LOCUS       pLenti_PGK_Puro_GFP-GABARAPL1_G116    8952 bp      DNA     circular   11/02/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pLenti PGK GFP Puro addgene-19070 [7820  nt] :
             (SalI[5444] / BamHI[4696]) to PCR(lentipuro-EGFP-LC3B WT) B
            amHI SalI [1136  nt] : (BamHI[4] / SalI[1144])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        26..130
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="bla" - label=AmpR promoter - 26..130 \ 
     CDS             131..991
                     /label=confers resistance to ampicillin, carbenicillin, and_AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="bla" - product="beta-lactamase" - label=AmpR - note="confers resistance to ampicillin, carbenicillin, and - related antibiotics" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQ \ 
     primer_bind     complement(349..368)
                     /label=Ampicillin resistance gene, reverse primer_Amp-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Amp-R - note="Ampicillin resistance gene, reverse primer" - complement(349..368) \ 
     rep_origin      1162..1750
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of _ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=ori - note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  - replication" - 1162..1750 \ 
     primer_bind     1651..1670
                     /label=pBR322 origin, forward primer_pBR322ori-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pBR322ori-F - note="pBR322 origin, forward primer" - 1651..1670 \ 
     primer_bind     1904..1921
                     /label=L4440 vector, forward primer_L4440
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=L4440 - note="L4440 vector, forward primer" - 1904..1921 \ 
     protein_bind    2038..2059
                     /label=CAP binding activates transcription in the presence _CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CAP binding site - bound_moiety="E. coli catabolite activator protein" - note="CAP binding activates transcription in the presence  - of cAMP." - 2038..2059 \ 
     promoter        2074..2104
                     /label=promoter for the E. coli lac operon_lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac promoter - note="promoter for the E. coli lac operon" - 2074..2104 \ 
     protein_bind    2112..2128
                     /label=The lac repressor binds to the lac operator to _lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac operator - bound_moiety="lac repressor encoded by lacI" - note="The lac repressor binds to the lac operator to  - inhibit transcription in E. coli. This inhibition can be  - relieved by adding lactose or  - isopropyl-beta-D-thiogalactopyranoside \ 
     primer_bind     2117..2139
                     /label=In lacZ gene_M13/pUC Reverse
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13/pUC Reverse - note="In lacZ gene" - 2117..2139 \ 
     primer_bind     2136..2152
                     /label=common sequencing primer, one of multiple similar _M13 rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 rev - note="common sequencing primer, one of multiple similar  - variants" - 2136..2152 \ 
     primer_bind     2136..2152
                     /label=In lacZ gene. Also called M13-rev_M13 Reverse
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 Reverse - note="In lacZ gene. Also called M13-rev" - 2136..2152 \ 
     primer_bind     2171..2191
                     /label=T3 promoter, forward primer_T3
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T3 - note="T3 promoter, forward primer" - 2171..2191 \ 
     promoter        2173..2191
                     /label=promoter for bacteriophage T3 RNA polymerase_T3 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T3 promoter - note="promoter for bacteriophage T3 RNA polymerase" - 2173..2191 \ 
     promoter        2219..2445
                     /label=Rous sarcoma virus enhancer/promoter_RSV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=RSV promoter - note="Rous sarcoma virus enhancer/promoter" - 2219..2445 \ 
     LTR             2446..2626
                     /label=truncated 5' long terminal repeat (LTR) from HIV-1_5' LTR (truncated)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=5' LTR (truncated) - note="truncated 5' long terminal repeat (LTR) from HIV-1" - 2446..2626 \ 
     LTR             7742..7922
                     /label=truncated 5' long terminal repeat (LTR) from HIV-1_5' LTR (truncated)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=5' LTR (truncated) - note="truncated 5' long terminal repeat (LTR) from HIV-1" - 2446..2626 \ 
     misc_feature    2673..2798
                     /label=packaging signal of human immunodeficiency virus _HIV-1 Psi
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=HIV-1 Psi - note="packaging signal of human immunodeficiency virus  - type 1" - 2673..2798 \ 
     misc_feature    3291..3524
                     /label=The Rev response element (RRE) of HIV-1 allows for _RRE
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=RRE - note="The Rev response element (RRE) of HIV-1 allows for  - Rev-dependent mRNA export from the nucleus to the  - cytoplasm." - 3291..3524 \ 
     misc_feature    4020..4137
                     /label=central polypurine tract and central termination _cPPT/CTS
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cPPT/CTS - note="central polypurine tract and central termination  - sequence of HIV-1" - 4020..4137 \ 
     promoter        4183..4693
                     /label=human phosphoglycerate kinase 1 promoter_hPGK promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=hPGK promoter - note="human phosphoglycerate kinase 1 promoter" - 4183..4693 \ 
     primer_bind     4612..4630
                     /label=Human PGK promoter, forward primer_hPGK-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=hPGK-F - note="Human PGK promoter, forward primer" - 4612..4630 \ 
