LOCUS pcDNA3-Venus-cpVenus-FLARE-AKAR 7494 bp DNA circular 2/8/19 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=Degen. DNA COMMENT SerialCloner_Comments=Ligation of : pc'AKAR4.xdna [6765 nt] : (SphI[1727] / BamHI [1002]) to Untitled Sequence #1 [721 nt] : (BamHI[41] / Sph I[766]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2200..2223 /label=PKAsub /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1735..2157 /label=FHA1 /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 2248..2982 /label=cpVenus-E172 /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1009..1725 /label=Venus /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(5333..5353) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(3047..3064) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(5721..6349) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(5359..5381) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(6501..7160) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4202..4993 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(7400..7428) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(5386..5415) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3983..4060 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 3087..3314 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GGTGAGCAAG 1021 GGCGAGGAGC TGTTCACCGg ggtGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 1081 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 1141 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 1201 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 1261 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 1321 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 1381 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 1441 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 1501 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG 1561 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC 1621 CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 1681 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGCGcat gcataagttt 1741 tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt 1801 cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag 1861 aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga 1921 ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac 1981 atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta 2041 ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg 2101 gtcattttca taaacgacaa atttaagcag tgcctcgagc agaacaaagt tgatcgctct 2161 gcaggtaagc caggcagcgg cgagggcagc accaagggcc tgcgtcgcgc caccctggtg 2221 gacggcggca ccggcggcag cgagctcatg ggcggcgtgc agctcgccga ccactaccag 2281 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 2341 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 2401 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggagg taccggtgga 2461 tctatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 2521 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 2581 tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc 2641 accctcgtga ccaccctggg ctacggcctt cagtgcttcg cccgctaccc cgaccacatg 2701 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 2761 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 2821 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 2881 cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag 2941 aacggcatca aggccaactt caagatccgc cacaacatcg agtaaGAATT CTGCAGATAT 3001 CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCTATTctat agtgtcacct 3061 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 3121 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 3181 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 3241 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 3301 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 3361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 3421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 3481 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 3541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 3601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 3661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 3721 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 3781 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3841 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3961 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4081 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4141 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 4201 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4261 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4321 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4381 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4441 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4501 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4561 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4621 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4681 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 4741 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4801 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4861 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4921 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4981 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 5041 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 5101 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 5161 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 5221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 5281 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 5341 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 5401 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 5461 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 5521 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 5581 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5641 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5701 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5761 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5821 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5881 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 5941 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6001 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6061 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6121 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6181 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6241 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6301 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6361 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6421 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6481 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6541 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6601 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6661 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6721 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6781 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 6841 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 6901 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 6961 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7021 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7081 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7141 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7201 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7261 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7321 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7381 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 7441 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //