LOCUS       pcDNA3-Lyn-AktAR    7461 bp      DNA     circular   12/12/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-Lyn-AKAR4.gb.xdna [5443  nt] : (EcoRI[2
            923] / BamHI[939]) to pcDNA3-AktAR.gb.xdna [2010  nt] : (Bam
            HI[1002] / EcoRI[3016])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     907..939
                     /label=Lyn-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2215..2952
                     /label=cpV-E172
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MGGVQLADHYQQNTPIGDGPVLLXDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE*" \ 
     misc_feature    946..1662
                     /label=CER
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(5300..5320)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(3014..3031)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(5688..6316)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(5326..5348)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(6468..7127)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             4169..4960
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(7367..7395)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(5353..5382)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3950..4027
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      3054..3281
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1672..2094
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2146..2190
                     /label=AKTsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PRPRSCTWPDPRPEF" \ 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCaa gcttGCGGCC 
      901 GCCACCATGG GCTGCATCAA GAGCAAGCGC AAGGACAAgg atcccatggt gagcaagggc 
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 
     1021 cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 
     1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 
     1141 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 
     1201 aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa ggacgacggc 
     1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 
     1381 gccatcagtg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcccac 
     1441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 
     1501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 
     1561 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 
     1621 accgccgccg ggatcactct cggcatggac gagctgtaca agcgcatgca taagttttct 
     1681 caagaacaga tcggcgaaaa cattgtgtgc agggtcattt gtaccacggg tcaaattccc 
     1741 atccgagatt tgtcagctga tatttcacaa gtgcttaagg aaaaacgatc cataaagaaa 
     1801 gtttggacat ttggtagaaa cccagcctgt gactatcatt taggaaacat ttcaagactg 
     1861 tcaaataagc atttccaaat actactagga gaagacggta accttttatt gaatgacatt 
     1921 tccactaatg ggacctggtt aaatgggcaa aaagtcgaga agaacagcaa tcagttactg 
     1981 tctcaaggtg atgaaataac cgttggtgta ggcgtggaat cagatatttt atctctggtc 
     2041 attttcataa acgacaaatt taagcagtgc ctcgagcaga acaaagttga tcgctctgca 
     2101 ggtaagccag gcagcggcga gggcagcacc aagggcctgg tcgaccctcg tccgcgctcg 
     2161 tgcacctggc cggaccccag gccggagttt ggaggtaccg gcggcagcga gctcatgggc 
     2221 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 
     2281 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag 
     2341 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 
     2401 gagctgtaca agggaggtac cggtggatct atggtgagca agggcgagga gctgttcacc 
     2461 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 
     2521 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc 
     2581 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggccttcag 
     2641 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 
     2701 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 
     2761 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 
     2821 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 
     2881 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 
     2941 aacatcgagt aaGaattCTG CAGATATCCA TCACACTGGC GGCCGCTCGA GCATGCATCT 
     3001 AGAGGGCCCT ATTctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt 
     3061 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     3121 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     3181 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     3241 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 
     3301 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 
     3361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     3421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     3481 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     3541 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     3601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     3661 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa 
     3721 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 
     3781 gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa 
     3841 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     3901 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     3961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     4021 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg 
     4081 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca 
     4141 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 
     4201 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 
     4261 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 
     4321 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 
     4381 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 
     4441 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 
     4501 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 
     4561 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 
     4621 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 
     4681 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 
     4741 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 
     4801 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 
     4861 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 
     4921 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 
     4981 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     5041 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     5101 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 
     5161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     5221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     5281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     5881 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     6121 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     7441 cgaaaagtgc cacctgacgt c
//