LOCUS       Exported                2853 bp ds-DNA     circular SYN 15-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 15, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [227 nt] : (Uncut 5'[0] / Uncut 3'[227])
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..642)
                     /label=AVR-Pia
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [227 nt] : (Uncut 5'[0] / Uncut 3'[227])"
     primer_bind     complement(677..693)
                     /label=M13Rv
     CDS             complement(1838..2653)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatggc tccagctaga ttttgtgttt attatgatgg tcatttgcca gctactagag
      481 ttttgttgat gtatgttaga attggtacta ctgctactat tactgctaga ggtcatgaat
      541 ttgaagttga agctaaagat caaaattgta aagttatttt gactaatggt aaacaagctc
      601 cagattggtt ggctgctgaa ccatattgag cttagagacc aaatcccaat ggcgcgccga
      661 gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1801 caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca
     1861 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     1921 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     1981 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     2041 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     2101 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     2161 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     2221 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     2281 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     2341 ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     2401 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     2461 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2521 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     2581 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
     2641 gaatatggct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2761 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2821 ataaaaatag gcgtatcacg aggccctttc gtc
//
