LOCUS       Exported                8804 bp ds-DNA     circular SYN 18-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pF420
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   James Antoney
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 18, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="recombinant plasmid"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     terminator      60..103
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    106..127
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     106..126
                     /label=SB4K5_ins_fwd
     primer_bind     complement(106..126)
                     /label=BBa_RFC10_Vec_Rev
                     /note="Primer for amplifying BioBrick plasmids for Gibson 
                     assembly"
     primer_bind     106..126
                     /label=BBa_RFC10_Fwd
                     /note="Primer for amplifying BioBricks"
     primer_bind     106..124
                     /label=BBa_G1004
     regulatory      128..180
                     /regulatory_class="promoter"
                     /note="TetR repressible promoter"
     protein_bind    128..146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      189..219
                     /regulatory_class="ribosome_binding_site"
                     /note="Optimised with RBS calculator v2.0
                     TIR = 1165154.12"
     RBS             207..215
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             220..1128
                     /codon_start=1
                     /gene="cofD"
                     /locus_tag="MM_RS09715"
                     /product="EPPG:Fo phosphoenolpyruvyl transferase"
                     /EC_number="2.7.8.28"
                     /label=CofD
                     /note="Codon optimised for E. coli"
                     /protein_id="WP_011033809.1"
                     /translation="MIIFSGGTGTPKLLDGLKEILPEEELTVVVNTAEDLWVSGNLISP
                     DLDTVLYLFSDQIDRKRWWGIENDTFGTYERMKELGIEEGLKLGDRDRATHIIRSNIIR
                     DGASLTDSTVKLSSLFGIKANILPMSDDPVSTYIETAEGIMHFQDFWIGKRGEPDVRGV
                     DIRGVSEASISPKVLEAFEKEENILIGPSNPITSIGPIISLPGMRELLKKKKVVAVSPI
                     IGNAPVSGPAGKLMPACGIEVSSMGVAEYYQDFLDVFVFDERDRADEFAFERLGCHASR
                     ADTLMTSTEKSKELAEIVVQAF"
     primer_bind     complement(672..691)
                     /label=U4965CK070-6-SEQ0.5r
                     /note="Sequencing primer"
     primer_bind     complement(672..691)
                     /label=U4965CK070-6-SEQ0.5r
     regulatory      1132..1174
                     /regulatory_class="ribosome_binding_site"
                     /note="Optimised with RBS calculator v2.0
                     TIR = 798485.52"
     RBS             1161..1169
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1175..2536
                     /codon_start=1
                     /gene="fbiB"
                     /locus_tag="MSMEG_1892"
                     /product="gamma-glutamate ligase"
                     /EC_number="6.3.2.31"
                     /label=FbiB
                     /note="Codon optimised for E. coli"
                     /protein_id="WP_011727937.1"
                     /translation="MSAAANAEHGAADRVEILPVPGLPEFRPGDDLVGSLAEAAPWLRD
                     GDVLVVTSKVVSKCEGRIVAAPSDPEERDTLRRKLIDDEAVRVLARKGRTLITENAIGL
                     VQAAAGVDGSNVGSTELALLPVDPDRSAATLREGLRERLGVTVGVVITDTMGRAWRTGQ
