GCGAAGGTTG TGGATCTGGA TACCGGGAAA ACGCTGGGCG TTAATCAGAG AGGCGAATTA
     2221 TGTGTCAGAG GACCTATGAT TATGTCCGGT TATGTAAACA ATCCGGAAGC GACCAACGCC
     2281 TTGATTGACA AGGATGGATG GCTACATTCT GGAGACATAG CTTACTGGGA CGAGGACGAA
     2341 CACTTCTTCA TAGTTGACCG CTTGAAGTCT TTAATTAAAT ACAAAGGATA TCAGGTGGCC
     2401 CCCGCTGAAT TGGAATCGAT ATTGTTACAA CACCCCAACA TCTTCGACGC GGGCGTGGCA
     2461 GGTCTGCCCG ACGATGACGC CGGTGAACTT CCCGCCGCCG TTGTTGTTTT GGAGCACGGA
     2521 AAGACGATGA CGGAAAAAGA GATCGTGGAT TACGTCGCCA GTCAAGTAAC AACCGCGAAA
     2581 AAGTTGCGCG GAGGAGTTGT GTTTGTGGAC GAAGTACCGA AAGGTCTTAC CGGAAAACTC
     2641 GACGCAAGAA AAATCAGAGA GATCCTCATA AAGGCCAAGA AGGGCGGAAA GTCCAAATTG
     2701 TAAGCTTGTC AAGCAGATCG TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT
     2761 GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA
     2821 ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA
     2881 TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG
     2941 CGCGCGGTGT CATCTATGTT ACTAGATCGA CGCTGCAAGG TTTTGACGGG TTTACTAGAA
     3001 TTCGAGCTCA GCGGATAATT TATTTGAAAA TTCATAAGAA AAGCAAACGT TACATGAATT
     3061 GATGAAACAA TACAAAGACA GATAAAGCCA CGCACATTTA GGATATTGGC CGAGATTACT
     3121 GAATATTGAG TAAGATCACG GAATTTCTGA CAGGAGCATG TCTACAATTC AGCCCAAATG
     3181 GCAGTTGAAA TACTCAAACC GCCCCATATG CAGGAGCGGA TCATTCATTG TTTGTTTGGT
     3241 TGCCTTTGCC AACATGGGAG TCCAAGCTCA TTGTTCATTC TTAAGAACTC TTTCAACAAA
     3301 AGACTTAATA TACTTTCCCA TTTCATCAGG AGCATCTTCT TGAGAAAAAT GAAGTCCCTT
     3361 AACCTGTAAG GGGAAAAAAA TTGACAAAAA GCAAAGGTCA AGCAAATGTA TGGAAATATA
     3421 AAATTTCACA CAGTATCTCT TAAGTTACAC GTTGAAGCAT GAAGTACCTT AACAAATTCA
     3481 GTATTAGGAA ACTTCTTAGC ACCTTCAACA ATAGCATTAG AAAAGAATCC TGGATCAGAT
     3541 TCAATAAACA TCTTTGGAAG ATCATCAGAA GCTCTAAGAT AAGCATTATA ATTTCTAACA
     3601 ATTTGAACAA CATCAGGCTT ACCACCCTTA ACAAGAGGAA TTTCTCTAGG CCAAGAAAGA
     3661 GTTGGCCTTC TAACTTCACC CTTTTCCTTA AATGGTTCAA GATAAGCAGC AAATTCTTCT
     3721 GGTTCAAGCT TTCTCATAAT CTTAGATGGC AACATAGTTT CAACAAAGAA ATTATTTTCC
     3781 AAAACCATCT TTTCTCCTTC TTCAGACTTA ATCAAAGCAA TATCTTCTTC AATATCAGGC
     3841 CATTCATCCC AAGATTCAAT AACATCAACA ACAGATTCAG CATGAACAAT AGCCTTAATC
     3901 TTATCTTGAT GTTCATAAGA ATAATGAAAA GCCAAACAAG CACCCCAATC ATGTCCAACA
     3961 AAAATAATCT TCTTTGGAAG ATTAAGAAGT TCAAACCAAG CAGTAAGATA CTTATAATGA
     4021 TCAAGAAGTC TATAAGAACC ATTACCAGAC TTACCAGACT TTCCCATACC AATAAGATCT
     4081 GGAATAATAC ATCTAGCAAC TGGTTCAATA TGTGGAACAA CATGTCTCCA AAGATAAGAA
     4141 GAAGCAGCAT TTCCATGAAG AAAAATAACA GCATTTTCAG CATGCTTTTC AGAATCATAA