     primer_bind     complement(4753..4774)
                     /label=EGFP, reverse primer_EGFP-N
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=EGFP-N - note="EGFP, reverse primer" - complement(4760..4781) \ 
     primer_bind     complement(5014..5033)
                     /label=For distinguishing EGFP variants, reverse primer_EXFP-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=EXFP-R - note="For distinguishing EGFP variants, reverse primer" - complement(5021..5040) \ 
     primer_bind     5361..5382
                     /label=EGFP, forward primer_EGFP-C
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=EGFP-C - note="EGFP, forward primer" - 5368..5389 \ 
     misc_feature    5830..6418
                     /label=woodchuck hepatitis virus posttranscriptional _WPRE
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=WPRE - note="woodchuck hepatitis virus posttranscriptional  - regulatory element" - 5450..6038 \ 
     primer_bind     complement(5883..5903)
                     /label=WPRE, reverse primer_WPRE-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=WPRE-R - note="WPRE, reverse primer" - complement(5503..5523) \ 
     CDS             complement(6301..6312)
                     /label=Factor Xa site
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - product="Factor Xa recognition and cleavage site" - label=Factor Xa site - translation="IEGR" - complement(5921..5932) \ 
     primer_bind     complement(6467..6490)
                     /label=Murine stem cell virus, reverse primer_MSCV-rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=MSCV-rev - note="Murine stem cell virus, reverse primer" - complement(6087..6110) \ 
     primer_bind     6864..6883
                     /label=Mouse PGK promoter, forward primer_mPGK-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=mPGK-F - note="Mouse PGK promoter, forward primer" - 6484..6503 \ 
     CDS             6952..7551
                     /label=confers resistance to puromycin_PuroR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="pac from Streptomyces alboniger" - product="puromycin N-acetyltransferase" - label=PuroR - note="confers resistance to puromycin" - translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER - VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAG \ 
     primer_bind     complement(6952..6971)
                     /label=Puromycin resistance gene, reverse primer. Also _Puro-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Puro-R - note="Puromycin resistance gene, reverse primer. Also  - called puro-variant-R" - complement(6572..6591) \ 
     primer_bind     7448..7468
                     /label=Puromycin resistance gene, forward primer_Puro-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Puro-F - note="Puromycin resistance gene, forward primer" - 7068..7088 \ 
     LTR             7689..7922
                     /label=self-inactivating 3' long terminal repeat (LTR) from_3' LTR (Delta-U3)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=3' LTR (Delta-U3) - note="self-inactivating 3' long terminal repeat (LTR) from - HIV-1" - 7309..7542 \ 
     polyA_signal    7994..8115
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 poly(A) signal - note="SV40 polyadenylation signal" - 7614..7735 \ 
     primer_bind     complement(8031..8050)
                     /label=SV40 polyA, reverse primer_SV40pA-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40pA-R - note="SV40 polyA, reverse primer" - complement(7651..7670) \ 
     primer_bind     8085..8104
                     /label=SV40 polyA terminator, reverse primer_EBV-rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=EBV-rev - note="SV40 polyA terminator, reverse primer" - 7705..7724 \ 
     rep_origin      8155..8290
                     /label=SV40 origin of replication_SV40 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 ori - note="SV40 origin of replication" - 7775..7910 \ 
     primer_bind     8217..8236
                     /label=SV40 promoter/origin, forward primer_SV40pro-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40pro-F - note="SV40 promoter/origin, forward primer" - 7837..7856 \ 
     primer_bind     complement(8310..8329)
                     /label=T7 promoter, forward primer_T7
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 - note="T7 promoter, forward primer" - complement(7930..7949) \ 
     promoter        complement(8311..8329)
                     /label=promoter for bacteriophage T7 RNA polymerase_T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 promoter - note="promoter for bacteriophage T7 RNA polymerase" - complement(7931..7949) \ 
     primer_bind     complement(8339..8356)
                     /label=In lacZ gene. Also called M13-F20 or M13 (-21) _M13 Forward
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 Forward - note="In lacZ gene. Also called M13-F20 or M13 (-21)  - Forward" - complement(7959..7976) \ 
     primer_bind     complement(8339..8355)
                     /label=common sequencing primer, one of multiple similar _M13 fwd
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 fwd - note="common sequencing primer, one of multiple similar  - variants" - complement(7959..7975) \ 
     primer_bind     complement(8348..8370)
                     /label=In lacZ gene_M13/pUC Forward
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13/pUC Forward - note="In lacZ gene" - complement(7968..7990) \ 
     rep_origin      8497..8952
                     /label=f1 bacteriophage origin of replication; arrow _f1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=f1 ori - note="f1 bacteriophage origin of replication; arrow  - indicates direction of (+) strand synthesis" - 8117..8572 \ 
     primer_bind     complement(8584..8603)
