LOCUS       Exported                6493 bp ds-DNA     circular UNA 12-FEB-2019
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     primer_bind     484..503
                     /label=synapsin-seq-fwd
     CDS             598..2595
                     /codon_start=1
                     /label=GRAB-NE1h
                     /label=GRAB-NE2.1
                     /label=GRAB-NE2.2
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGGSLQPDAGNASWN
                     GTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLV
                     SLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRY
                     WSITQAIEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEIN
                     DQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRP
                     NGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNG
                     IKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEG
                     EGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
                     IQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARW
                     RGRQNREKRFKFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSL
                     NPVIYTIFNHDFRRAFKKILCRGDRKRIVL"
     unsure          2610..3218
                     /label=WPRE
                     /note="/vntifkey=21"
     primer_bind     complement(2695..2712)
                     /label=pWPTRev
     polyA_signal    3238..3716
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3756..3896
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3988..4294
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4813..5670
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5821..6488
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtggctccc tgcagccgga cgcgggcaac
      721 gcgagctgga acgggaccga ggcgccgggg ggcggcgccc gggccacccc ttactccctg
      781 caggtgacgc tgacgctggt gtgcctggcc ggcctgctca tgctgctcac cgtgttcggc
      841 aacgtgctcg tcatcatcgc cgtgttcacg agccgcgcgc tcaaggcgcc ccaaaacctc
      901 ttcctggtgt ctctggcctc ggccgacatc ctggtggcca cgctcgtcat ccctttctcg
      961 ctggccaacg aggtcatggg ctactggtac ttcggcaagg cttggtgcga gatctacctg
     1021 gcgctcgacg tgctcttctg cacgtcgtcc atcgtgcacc tgtgcgccat cagcctggac
     1081 cgctactggt ccatcacaca ggccatcgag tacaacctga agcgcacgcc gcgccgcatc
     1141 aaggccatca tcatcaccgt gtgggtcatc tcggccgtca tctccttccc gccgctcatc
     1201 tccatcgaga agaagggcgg cggcggcggc ccgcagccgg ccgagccgcg ctgcgagatc
     1261 aacgaccaga agtggtacgt catctcgtcg tgcatcggct ccttcttcgc tccctgcctc
     1321 atcatgatcc tggtctacgt gcgcatctac cagatcgcca agcgtcgcac ccgcgtgcca
     1381 cccagccgcc ggggtccgga cgccgtcgcc gcgccgccgg ggggcaccga gcgcaggccc
     1441 aacggtctgg gccccgagcg cagcgcgggc ccggggggcg cagaggccga accgctgccc
     1501 acccagctca acggcgcccc tggcgagccc gcgccggccg ggccgcgcga caccgacgcg
     1561 ctggacctgg aggagggagg aaacgtctat atcaaggccg acaagcagaa gaacggcatc
     1621 aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac
     1681 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1741 agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1801 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1861 ggagggagca tggtgagaaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1921 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat
     1981 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2041 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2101 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc
     2161 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2221 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2281 ctggggcaca agctggagta caacaccgga gcagcagcac gctggcgcgg gcggcagaac
     2341 cgcgagaagc gcttcaagtt cgtgctggcc gtggtcatcg gagtgttcgt ggtgtgctgg
     2401 ttccccttct tcttcaccta cacgctcacg gccgtcgggt gctccgtgcc acgcacgctc
     2461 ttcaaattct tcttctggtt cggctactgc aacagctcgt tgaacccggt catctacacc
     2521 atcttcaacc acgatttccg ccgcgccttc aagaagatcc tctgtcgggg ggacaggaag
     2581 cggatcgtgt tgtgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2641 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2701 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2761 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2821 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2881 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2941 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3001 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     3061 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3121 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3181 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
     3241 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     3301 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     3361 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     3421 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     3481 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     3541 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     3601 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     3661 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     3721 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3781 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3841 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3961 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     4321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4381 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4441 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6481 tttgctcaca tgt
//
