LOCUS       pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(TA)    7572 bp      DNA     circular   2/14/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-Venus-cpVenus-FLARE-EKAR-EV.gb.xdna [74
            99  nt] : (SacI[2320] / Kpn2I[2251]) to Untitled Sequence #1
             [65  nt] : (Kpn2I[222] / SacI[291])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1903..2250
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2257..2310
                     /label=ERKsub_TA
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1735..1896
                     /label=WW_Domain
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2326..3060
                     /label=cpVenus-E172
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1009..1725
                     /label=Venus
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5411..5431)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3125..3142)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5799..6427)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5437..5459)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6579..7238)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4280..5071
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7478..7506)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5464..5493)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4061..4138
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3165..3392
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GGTGAGCAAG 
     1021 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 
     1081 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 
     1141 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 
     1201 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 
     1261 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 
     1321 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 
     1381 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 
     1441 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 
     1501 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG 
     1561 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC 
     1621 CAGTCCAAGC TGAGCAAAGA cCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 
     1681 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAgCTGT ACAAGCGcat gCACATGGCG 
     1741 GACGAGGAGA AGCTGCCGCC CGGCTGGGAG AAGCGCATGA GCCGCAGCTC AGGCCGAGTG 
     1801 TACTACTTCA ACCACATCAC TAACGCCAGC CAGTGGGAGC GGCCCAGCGG CAACAGCAGC 
     1861 AGTGGTGGCA AAAACGGGCA GGGGGAGCCT GCCAGGGGTA CCAGTGCTGG TGGTAGTGCT 
     1921 GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT AGTGCTGGTG GTTCCGGCAG TGCTGGTGGT 
     1981 AGTGCTGGTG GTAGTACCAG TGCTGGTGGT AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT 
     2041 GGTGGTAGTG CTGGTGGTTC CGGCAGTGCT GGTGGTAGTG CTGGTGGTAG TACCAGTGCT 
     2101 GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT AGTGCTGGTG GTAGTGCTGG TGGTTCCGGC 
     2161 AGTGCTGGTG GTAGTGCTGG TGGTAGTACC AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT 
     2221 GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT TccggACCAG ATGTCCCTAG AGCCCCAGTG 
     2281 GATAAAGCAA AGCTGTCATT CCAATTCCCC CGCGCACGGG agctCATGGG CGGCGTGCAG 
     2341 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC 
     2401 AACCACTACc TGAGCTACCA GTCCAAGCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC 
     2461 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC 
     2521 AAGGGAGGTA CCGGTGGATC TATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG 
     2581 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG 
     2641 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG 
     2701 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTTCA GTGCTTCGCC 
     2761 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 
     2821 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 
     2881 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 
     2941 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC 
     3001 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGAG 
     3061 TAAGaattCT GCAGATATCC ATCACACTGG CGGCCGCTCG AGCATGCATC TAGAGGGCCC 
     3121 TATTctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag 
     3181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 
     3241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 
     3301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 
     3361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 
     3421 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     3481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     3541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc 
     3601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     3661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     3721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     3781 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct 
     3841 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 
     3901 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     4021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 
     4081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     4141 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     4201 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 
     4261 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 
     4321 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 
     4381 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 
     4441 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 
     4501 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 
     4561 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 
     4621 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 
     4681 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 
     4741 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 
     4801 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 
     4861 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 
     4921 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 
     4981 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 
     5041 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 
     5101 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 
     5161 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 
     5221 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa 
     5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     5341 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc 
     5401 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     5461 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     5521 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     5581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     5641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     5701 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     5761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     5821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     5881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     6001 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     6061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     6121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     6181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     6241 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     6301 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     6361 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     6421 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     6481 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     6541 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     6601 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     6661 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     6721 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     6781 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     6841 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     6901 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     6961 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     7021 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     7081 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     7141 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     7201 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     7261 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     7321 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     7381 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     7441 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     7501 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     7561 ccacctgacg tc
//