LOCUS       Exported                2988 bp ds-DNA     circular SYN 15-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 15, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [362 nt] : (Uncut 5'[0] / Uncut 3'[362])
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..777)
                     /label=CD156_APikL3
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [362 nt] : (Uncut 5'[0] / Uncut 3'[362])"
     primer_bind     complement(812..828)
                     /label=M13Rv
     CDS             complement(1973..2788)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatgaa aattgaaaat gctattgttc caggttttag aattgatttg tctagagaac
      481 caaatccagt tgtttatgat gctccaggtt ctccagttca agaatgtttt tggtttatgt
      541 tttataatgg tcaaagagtt gatgctagag cttgtcattc tggttggtct tttggtttgc
      601 aagttggtaa aagatttgtt caaattaaaa ctgataaaaa atttaatttg catggtactt
      661 atgctccagg ttggattgtt ttgggtagag aaagagatga tttgaaaaat aatcaaaatc
      721 aaagaatgca aggtaaaact attactacta gagaaggtaa ttgagcttag agaccaaatc
      781 ccaatggcgc gccgagcttg gctcgagcat ggtcatagct gtttcctgtg tgaaattgtt
      841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1621 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttagaaaa
     1981 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     2041 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     2101 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     2161 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     2221 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     2281 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     2341 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     2401 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     2461 atacctggaa tgctgttttc ccagggatcg cagtggtgag taaccatgca tcatcaggag
     2521 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     2581 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     2641 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     2701 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     2761 aagacgtttc ccgttgaata tggctcatac tcttcctttt tcaatattat tgaagcattt
     2821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2941 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
