LOCUS       pLC-Flag-CSNK1A1        8156 bp    DNA     circular UNA 19-DEC-2017
DEFINITION  CSNK1A1 isoform2.
ACCESSION   urn.local...1500501653185.10
VERSION     urn.local...1500501653185.10
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6459..7493
                     /note="Geneious type: ORF"
                     /label="CK1a isoform2"
     gene            2244..3104
                     /label="Gene_1"
     misc_recomb     37..70
                     /label="LoxP"
     misc_feature    3237^3238
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    3449..4053
                     /note="Geneious type: promoter prokaryotic"
                     /label="CMV promoter/enhancer"
     misc_feature    5861..6451
                     /note="Geneious type: promoter uukaryotic"
                     /label="CMV"
     rep_origin      1426..2099
                     /label="pUC"
     LTR             734..1423
                     /label="3' SIN-LTR"
     LTR             4072..4746
                     /label="5' LTR"
     primer_bind     6444..6503
                     /label=3661
     primer_bind     5831..5881
                     /label=3592
     misc_feature    complement(6444..6503)
                     /note="Geneious type: primer_bind_reverse"
                     /label=3660
     misc_feature    complement(7472..7518)
                     /note="Geneious type: primer_bind_reverse"
                     /label=3734
     misc_feature    2244..3104
                     /label="AmpR"
     misc_feature    4776..5633
                     /label="Psi"
     misc_feature    7560..8156
                     /label="Puro"
     misc_feature    125..714
                     /label="WRE"
     misc_feature    5659..5836
                     /label="FLAP"
     misc_feature    7494..7559
                     /label="P2A peptide"
     misc_feature    5831..5860
                     /label="Gibson arm"
     misc_feature    7494..7518
                     /label="Gibson arm"
     misc_feature    6462..6485
                     /label="Flag"
     misc_feature    complement(7472..7493)
                     /label="Primer anneal"
     misc_feature    5861..5881
                     /label="Primer anneal"
     misc_feature    5713..5732
                     /label="2667 FLAP SeqF"
     misc_feature    complement(86..104)
                     /label=258
     misc_feature    complement(7633..7649)
                     /label="3059 Puro seq R"
     misc_feature    6453..6458
                     /label="kozak sequence"
     misc_feature    6..13
                     /note="Geneious type: restriction site"
                     /label="NotI"
     misc_feature    7691..7697
                     /note="Geneious type: restriction site"
                     /label="BstEII"
     misc_feature    18..23
                     /note="Geneious type: restriction site"
                     /label="EcoRI"
     misc_feature    5855..5860
                     /note="Geneious type: restriction site"
                     /label="BamHI"
     misc_feature    3468..3473
                     /note="Geneious type: restriction site"
                     /label="MluI"
     misc_feature    7494..7499
                     /note="Geneious type: restriction site"
                     /label="BamHI"
     misc_feature    2684..2689
                     /note="Geneious type: restriction site"
                     /label="PvuI"
     misc_feature    732..737
                     /note="Geneious type: restriction site"
                     /label="PvuI"
     misc_feature    7930..7935
                     /note="Geneious type: restriction site"
                     /label="BalI"
     misc_feature    7886..7891
                     /note="Geneious type: restriction site"
                     /label="StuI"
     misc_feature    724..729
                     /note="Geneious type: restriction site"
                     /label="SalI"
     misc_feature    3238..3243
                     /note="Geneious type: restriction site"
                     /label="SalI"
     misc_feature    2795..2800
                     /note="Geneious type: restriction site"
                     /label="ScaI"
     misc_feature    715..720
                     /note="Geneious type: restriction site"
                     /label="ClaI"
     misc_feature    120..125
                     /note="Geneious type: restriction site"
                     /label="ClaI"
     misc_feature    852..857
                     /note="Geneious type: restriction site"
                     /label="KpnI"
     misc_feature    4449..4454
                     /note="Geneious type: restriction site"
                     /label="NruI"
     misc_feature    3446..3451
                     /note="Geneious type: restriction site"
                     /label="NruI"
     misc_feature    3828..3833
                     /note="Geneious type: restriction site"
                     /label="SnaBI"
     misc_feature    6199..6204
                     /note="Geneious type: restriction site"
                     /label="SnaBI"
     misc_feature    3401..3406
                     /note="Geneious type: restriction site"
                     /label="MfeI"
     misc_feature    4809..4814
                     /note="Geneious type: restriction site"
                     /label="MfeI"
     misc_feature    881..886
                     /note="Geneious type: restriction site"
                     /label="PvuII"
     misc_feature    7044..7049
                     /note="Geneious type: restriction site"
                     /label="NheI"
     misc_feature    6094..6099
                     /note="Geneious type: restriction site"
                     /label="NdeI"
     misc_feature    3723..3728
                     /note="Geneious type: restriction site"
                     /label="NdeI"
     misc_feature    638..643
                     /note="Geneious type: restriction site"
                     /label="NaeI"
     misc_feature    4764..4769
                     /note="Geneious type: restriction site"
                     /label="NaeI"
     misc_feature    6961..6966
                     /note="Geneious type: restriction site"
                     /label="BalI"
     misc_feature    6510..6515
                     /note="Geneious type: restriction site"
                     /label="EcoRI"
     misc_feature    7240..7245
                     /note="Geneious type: restriction site"
                     /label="PstI"
     misc_feature    5653..5658
                     /note="Geneious type: restriction site"
                     /label="PstI"
ORIGIN      
        1 aatatgcggc cgccagagaa ttcgtcgagg gacctaataa cttcgtatag catacattat
       61 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
