LOCUS       Exported                7662 bp ds-DNA     circular DNA 12-FEB-2019
DEFINITION  pcDNA3.1/Puro-CAG-ASAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="pcDNA3.1/Puro-CAG-ASAP1"
                     /mol_type="other DNA"
     primer_bind     1..33
                     /label=pcDNA3.1-ArchT-F
     gene            14..1486
                     /label=ArchT-BFP2
     primer_bind     699..716
                     /label=ArchT-seq-f
     primer_bind     744..792
                     /label=ArchT-egfp-f
     primer_bind     complement(753..772)
                     /label=ArchT-r-forEGFP
     gene            773..1486
                     /label=bfp2
     unsure          1470..1600
                     /label=pcDNA3.1-ArchT-r
     primer_bind     complement(1472..1523)
                     /label=TS+ERex-rv1
     gene            1487..1570
                     /label=TS+ERex
     primer_bind     complement(1509..1563)
                     /label=TS+ERex-rv2
     primer_bind     complement(1547..1600)
                     /label=TS+ERex-rv3
     primer_bind     1571..1590
                     /label=pcDNA3.1-linear-f
     primer_bind     complement(1575..1590)
                     /label=pcDNA3.1-linear-f
     misc_feature    complement(1685..1702)
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     terminator      1688..1915
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     rep_origin      1978..2284
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    complement(2398..2418)
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    complement(2404..2619)
                     /label=SV40_enhancer
                     /label=SV40_enhancer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     promoter        2416..2684
                     /label=SV40_promoter
                     /label=SV40_promoter
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     rep_origin      2583..2660
                     /label=SV40_origin
                     /label=SV40_origin
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     misc_feature    2645..2664
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     gene            2741..3340
                     /gene="puro (variant)"
                     /label=puro (variant)
                     /label=puro (variant)
                     /note="/translation=METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQ
                     DSCSN 
                     TIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFF 
                     LVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDLAAFNSHNVYITADK 
                     QKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK 
                     RDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSV 
                     RGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSA 
                     MPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNT 
                     DQMPQMVTLLRVLRIVILIRIFRLASQKKQLEVVT*"
     CDS             2741..3340
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     terminator      3503..3622
                     /label=SV40_PA_terminator
                     /label=SV40_PA_terminator
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3591..3610
                     /label=EBV_rev_primer
                     /label=EBV_rev_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     primer_bind     complement(3648..3667)
                     /label=SV40polyA-2
     promoter        complement(3666..3684)
                     /label=M13_reverse_primer
                     /label=M13_reverse_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    complement(3683..3705)
                     /label=M13_pUC_rev_primer
                     /label=M13_pUC_rev_primer
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     promoter        complement(3719..3748)
                     /label=lac_promoter
                     /label=lac_promoter
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     primer_bind     4131..4148
                     /label=f-xa.7
     primer_bind     complement(4133..4150)
                     /label=f-xa.J
     primer_bind     4577..4594
                     /label=f-xa.9
     gene            complement(4828..5688)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDP
                     DRHIE 
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR 
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL 
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             complement(4828..5688)
                     /codon_start=1
                     /label=ORF frame 1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5039..5056
                     /label=f-xa.11
     primer_bind     complement(5045..5062)
                     /label=f-xa.E
     promoter        complement(5730..5758)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    5919..6206
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             complement(6575..7183)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPARH
                     GEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     APRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPAQRA
                     LPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE"
     misc_feature    7518..7537
                     /label=pCAG_F_primer
                     /label=pCAG_F_primer
                     /note="/translation=MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLP
                     PPPAR 
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA 
                     QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
     primer_bind     7649..7662
                     /label=pcDNA3.1-ArchT-F (2)
     primer_bind     complement(7656..7662)
                     /label=pcDNA3.1-linear-r
                     /label=nonstandard type: primer
     primer_bind     complement(join(7662,1..1550))
                     /label=TS+ERex-rv2
                     /label=nonstandard type: primer
ORIGIN
        1 gctagccgcc accatggacc ccatcgctct gcaggcagga tacgacctgc tgggcgacgg
       61 aaggccagag accctgtggc tgggaatcgg aaccctgctg atgctgatcg gcaccttcta
      121 cttcatcgtg aagggctggg gcgtgaccga caaggaggcc agggagtact acagcatcac
      181 aatcctggtg cccggcatcg ccagcgccgc ctacctgagc atgttcttcg gcatcggcct
      241 gaccgaggtg accgtggccg gcgaggtgct ggacatctac tacgccagat acgccgactg
      301 gctgttcacc acccctttgc ttctgctcga cctggccctg ctggctaagg tggacagggt
      361 gagcatcgga accctggtgg gagtggacgc cctgatgatc gtgaccggcc tgatcggcgc
      421 cctgagccac accccactgg ctaggtacag ctggtggctg ttcagcacca tctgcatgat
      481 cgtggtgctg tacttcctgg ctaccagcct gagggctgct gctaaggaga ggggaccaga
      541 ggtggctagc accttcaaca ccctgaccgc cctggtgctg gtgctgtgga ccgcctaccc
      601 catcctgtgg atcatcggaa ccgagggagc tggagtggtg ggactgggaa tcgagaccct
      661 gctgttcatg gtgctggacg tgaccgccaa agtgggcttc ggcttcatcc tgctgaggag
      721 cagggccatc ctgggcgaca ccgaggcccc cgagccccga ccggtagtag cagtgagcaa
      781 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      841 cggccacaag ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac
      901 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      961 cctgagccac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     1021 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1081 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1141 cgagctgaag ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1201 caacttcaac agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt
     1261 gaacttcaag atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca
     1321 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac
     1381 ccagtccgtg ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1441 ccgcaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaaaa gtagaatcac
     1501 aagcgaaggc gagtacatcc ccctggatca aatagacata aatgtaggtg gattttgtta
     1561 tgagaatgaa tgaaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     1621 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     1681 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1741 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1801 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1861 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     1921 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     1981 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2041 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2101 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2161 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2221 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2281 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2341 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     2401 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2461 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2521 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2581 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2641 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     2701 gaggcttttt tggaggccta ggcttttgca aaaagctccc atgaccgagt acaagcccac
     2761 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     2821 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     2881 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     2941 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3001 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3061 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3121 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3181 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3241 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3301 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttcgaaatga ccgaccaagc
     3361 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3421 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3481 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     3541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3601 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     3661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     4621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5821 cgtcgacgga tcggggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg
     5881 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     5941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6061 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6121 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     6181 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     6241 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     6301 aattattttg tgcagcgatg ggggcggggg gggggggcgc gcgccaggcg gggcggggcg
     6361 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     6421 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     6481 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     6541 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     6601 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     6661 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     6721 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     6781 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     6841 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     6901 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     6961 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     7021 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     7081 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     7141 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     7201 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     7261 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     7321 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     7381 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     7441 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     7501 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     7561 attcctcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     7621 ggccaatgcc ctggctcaca aataccactg agatctgcat ga
//
