LOCUS       pHalo-Tomm20-N-1        2108 bp    DNA     linear       08-FEB-2019
"Contig 1" (1,2109)
  Contig Length:                 2109 bases
  Average Length/Sequence:        799 bases
  Total Sequence Length:         3997 bases  Top Strand:                       3 sequences
  Bottom Strand:                    2 sequences
  Total:                            5 sequences
FEATURES             Location/Qualifiers
     contig          1..2108
                     /Note="Contig 1(1>2109)"
                     /dnas_scaffold_ID="0"
                     /dnas_scaffold_POS="0"
     coverage_once   1..124
                     /Note="Only_once"
     coverage_below  125..813
                     /Note="Below threshold"
     coverage_one    814..908
                     /Note="One_strand"
     coverage_below  909..1650
                     /Note="Below threshold"
     coverage_once   1651..2108
                     /Note="Only_once"
     misc_feature    631..1524
                     /note="Halo Tag"
     unsure          601..630
                     /note="linker 10 AA"
     misc_feature    166..600
                     /note="Tomm20"
     source          1..2108
                     /dnas_title="pHalo-Tomm20-N-10"
ORIGIN      
        1 CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT
       61 CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
      121 ATAAGCAGAG CTGGTTTAGT GACCGTCAGA TCCGCTAGCG CCACCATGGT GGGTCGGAAC
      181 AGCGCCATCG CCGCCGGTGT ATGCGGGGCC CTTTTCATTG GGTACTGCAT CTACTTCGAC
      241 CGCAAAAGAC GAAGTGACCC CAACTTCAAG AACAGGCTTC GAGAACGAAG AAAGAAACAG
      301 AAGCTTGCCA AGGAGAGAGC TGGGCTTTCC AAGTTACCTG ACCTTAAAGA TGCTGAAGCT
      361 GTTCAGAAGT TCTTCCTTGA AGAAATACAG CTTGGTGAAG AGTTACTAGC TCAAGGTGAA
      421 TATGAGAAGG GCGTAGACCA TCTGACAAAT GCAATTGCTG TGTGTGGACA GCCACAGCAG
      481 TTACTGCAGG TCTTACAGCA AACTCTTCCA CCACCAGTGT TCCAGATGCT TCTGACTAAG
      541 CTCCCAACAA TTAGTCAGAG AATTGTAAGT GCTCAGAGCT TGGCTGAAGA TGATGTGGAA
      601 GGCGGTAGCG GGGATCCACC GGTCGCCACC ATGGCAGAAA TCGGTACTGG CTTTCCATTC
      661 GACCCCCATT ATGTGGAAGT CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC
      721 GATGGCACCC CTGTGCTGTT CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC
      781 ATCATCCCGC ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC
      841 AAATCCGACA AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT CATGGATGCC
      901 TTCATCGAAG CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA TTCACGACTG GGGCTCCGCT
      961 CTGGGTTTCC ACTGGGCCAA GCGCAATCCA GAGCGCGTCA AAGGTATTGC ATTTATGGAG
     1021 TTCATCCGCC CTATCCCGAC CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG
     1081 GCCTTCCGCA CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG
     1141 GGTACGCTGC CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA CCATTACCGC
     1201 GAGCCGTTCC TGAATCCTGT TGACCGCGAG CCACTGTGGC GCTTCCCAAA CGAGCTGCCA
     1261 ATCGCCGGTG AGCCAGCGAA CATCGTCGCG CTGGTCGAAG AATACATGGA CTGGCTGCAC
     1321 CAGTCCCCTG TCCCGAAGCT GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC
     1381 GAAGCCGCTC GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT
     1441 CTGAATCTGC TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC GCGCTGGCTG
     1501 TCGACGCTCG AGATTTCCGG CTAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC
     1561 ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA
     1621 TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
     1681 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
     1741 TTTGTCCAAA CTCATCAATG TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA
     1801 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA
     1861 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA
     1921 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
     1981 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC
     2041 GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC
     2101 CGGCGAAC
//