                     /label=F1 origin, reverse primer_F1ori-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1ori-R - note="F1 origin, reverse primer" - complement(8204..8223) \ 
     primer_bind     8794..8815
                     /label=F1 origin, forward primer_F1ori-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1ori-F - note="F1 origin, forward primer" - 8414..8435 \ 
                     4701..5472
                     /label=insert from PCR(lentipuro-EGFP-LC3B WT) BamHI SalI [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(lentipuro-EGFP-LC3B WT) BamHI SalI [1136  nt] : (BamHI[4] / SalI[1144]) \ 
                     5473..5824
                     /label=insert from PCR(lentipuro-EGFP-LC3B WT) BamHI SalI [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(lentipuro-EGFP-LC3B WT) BamHI SalI [1136  nt] : (BamHI[4] / SalI[1144]) \ 
     misc_feature    4708..5424
                     /label=EGFP
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS \ 
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc 
     4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct 
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 
     4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt 
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 
     4681 cctctctccc cagggggatc CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG 
     4741 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC 
     4801 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC 
     4861 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC 
     4921 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA 
     4981 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC 
     5041 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC 
     5101 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC 
     5161 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC 
     5221 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC 
     5281 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC 
     5341 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT 
     5401 CACGGCATGG ACGagctCTA CAAAAGCGGT TCCGGACCGG ATCAAACAAG TTTGTACAAA 
     5461 AAAGCAGGCT TAATGAAGTT CCAGTACAAG GAGGACCATC CCTTTGAGTA TCGGAAAAAG 
     5521 GAAGGAGAAA AGATCCGGAA GAAATATCCG GACAGGGTCC CCGTGATTGT AGAGAAGGCT 
     5581 CCAAAAGCCA GGGTGCCTGA TCTGGACAAG AGGAAGTACC TAGTGCCCTC TGACCTTACT 
     5641 GTTGGCCAGT TCTACTTCTT AATCCGGAAG AGAATCCACC TGAGACCTGA GGACGCCTTA 
     5701 TTCTTCTTTG TCAACAACAC CATCCCTCCC ACCAGTGCTA CCATGGGCCA ACTGTATGAG 
     5761 GACAATCATG AGGAAGACTA TTTTCTGTAT GTGGCCTACA GTGATGAGAG TGTCTATGGG 
     5821 TAAgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 
     5881 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 
     5941 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 
     6001 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 
     6061 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 
     6121 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 
     6181 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 
     6241 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 
     6301 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 
     6361 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 
     6421 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 
     6481 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 
     6541 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 
     6601 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 
     6661 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 
     6721 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 
     6781 gctgggaagg ggtgggtccg ggggnnggct caggggcggg ctcaggggcg gggcgggcgc 
     6841 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 
     6901 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 
     6961 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 
     7021 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 
     7081 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 
     7141 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 
     7201 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 
     7261 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 
     7321 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 
     7381 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 
     7441 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 
     7501 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc 
     7561 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt 
     7621 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     7681 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac 
     7741 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     7801 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     7861 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     7921 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     7981 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     8041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     8101 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     8161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     8221 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     8281 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     8341 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     8401 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     8461 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     8521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     8581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     8641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     8701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     8761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     8821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     8881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     8941 cgcttacaat tt
//