                     TDFAIGASGLTVLQGYAGSRDRHGNELVVTEVAVADEIAAAADLVKGKLTAIPVAVVRG
                     LRLPDDGSTAHRLVRAGEDDLFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPHDAIEA
                     AVGEALTAPAPHHTRPVRFVWVQDSETRTRLLDRMKEQWRADLTADGLDADAVDRRVAR
                     GQILYDAPELVIPFLVPDGAHSYPDDARTAAEHTMFTVAVGAAVQGLLVALAVRDIGSC
                     WIGSTIFAADLVRAELELPDDWEPLGAIAIGYPEQTPQPLGPRDPVPTDELLVRK"
     primer_bind     complement(1305..1324)
                     /label=U4965CK070-6-SEQ1R
                     /note="Sequencing primer"
     primer_bind     complement(1305..1324)
                     /label=U4965CK070-6-SEQ1R
     primer_bind     1899..1918
                     /label=U4965CK070-6-SEQ3
                     /note="Sequencing primer"
     primer_bind     1899..1918
                     /label=U4965CK070-6-SEQ3
     primer_bind     complement(2020..2039)
                     /label=U4965CK070-6-SEQ2R
                     /note="Sequencing primer"
     primer_bind     complement(2020..2039)
                     /label=U4965CK070-6-SEQ2R
     regulatory      2540..2591
                     /regulatory_class="ribosome_binding_site"
                     /note="Optimised with RBS calculator v2.0
                     TIR = 772868.81"
     RBS             2578..2586
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2592..3254
                     /codon_start=1
                     /gene="fbiD"
                     /locus_tag="MSMEG_2392"
                     /product="phosphoenolpyruvate guanylyltransferase"
                     /EC_number="2.7.7.68"
                     /label=FbiD
                     /note="Codon optimised for E. coli"
                     /protein_id="WP_003893758.1"
                     /translation="MSGRRTPGAASDGSAGGQAAVIIAVKRLTAAKTRLAPIFSAATRE
                     EVVLAMLVDTITAASTVAPVTVVTPDEVAADAARQLGAGVLADPTPEGHHNPLNNAIMA
                     AEEHLRAGTPNIVVLQGDLPAMQPRELAEALAAARTYPRSFVGDRHGTGTSALFAFGVP
                     LQPRFGADSATHHRHSGAIELTGAWPGLRCDIDTAEDLRTARRLGVGPATAQAIAAHR"
     primer_bind     2615..2634
                     /label=U4965CK070-6-SEQ4
                     /note="Sequencing primer"
     primer_bind     2615..2634
                     /label=U4965CK070-6-SEQ4
     regulatory      3258..3298
                     /regulatory_class="ribosome_binding_site"
                     /note="Optimised with RBS calculator v2.0
                     TIR = 132509.97"
     primer_bind     3284..3303
                     /label=U4965CK070-6-SEQ5
                     /note="Sequencing primer"
     primer_bind     3284..3303
                     /label=U4965CK070-6-SEQ5
     CDS             3299..5920
                     /codon_start=1
                     /gene="fbiC"
                     /locus_tag="MSMEG_5126"
                     /product="FO Synthase"
                     /label=FbiC
                     /note="Codon optimised for E. coli"
                     /protein_id="YP_889372.1"
                     /translation="MRRVDESRLSWSYSGVALNSEHGADLGVPLVPPPAVTPSPAALRR
                     VLRRARDGVALNVDEAAIAMTARGDDLADLCASAARVRDAGLTTAGRRGPNGRLPVSYS
                     RKVFIPVTHLCRDTCHYCTFVTVPGKLRAQGMGMYMEPDEILDVARQGAELGCKEALFT
                     LGDRPEDRWEEARQWLDERGYDSTLDYVRAMAIRVLEETGLLPHLNPGVMSWSELSRLK
                     PVAPSMGMMLETTSRRLFETKGEAHYGSPDKDPAVRLRTLDDAGRLSIPFTTGLLVGIG
