LOCUS       Exported                2931 bp ds-DNA     circular SYN 15-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 15, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [305 nt] : (Uncut 5'[0] / Uncut 3'[305])
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..720)
                     /label=US71_APikL1
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [305 nt] : (Uncut 5'[0] / Uncut 3'[305])"
     primer_bind     complement(755..771)
                     /label=M13Rv
     CDS             complement(1916..2731)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatgag aaaagataat gaaattatga gaaaaggtgg tattgatttg tctagagaac
      481 cagatccaaa ttttattgat catgctggta ctagaccagc tgaatgtttt tggtttatgt
      541 attataatgg tcaaagaatt gatgctggta cttgttattc tgcttggtct tttggtttga
      601 aagttggttc ttcttttgtt gatattaaaa ctgatgaaga ttgtaatttg tatggtactt
      661 atgctccagg ttggattgtt ttgggtaaaa aaagaccagg tttttgagct tagagaccaa
      721 atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct gtgtgaaatt
      781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttaga
     1921 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     1981 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     2041 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     2101 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     2161 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     2221 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     2281 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     2341 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     2401 ctaatacctg gaatgctgtt ttcccaggga tcgcagtggt gagtaaccat gcatcatcag
     2461 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     2521 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     2581 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     2641 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     2701 agcaagacgt ttcccgttga atatggctca tactcttcct ttttcaatat tattgaagca
     2761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2881 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
