LOCUS       Exported                8206 bp ds-DNA     circular SYN 15-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 15, 2019 from SnapGene 4.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    263..826
                     /label=pU6 (pMJ177) 5' CMV promoter IE
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    complement(766..775)
                     /label=GC Box
                     /note="ApEinfo_fwdcolor=#84b0dc
                     ApEinfo_revcolor=#84b0dc"
     misc_feature    844..1024
                     /label=HIV-1_5_LTR
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     misc_feature    complement(1601..1607)
                     /label=TATA box
                     /note="ApEinfo_fwdcolor=#ff9ccd
                     ApEinfo_revcolor=#ff9ccd"
     misc_feature    1695..1928
                     /label=RRE
                     /note="ApEinfo_fwdcolor=#b7e6d7
                     ApEinfo_revcolor=#b7e6d7"
     misc_feature    2459..2473
                     /label=cPPT
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     misc_feature    2631..2944
                     /label=mU6 promoter (modified)
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_recomb     2825..2858
                     /label=LoxP recombination site
                     /note="ApEinfo_fwdcolor=#75c6a9
                     ApEinfo_revcolor=#75c6a9"
     misc_feature    2919
                     /label=mU6 promoter (modified)
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    2934..2936
                     /label=mU6 promoter (modified)
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    2940..2944
                     /label=mU6 promoter (modified)
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    3015..3597
                     /label=CMV promoter IE
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    complement(3518..3527)
                     /label=GC Box
                     /note="ApEinfo_fwdcolor=#84b0dc
                     ApEinfo_revcolor=#84b0dc"
     misc_feature    3625..4329
                     /label=mCherry
                     /note="ApEinfo_fwdcolor=#800000
                     ApEinfo_revcolor=#800000"
     CDS             4339..4395
                     /codon_start=1
                     /label=P2A
                     /note="ApEinfo_fwdcolor=#faac61
                     ApEinfo_revcolor=#faac61"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4396..4716
                     /label=DD
                     /note="ApEinfo_fwdcolor=#993366
                     ApEinfo_revcolor=#993366"
     misc_feature    4726..4986
                     /label=acrIIA4
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     misc_recomb     5006..5039
                     /label=LoxP recombination site
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     misc_feature    5085..5685
                     /label=wPRE
                     /note="ApEinfo_fwdcolor=#0060ff
                     ApEinfo_revcolor=#0060ff"
     misc_feature    5875..5889
                     /label=cPPT
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     misc_feature    6212..6392
                     /label=HIV-1_5_LTR
                     /note="ApEinfo_fwdcolor=#85dae9
                     ApEinfo_revcolor=#85dae9"
     rep_origin      complement(6436..7118)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor=#9eafd2
                     ApEinfo_revcolor=#9eafd2"
     misc_feature    complement(6439..7058)
                     /label=pBR322_origin
                     /note="ApEinfo_fwdcolor=#b4abac
                     ApEinfo_revcolor=#b4abac"
     misc_feature    complement(6463..6472)
                     /label=GC Box
                     /note="ApEinfo_fwdcolor=#84b0dc
                     ApEinfo_revcolor=#84b0dc"
     CDS             complement(7216..7875)
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
     misc_feature    complement(8115..8143)
                     /label=AmpR promoter
                     /note="ApEinfo_fwdcolor=#ffef86
                     ApEinfo_revcolor=#ffef86"
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggcgcgc catcagtctc gagtactagg atccattagg cggccgcgtg gataaccgta
     3001 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     3061 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3361 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3421 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3481 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3541 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3601 atccgctagc ccaccatgcc cggggtgagc aagggcgagg aggataacat ggccatcatc
     3661 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3721 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3781 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3841 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3901 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3961 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4021 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4081 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4141 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4201 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4261 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4321 ctgtacaagg gttctggagc tactaacttc tccctgctga aacaggcagg agacgtggag
     4381 gagaaccctg gaccaggagt gcaagtggaa accatctccc caggagacgg gcgcaccttc
     4441 cccaagcgcg gccagacctg tgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
     4501 gtcgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
     4561 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
     4621 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc
     4681 actctcgtct tcgatgtgga gcttctaaaa cctgaaggat ctggaaacat caacgatctg
     4741 atccgggaga tcaagaacaa ggactacacc gtgaagctga gcggcaccga tagcaacagc
     4801 atcacccagc tgattatccg cgtgaacaac gacggcaacg agtacgtgat ctccgagagc
     4861 gagaacgaga gcatcgtgga gaagttcatc agcgccttca agaacggttg gaaccaggag
     4921 tacgaggacg aggaggagtt ctacaacgac atgcagacca tcaccctgaa gagcgagctg
     4981 aactaagaat tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata
     5041 catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa
     5101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5701 cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5761 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5821 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5881 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5941 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6001 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6061 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6121 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     6181 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     6241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6361 acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
