LOCUS pcDNA3-Cer3-Cer3-FLARE-AKAR 7476 bp DNA circular 2/14/19 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=Degen. DNA COMMENT SerialCloner_Comments=Ligation of : pcDNA3-Cer3-cpCer3-FLARE-AKAR.gb.xdna [6746 n t] : (EcoRI[2983] / SacI[2242]) to Untitled Sequence #1 [722 nt] : (SacI[306] / EcoRI[1032]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2200..2223 /label=PKAsub /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1735..2157 /label=FHA1 /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1009..1725 /label=CER3 /SerialCloner_Color=&h66CCFF /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2248..2964 /label=CER3 /SerialCloner_Color=&h66CCFF /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(5315..5335) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(3029..3046) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(5703..6331) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(5341..5363) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(6483..7142) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4184..4975 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(7382..7410) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(5368..5397) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3965..4042 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 3069..3296 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GGTGAGCAAG 1021 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 1081 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 1141 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 1201 CTGAGCTGGG GCGTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 1261 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 1321 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 1381 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 1441 AACGCCATCC ACGGCAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 1501 AACTTCGGCC TCAACTGCAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 1561 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 1621 CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 1681 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGCGcat gcataagttt 1741 tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt 1801 cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag 1861 aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga 1921 ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac 1981 atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta 2041 ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg 2101 gtcattttca taaacgacaa atttaagcag tgcctcgagc agaacaaagt tgatcgctct 2161 gcaggtaagc caggcagcgg cgagggcagc accaagggcc tgcgtcgcgc caccctggtg 2221 gacggcggca ccggcggcag cgagctCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG 2281 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC 2341 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC 2401 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGAGCTGGGG CGTGCAGTGC 2461 TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA 2521 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC 2581 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC 2641 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACGCCATCCA CGGCAACGTC 2701 TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCGGCCT CAACTGCAAC 2761 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC 2821 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCAAGCT GAGCAAAGAC 2881 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT 2941 CTCGGCATGG ACGAGCTGTA CAAGTAAGaa ttCTGCAGAT ATCCATCACA CTGGCGGCCG 3001 CTCGAGCATG CATCTAGAGG GCCCTATTct atagtgtcac ctaaatgcta gagctcgctg 3061 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 3121 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 3181 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 3241 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 3301 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 3361 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3421 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3481 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 3541 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3601 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3661 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 3721 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 3841 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 3961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 4021 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 4081 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 4141 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 4201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 4261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 4381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4681 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 4741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 4801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 4861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 4921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 4981 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 5041 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 5101 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 5161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 5221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 5281 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 5341 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 5401 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 5461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 5521 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5881 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 5941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6121 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 6421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 6481 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 6541 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 6601 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 6661 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 6721 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 6781 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 6841 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 6901 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 6961 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7021 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7081 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7201 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7261 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7321 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 7381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 7441 ccgcgcacat ttccccgaaa agtgccacct gacgtc //