     4201 TAATTAATAA AAGAATCAAG AACATTCATT TGCTTACATC TAGCCCACCA TTGAGGTCCA
     4261 GTAATCATTC TCTTTCTTTG TTCAGGATCA TAAACCTTAG AAGTCATTCG ATTTGGTGTA
     4321 TCGAGATTGG TTATGAAATT CAGATGCTAG TGTAATGTAT TGGTAATTTG GGAAGATATA
     4381 ATAGGAAGCA AGGCTATTTA TCCATTTCTG AAAAGGCGAA ATGGCGTCAC CGCGAGCGTC
     4441 ACGCGCATTC CGTTCTTGCT GTAAAGCGTT GTTTGGTACA CTTTTGACTA GCGAGGCTTG
     4501 GCGTGTCAGC GTATCTATTC AAAAGTCGTT AATGGCTGCG GATCAAGAAA AAGTTGGAAT
     4561 AGAAACAGAA TACCCGCGAA ATTCAGGCCC GGTTGCCATG TCCTACACGC CGAAATAAAC
     4621 GACCAAATTA GTAGAAAAAT AAAAACTGAC TCGGATACTT ACGTCACGTC TTGCGCACTG
     4681 ATTTGAAAAA TCTCCTTACG AGGATGCACA TGTGACCGAG GGACACGAAG TGATCCGTTT
     4741 AAACTATCAG TGTTTGACAG GATATATTGG CGGGTAAACC TAAGAGAAAA GAGCGTTTAT
     4801 TAGAATAATC GGATATTTAA AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT
     4861 GCCAGCCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG
     4921 GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT
     4981 TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA
     5041 AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC
     5101 AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG
     5161 TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA
     5221 CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC
     5281 CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG
     5341 ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC
     5401 ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA
     5461 GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG
     5521 GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC
     5581 CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC
     5641 CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA
     5701 AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA
     5761 GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC
     5821 AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA
     5881 TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA
     5941 ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC
     6001 ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC
     6061 TGGGTTGTCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA
     6121 CGGTCGCAAA CCATCCGGCC CGGTACAAAT CGGCGCGGCG CTGGGTGATG ACCTGGTGGA
     6181 GAAGTTGAAG GCCGCGCAGG CCGCCCAGCG GCAACGCATC GAGGCAGAAG CACGCCCCGG
     6241 TGAATCGTGG CAAGCGGCCG CTGATCGAAT CCGCAAAGAA TCCCGGCAAC CGCCGGCAGC
     6301 CGGTGCGCCG TCGATTAGGA AGCCGCCCAA GGGCGACGAG CAACCAGATT TTTTCGTTCC
     6361 GATGCTCTAT GACGTGGGCA CCCGCGATAG TCGCAGCATC ATGGACGTGG CCGTTTTCCG
