LOCUS       pDONR223_GABARAP_no_stop_codon    3144 bp      DNA     circular   11/02/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) BP reaction between PCR(AttB-GABARAP cds) as an 
            Expression fragment and pDONR223 as a Donor vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T2_terminator"complement(268..295) \ 
     terminator      complement(429..472)
                     /label=rrnB T1 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T1_terminator" - complement(427..470) \ 
     misc_feature    524..546
                     /label=M13 pUC fwd primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_pUC_fwd_primer" 522..544 \ 
     promoter        539..555
                     /label=M13 forward20 primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_forward20_primer" 537..553 \ 
     misc_feature    complement(571..654)
                     /label=attL1
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL1"complement(569..652) \ 
     misc_feature    1037..1124
                     /label=attL2
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL2" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCD \ 
     promoter        complement(1142..1160)
                     /label=T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="T7_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ 
     promoter        complement(1161..1179)
                     /label=M13 reverse primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ 
     gene            1393..2403
                     /label=spect
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="spect" - gene="spect" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCD \ 
     rep_origin      2480..3099
                     /label=pBR322_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBR322_origin" - translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA - RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL - AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE - VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLA \ 
                     658..1023
                     /label=Insert from PCR(AttB-GABARAP cds) [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from PCR(AttB-GABARAP cds) into pDONR223 [BP] reaction \ 
                     1024..1037
                     /label=Insert from PCR(AttB-GABARAP cds) [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from PCR(AttB-GABARAP cds) into pDONR223 [BP] reaction \ 
ORIGIN
        1 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 
       61 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 
      121 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 
      181 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TACGCGTACC 
      241 GCTAGCCAGG AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT 
      301 TAGTTTGATG CCTGGCAGTT TATGGCGGGC GTCCTGCCCG CCACCCTCCG GGCCGTTGCT 
      361 TCACAACGTT CAAATCCGCT CCCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA 
      421 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC 
      481 TGGCAGTTCC CTACTCTCGC GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT 
      541 AAAACGACGG CCAGTCTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG 
      601 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA 
      661 AAAGCAGGCT TAATGAAGTT CGTGTACAAA GAAGAGCATC CGTTCGAGAA GCGCCGCTCT 
      721 GAGGGCGAGA AGATCCGAAA GAAATACCCG GACCGGGTGC CGGTGATAGT AGAAAAGGCT 
      781 CCCAAAGCTC GGATAGGAGA CCTGGACAAA AAGAAATACC TGGTGCCTTC TGATCTCACA 
      841 GTTGGTCAGT TCTACTTCTT GATCCGGAAG CGAATTCATC TCCGAGCTGA GGATGCCTTG 
      901 TTTTTCTTTG TCAACAATGT CATTCCACCC ACCAGTGCCA CAATGGGTCA GCTGTACCAG 
      961 GAACACCATG AAGAAGACTT CTTTCTCTAC ATTGCCTACA GTGACGAAAG TGTCTACGGT 
     1021 CTGTACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT 
     1081 TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC 
     1141 CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT 
     1201 CAAAATCTCT GATGTTACAT TGCACAAGAT AAAAATATAT CATCATGCCT CCTCTAGACC 
     1261 AGCCAGGACA GAAATGCCTC GACTTCGCTG CTGCCCAAGG TTGCCGGGTG ACGCACACCG 
     1321 TGGAAACGGA TGAAGGCACG AACCCAGTGG ACATAAGCCT GTTCGGTTCG TAAGCTGTAA 
     1381 TGCAAGTAGC GTATGCGCTC ACGCAACTGG TCCAGAACCT TGACCGAACG CAGCGGTGGT 
     1441 AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGGGGT ACAGTCTATG 
     1501 CCTCGGGCAT CCAAGCAGCA AGCGCGTTAC GCCGTGGGTC GATGTTTGAT GTTATGGAGC 
     1561 AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAAACAT CATGAGGGAA 
     1621 GCGGTGATCG CCGAAGTATC GACTCAACTA TCAGAGGTAG TTGGCGTCAT CGAGCGCCAT 
     1681 CTCGAACCGA CGTTGCTGGC CGTACATTTG TACGGCTCCG CAGTGGATGG CGGCCTGAAG 
     1741 CCACACAGTG ATATTGATTT GCTGGTTACG GTGACCGTAA GGCTTGATGA AACAACGCGG 
     1801 CGAGCTTTGA TCAACGACCT TTTGGAAACT TCGGCTTCCC CTGGAGAGAG CGAGATTCTC 
     1861 CGCGCTGTAG AAGTCACCAT TGTTGTGCAC GACGACATCA TTCCGTGGCG TTATCCAGCT 
     1921 AAGCGCGAAC TGCAATTTGG AGAATGGCAG CGCAATGACA TTCTTGCAGG TATCTTCGAG 
     1981 CCAGCCACGA TCGACATTGA TCTGGCTATC TTGCTGACAA AAGCAAGAGA ACATAGCGTT 
     2041 GCCTTGGTAG GTCCAGCGGC GGAGGAACTC TTTGATCCGG TTCCTGAACA GGATCTATTT 
     2101 GAGGCGCTAA ATGAAACCTT AACGCTATGG AACTCGCCGC CCGACTGGGC TGGCGATGAG 
     2161 CGAAATGTAG TGCTTACGTT GTCCCGCATT TGGTACAGCG CAGTAACCGG CAAAATCGCG 
     2221 CCGAAGGATG TCGCTGCCGA CTGGGCAATG GAGCGCCTGC CGGCCCAGTA TCAGCCCGTC 
     2281 ATACTTGAAG CTAGACAGGC TTATCTTGGA CAAGAAGAAG ATCGCTTGGC CTCGCGCGCA 
     2341 GATCAGTTGG AAGAATTTGT CCACTACGTG AAAGGCGAGA TCACCAAGGT AGTCGGCAAA 
     2401 TAACCCTCGA GCCACCCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA 
     2461 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 
     2521 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 
     2581 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 
     2641 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 
     2701 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 
     2761 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 
     2821 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 
     2881 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 
     2941 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 
     3001 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 
     3061 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 
     3121 TTTTGCTGGC CTTTTGCTCA CATG
//