LOCUS Exported 6493 bp ds-DNA circular UNA 12-FEB-2019 DEFINITION natural circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 6493) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 12, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6493 /organism="unspecified" /mol_type="genomic DNA" unsure 1..141 /label=L-ITR /note="/vntifkey=21" promoter 156..597 /label=synapsin primer_bind 484..503 /label=synapsin-seq-fwd CDS 598..2595 /codon_start=1 /label=GRAB-NE1h /label=GRAB-NE2.1 /label=GRAB-NE2.2 /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGGSLQPDAGNASWN GTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLV SLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRY WSITQAIEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEIN DQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRP NGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNG IKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMV LLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEG EGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY IQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARW RGRQNREKRFKFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSL NPVIYTIFNHDFRRAFKKILCRGDRKRIVL" unsure 2610..3218 /label=WPRE /note="/vntifkey=21" primer_bind complement(2695..2712) /label=pWPTRev polyA_signal 3238..3716 /label=hGH polyA /label=hGH polyA /label=PolyA signal /note="/vntifkey=25" unsure 3756..3896 /label=R-ITR /note="/vntifkey=21" rep_origin 3988..4294 /label=f1 Origin /label=f1 Origin /label=Replication origin /note="/vntifkey=33" unsure 4813..5670 /label=AmpR /note="/vntifkey=21" rep_origin 5821..6488 /label=pUC ori /label=pUC ori /label=Replication origin /note="/vntifkey=33" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc 181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc 241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt 301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca 361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg 421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg 481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg 541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg 601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac 661 acaagtttgt acaaaaaagt tggcaccacc ggtggctccc tgcagccgga cgcgggcaac 721 gcgagctgga acgggaccga ggcgccgggg ggcggcgccc gggccacccc ttactccctg 781 caggtgacgc tgacgctggt gtgcctggcc ggcctgctca tgctgctcac cgtgttcggc 841 aacgtgctcg tcatcatcgc cgtgttcacg agccgcgcgc tcaaggcgcc ccaaaacctc 901 ttcctggtgt ctctggcctc ggccgacatc ctggtggcca cgctcgtcat ccctttctcg 961 ctggccaacg aggtcatggg ctactggtac ttcggcaagg cttggtgcga gatctacctg 1021 gcgctcgacg tgctcttctg cacgtcgtcc atcgtgcacc tgtgcgccat cagcctggac 1081 cgctactggt ccatcacaca ggccatcgag tacaacctga agcgcacgcc gcgccgcatc 1141 aaggccatca tcatcaccgt gtgggtcatc tcggccgtca tctccttccc gccgctcatc 1201 tccatcgaga agaagggcgg cggcggcggc ccgcagccgg ccgagccgcg ctgcgagatc 1261 aacgaccaga agtggtacgt catctcgtcg tgcatcggct ccttcttcgc tccctgcctc 1321 atcatgatcc tggtctacgt gcgcatctac cagatcgcca agcgtcgcac ccgcgtgcca 1381 cccagccgcc ggggtccgga cgccgtcgcc gcgccgccgg ggggcaccga gcgcaggccc 1441 aacggtctgg gccccgagcg cagcgcgggc ccggggggcg cagaggccga accgctgccc 1501 acccagctca acggcgcccc tggcgagccc gcgccggccg ggccgcgcga caccgacgcg 1561 ctggacctgg aggagggagg aaacgtctat atcaaggccg acaagcagaa gaacggcatc 1621 aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac 1681 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 1741 agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg 1801 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc 1861 ggagggagca tggtgagaaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 1921 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat 1981 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 2041 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 2101 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc 2161 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 2221 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 2281 ctggggcaca agctggagta caacaccgga gcagcagcac gctggcgcgg gcggcagaac 2341 cgcgagaagc gcttcaagtt cgtgctggcc gtggtcatcg gagtgttcgt ggtgtgctgg 2401 ttccccttct tcttcaccta cacgctcacg gccgtcgggt gctccgtgcc acgcacgctc 2461 ttcaaattct tcttctggtt cggctactgc aacagctcgt tgaacccggt catctacacc 2521 atcttcaacc acgatttccg ccgcgccttc aagaagatcc tctgtcgggg ggacaggaag 2581 cggatcgtgt tgtgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa 2641 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 2701 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 2761 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 2821 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 2881 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 2941 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 3001 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt 3061 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 3121 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 3181 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg 3241 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 3301 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 3361 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 3421 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 3481 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 3541 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 3601 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 3661 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 3721 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc 3781 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg 3841 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg 3901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 3961 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 4021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 4081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 4141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca 4201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 4261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct 4321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 4381 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc 4441 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 4501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 4561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 5521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6481 tttgctcaca tgt //