     6421 TCTGTCGAAG CGTGACCGAC GAGCTGGCGA GGTGATCCGC TACGAGCTTC CAGACGGGCA
     6481 CGTAGAGGTT TCCGCAGGGC CGGCCGGCAT GGCCAGTGTG TGGGATTACG ACCTGGTACT
     6541 GATGGCGGTT TCCCATCTAA CCGAATCCAT GAACCGATAC CGGGAAGGGA AGGGAGACAA
     6601 GCCCGGCCGC GTGTTCCGTC CACACGTTGC GGACGTACTC AAGTTCTGCC GGCGAGCCGA
     6661 TGGCGGAAAG CAGAAAGACG ACCTGGTAGA AACCTGCATT CGGTTAAACA CCACGCACGT
     6721 TGCCATGCAG CGTACGAAGA AGGCCAAGAA CGGCCGCCTG GTGACGGTAT CCGAGGGTGA
     6781 AGCCTTGATT AGCCGCTACA AGATCGTAAA GAGCGAAACC GGGCGGCCGG AGTACATCGA
     6841 GATCGAGCTA GCTGATTGGA TGTACCGCGA GATCACAGAA GGCAAGAACC CGGACGTGCT
     6901 GACGGTTCAC CCCGATTACT TTTTGATCGA TCCCGGCATC GGCCGTTTTC TCTACCGCCT
     6961 GGCACGCCGC GCCGCAGGCA AGGCAGAAGC CAGATGGTTG TTCAAGACGA TCTACGAACG
     7021 CAGTGGCAGC GCCGGAGAGT TCAAGAAGTT CTGTTTCACC GTGCGCAAGC TGATCGGGTC
     7081 AAATGACCTG CCGGAGTACG ATTTGAAGGA GGAGGCGGGG CAGGCTGGCC CGATCCTAGT
     7141 CATGCGCTAC CGCAACCTGA TCGAGGGCGA AGCATCCGCC GGTTCCTAAT GTACGGAGCA
     7201 GATGCTAGGG CAAATTGCCC TAGCAGGGGA AAAAGGTCGA AAAAGCTTCT TTCCTGTGGA
     7261 TAGCACGTAC ATTGGGAACC CAAAGCCGTA CATTGGGAAC CGGAACCCGT ACATTGGGAA
     7321 CCCAAAGCCG TACATTGGGA ACCGGTCACA CATGTAAGTG ACTGATATAA AAGAGAAAAA
     7381 AGGCGATTTT TCCGCCTAAA ACTCTTTAAA ACTTATTAAA ACTCTTAAAA CCCGCCTGGC
     7441 CTGTGCATAA CTGTCTGGCC AGCGCACAGC CGAACAGCTG CAAAAAGCGC CCACATCAAG
     7501 GCTCCGAGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
     7561 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGGC
     7621 TAAAATGAGA ATATCACCGG AATTGAAAAA ACTGATCGAA AAATACCGCT GCGTAAAAGA
     7681 TACGGAAGGA ATGTCTCCTG CTAAGGTATA TAAGCTGGTG GGAGAAAATG AAAACCTATA
     7741 TTTAAAAATG ACGGACAGCC GGTATAAAGG GACCACCTAT GATGTGGAAC GGGAAAAGGA
     7801 CATGATGCTA TGGCTGGAAG GAAAGCTGCC TGTTCCAAAG GTCCTGCACT TTGAACGGCA
     7861 TGATGGCTGG AGCAATCTGC TCATGAGTGA GGCCGATGGC GTCCTTTGCT CGGAAGAGTA
     7921 TGAAGATGAA CAAAGCCCTG AAAAGATTAT CGAGCTGTAT GCGGAGTGCA TCAGGCTCTT
     7981 TCACTCCATC GACATATCGG ATTGTCCCTA TACGAATAGC TTAGACAGCC GCTTAGCCGA
     8041 ATTGGATTAC TTACTGAATA ACGATCTGGC CGATGTGGAT TGCGAAAACT GGGAAGAGGA
     8101 CACTCCATTT AAAGATCCGC GCGAGCTGTA TGATTTTTTA AAGACGGAAA AGCCCGAAGA
     8161 GGAACTTGTC TTTTCCCACG GCGACCTGGG AGACAGCAAC ATCTTTGTGA AAGATGGCAA
     8221 AGTAAGTGGC TTTATTGATC TTGGGAGAAG CGGCAGGGCG GACAAGTGGT ATGACATTGC
     8281 CTTCTGCGTC CGGTCGCTCA GGGAGGATAT CGGGGAAGAA CAGTATGTCG AGCTATTTTT
     8341 TGACTTACTG GGGATCAAGC CTGATTGGGA GAAAATAAAA TATTATATTT TACTGGATGA