      121 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      181 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      241 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      301 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      361 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      421 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
      481 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
      541 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
      601 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
      661 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
      721 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
      781 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
      841 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
      901 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
      961 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     1021 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     1081 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     1141 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     1201 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     1261 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1321 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1381 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     1441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3181 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3241 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     3301 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     3361 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     3421 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     3481 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3541 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3601 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3661 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3721 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3781 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3841 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     3901 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3961 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4021 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     4081 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4141 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4201 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     4261 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     4321 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     4381 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     4441 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     4501 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     4561 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     4621 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     4681 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     4741 gtaagaccac cgcacagcaa gcggccggcc gcgctgatct tcagacctgg aggaggagat
     4801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     4861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     4921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     4981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     5041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     5101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     5161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     5221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     5281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     5341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     5401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     5521 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     5581 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     5641 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     5701 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     5761 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     5821 cagagatcca gtttggttag taccgggccc gagaggatcc ttagttatta atagtaatca
     5881 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5941 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6001 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6061 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6121 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6181 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6241 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6301 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6361 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6421 agcagagctg gtttagtgaa ccgtcagatc cggccaccat ggattacaaa gacgatgacg
     6481 ataaggcgag tagcagcggc tccaaggctg aattcattgt cggagggaaa tataaactgg
     6541 tacggaagat cgggtctggc tccttcgggg acatctattt ggcgatcaac atcaccaacg
     6601 gcgaggaagt ggcagtgaag ctagaatctc agaaggccag gcatccccag ttgctgtacg
     6661 agagcaagct ctataagatt cttcaaggtg gggttggcat cccccacata cggtggtatg
     6721 gtcaggaaaa agactacaat gtactagtca tggatcttct gggacctagc ctcgaagacc
     6781 tcttcaattt ctgttcaaga aggttcacaa tgaaaactgt acttatgtta gctgaccaga
     6841 tgatcagtag aattgaatat gtgcatacaa agaattttat acacagagac attaaaccag
     6901 ataacttcct aatgggtatt gggcgtcact gtaataagtt attccttatt gattttggtt
     6961 tggccaaaaa gtacagagac aacaggacaa ggcaacacat accatacaga gaagataaaa
     7021 acctcactgg cactgcccga tatgctagca tcaatgcaca tcttggtatt gagcagagtc
     7081 gccgagatga catggaatca ttaggatatg ttttgatgta ttttaataga accagcctgc
     7141 catggcaagg gctaaaggct gcaacaaaga aacaaaaata tgaaaagatt agtgaaaaga
     7201 agatgtccac gcctgttgaa gttttatgta aggggtttcc tgcagaattt gcgatgtact
     7261 taaactattg tcgtgggcta cgctttgagg aagccccaga ttacatgtat ctgaggcagc
     7321 tattccgcat tcttttcagg accctgaacc atcaatatga ctacacattt gattggacaa
     7381 tgttaaagca gaaagcagca cagcaggcag cctcttccag tgggcagggt cagcaggccc
     7441 aaacccccac aggcaagcaa actgacaaaa ccaagagtaa catgaaaggt ttcggatccg
     7501 gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga
     7561 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7621 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7681 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7741 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7801 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7861 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7921 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7981 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8041 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8101 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctga
//