     8401 ATTGTTTTAG CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     8461 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     8521 TTAACGTGAG TTTTCGTTCT AGCTGTAACC TCGAAGCGTT TCACTTGTAA CAACGATTGA
     8581 GAATTTTGTC ATAAAATTGA AATACTTGGT TCGCATTTTC GTCATCCGCG GTCAGCCGCA
     8641 ATTCTGACGA ACTGCCCATT TAGCTGGAGA TGATTGTACA TCCTTCACGT GAAAATTTCT
     8701 CAAGCGCTGT GAACAAGGGT TCAGATTTTA GATTGAAAGG TGAGCCGTTG AAACACGTTC
     8761 TTCTTGTCGA TGACGACGTC GCTATGCGGC ATCTTATTAT TGAATACCTT ACGATCCACG
     8821 CCTTCAAAGT GACCGCGGTA GCCGACAGCA CCCAGTTCAC AAGAGTACTC TCTTCCGCGA
     8881 CGGTCGATGT CGTGGTTGTT GATCTAGATT TAGGTCGTGA AGATGGGCTC GAGATCGTTC
     8941 GTAATCTGGC GGCAAAGTCT GATATTCCAA TCATAATTAT CAGTGGCGAC CGCCTTGAGG
     9001 AGACGGATAA AGTTGTTGCA CTCGAGCTAG GAGCAAGTGA TTTTATCGCT AAGCCGTTCA
     9061 GTATCAGAGA GTTTCTAGCA CGCATTCGGG TTGCCTTGCG CGTGCGCCCC AACGTTGTCC
     9121 GCTCCAAAGA CCGACGGTCT TTTTGTTTTA CTGACTGGAC ACTTAATCTC AGGCAACGTC
     9181 GCTTGATGTC CGAAGCTGGC GGTGAGGTGA AACTTACGGC AGGTGAGTTC AATCTTCTCC
     9241 TCGCGTTTTT AGAGAAACCC CGCGACGTTC TATCGCGCGA GCAACTTCTC ATTGCCAGTC
     9301 GAGTACGCGA CGAGGAGGTT TATGACAGGA GTATAGATGT TCTCATTTTG AGGCTGCGCC
     9361 GCAAACTTGA GGCGGATCCG TCAAGCCCTC AACTGATAAA AACAGCAAGA GGTGCCGGTT
     9421 ATTTCTTTGA CGCGGACGTG CAGGTTTCGC ACGGGGGGAC GATGGCAGCC TGAGCCAATT
     9481 GCATTTGCCT CTTAATTATC TGGCTCAAAG GGTGACTGAG GAGTAAGCGA TGTGCCCATC
     9541 ACACTGACCA CCAAGACGGG GGTTCGTTTT GAAGATCACC CTTCAGACCC AAGGCCAGTG
     9601 TGCGTTGATC GCGGCCACAC TGCAAATTGA CATACTGTCG ATAAAGGGGC GTTGGACGAG
     9661 AACCCATTCG TCAGGATACT GGCTCGTGAT CTCACTGAGC GTCAGACCCC GTAGAAAAGA
     9721 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     9781 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     9841 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     9901 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     9961 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
    10021 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
    10081 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
    10141 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
    10201 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
    10261 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
    10321 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGCTCGG ATCTGTTGGA CCGGACAGTA
    10381 GTCATGGTTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG
    10441 AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA
    10501 CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGGGGTT GAACACTCT
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