                     ETLAERAETMHAIRRSHKEFGHVQEVIVQNFRAKDRTAMASTPDADIDDFLATVAVTRL
                     VLGPKMRIQAPPNLVSRDECLALIGAGVDDWGGVSPLTPDHVNPERPWPALDELADVTA
                     EAGYDLVQRLTAQPQYVQAGAAWIDPRVRAHVDALADPDTGYALDVNPSGLPWQEPDEA
                     SESLGRTDLHTAIDAEGRLTDTRSDLDSAFGDWESIREKVSELAARAPERIDTDVLSAL
                     RSAERNPGGCSDDEYLALATADGPALEAVTALADSLRRDAVGDDVTFVVNRNINFTNIC
                     YTGCRFCAFAQRKGDADAYSLSVDEVADRAWEAHVAGATEVCMQGGIDPELPVTGYADL
                     VRAVKKRVPSMHVHAFSPMEIANGVTRSGMSIREWLTVLREAGLDTIPGTAAEILDDEV
                     RWVLTKGKLPTSMWIEVITTAHEVGLRSSSTMMYGHVDQPRHWVGHLNVIKSIQDRTGG
                     FTEFVPLPFVHQSSPLYLAGGSRPGPTHRDNRAVHALARIMLHGRIDHIQTSWVKLGIE
                     RTQVMLRGGANDLGGTLMEETISRMAGSENGSAKTVEELVAIAEGIGRPARQRTTTYAP
                     LAA"
     primer_bind     3937..3956
                     /label=U4965CK070-6-SEQ6N
                     /note="Sequencing primer"
     primer_bind     3937..3956
                     /label=U4965CK070-6-SEQ6N
     primer_bind     4348..4367
                     /label=U4965CK070-6-SEQ7
                     /note="Sequencing primer"
     primer_bind     4348..4367
                     /label=U4965CK070-6-SEQ7
     primer_bind     4868..4887
                     /label=U4965CK070-6-SEQ8
                     /note="Sequencing primer"
     primer_bind     4868..4887
                     /label=U4965CK070-6-SEQ8
     primer_bind     5481..5500
                     /label=U4965CK070-6-SEQ9
                     /note="Sequencing primer"
     primer_bind     5481..5500
                     /label=U4965CK070-6-SEQ9
     terminator      5932..5970
                     /label=BBa_B1006
                     /note="Terminator (artificial, large, %T~>90)
                     /product="
     RBS             6022..6033
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6040..6657
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     terminator      6741..6812
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6828..6855
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6862..6882
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(6862..6882)
                     /label=SB4K5_ins_rev
     primer_bind     6862..6882
                     /label=BBa_RCF10_Vec_Fwd
                     /note="Primer for amplifying BioBrick plasmids for Gibson 
                     assembly"
     primer_bind     complement(6862..6882)
                     /label=BBa_RCF10_Rev
                     /note="Primer for amplifying BioBricks"
     primer_bind     complement(6862..6882)
                     /label=BBa_G1005
     primer_bind     complement(6865..6877)
                     /label=BBa_G1004
     terminator      6883..6940
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(7016..7035)
                     /label=BBa_G1001_VR
                     /note="Sequencing primer for iGEM plasmids"
     rep_origin      complement(7135..7723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      7906..8000
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8021..8680)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     join(8792..8804,1..7)
                     /label=BBa_G1000_VF2
                     /note="Sequencing primer for iGEM plasmids"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
       61 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct
      121 tctagagtcc ctatcagtga tagagatgac atccctatca gtgatagaga tactgagcac
      181 tactagagcg aggccataat ttatattaag gaggttcata tgatcatttt tagcggtggt
      241 acaggcaccc cgaaactgct ggatggtctg aaagaaattc tgccggaaga agaactgacc
      301 gttgttgtta atacagccga agatctgtgg gttagcggta atctgattag tccggatctg
      361 gataccgttc tgtacctgtt tagcgatcag attgaccgta aacgttggtg gggtattgaa
      421 aatgatacct ttggcaccta tgaacgcatg aaagaactgg gcattgaaga gggtctgaaa
      481 ctgggtgatc gtgatcgtgc aacccatatc attcgtagca acattattcg tgatggtgcc
      541 agcctgaccg atagcaccgt taaactgagc agcctgtttg gtattaaagc aaatattctg
      601 ccgatgagtg atgatccggt tagcacctat attgaaacag cggaaggtat tatgcacttc
      661 caggattttt ggattggtaa acgtggtgaa ccggatgttc gtggtgttga tattcgcggt
      721 gttagcgaag caagcattag cccgaaagtt ctggaagcat ttgaaaaaga agagaacatt
      781 ctgattggtc cgagcaatcc gattaccagc atcggtccga ttattagcct gcctggtatg
      841 cgtgaactgc tgaaaaaaaa gaaagttgtt gcagtgagcc cgattattgg taatgcaccg
      901 gtgagcggtc cggcaggtaa actgatgcct gcatgtggta ttgaagttag cagcatgggt
      961 gttgccgaat attatcagga tttcctggat gtgtttgtgt ttgatgaacg tgatcgcgca
     1021 gatgaatttg catttgaacg tctgggttgt catgcaagcc gtgcagatac cctgatgacc
     1081 agcaccgaaa aaagcaaaga gctggcagaa attgttgttc aggcatttta atcggaataa
     1141 ctacacagac tacgaataac taaggaggta aaggatgagc gcagcagcaa atgcagaaca
     1201 tggtgcagca gatcgtgttg aaattctgcc ggttccgggt ctgccggaat ttcgtccggg
     1261 tgatgatctg gttggtagcc tggccgaagc agcaccgtgg ctgcgtgacg gtgatgtgct
     1321 ggttgttacc agcaaagttg ttagcaaatg tgaaggtcgt attgttgcag caccgagcga
     1381 tccggaagaa cgtgataccc tgcgtcgtaa actgattgat gatgaagcag ttcgtgttct
     1441 ggcacgtaaa ggtcgtaccc tgattaccga aaatgcaatt ggtctggttc aggcagcagc
     1501 cggtgttgat ggtagcaatg ttggtagcac cgaactggca ctgctgccgg ttgatcctga
     1561 tcgtagcgca gcaaccctgc gtgaaggtct gcgtgaacgc ctgggtgtta ccgttggtgt
     1621 tgttattacc gatacgatgg gtcgtgcatg gcgtaccggt cagaccgatt ttgccattgg
     1681 tgcaagcggt ctgacagttc tgcaaggtta tgcaggtagc cgtgatcgtc atggtaatga
     1741 actggttgtg accgaagttg cagttgcaga tgaaattgca gcagcagcgg atctggttaa
     1801 aggtaaactg accgcaattc cggttgccgt tgttcgtggt ctgcgcctgc cggatgatgg
     1861 tagtaccgca catcgtctgg ttcgtgccgg tgaagatgac ctgttttggc tgggcaccgc
     1921 agaagcaatt gaactgggtc gtcgtcaggc acagctgctg cgtcgttcag ttcgtcgttt
     1981 tagcgcagaa ccggtgccgc atgatgccat tgaagcagca gttggtgaag ccctgaccgc
     2041 accggcaccg catcataccc gtccggttcg ttttgtttgg gttcaggata gcgaaacccg
     2101 tacccgtctg ctggatcgta tgaaagaaca gtggcgtgca gatctgaccg cagatggtct
     2161 ggatgcagat gcagttgatc gtcgtgttgc acgtggtcag attctgtatg atgcaccgga
     2221 actggttatt ccgtttctgg ttccggatgg tgcacatagc tatccagatg atgcacgtac
     2281 cgcagcagaa cataccatgt ttaccgttgc agtgggtgca gccgttcagg gtctgctggt
     2341 tgcactggcc gttcgtgata ttggtagctg ttggattggt tcaaccattt ttgccgcaga
     2401 tctggttcgc gcagaactgg aactgcctga tgattgggaa ccgctgggtg caattgcgat
     2461 tggttatccg gaacagacac cgcagccgct gggtccgcgt gatccggttc cgaccgacga
     2521 actgctggtt cgtaaataaa tcagcaaaaa aggtaattag cataaaatta atattaataa
     2581 ggaggtaaaa catgagcggt cgtcgtacac cgggtgcagc aagtgatggt agtgccggtg
     2641 gtcaggcagc agttattatt gcagttaaac gtctgaccgc agcaaaaacc cgtctggcac
     2701 cgatttttag cgcagcaacc cgtgaagagg ttgttctggc aatgctggtt gataccatta
     2761 ccgcagccag caccgttgca ccggttaccg ttgttacacc ggatgaagtt gcagcagatg
     2821 cagcacgtca gctgggtgcc ggtgttctgg ccgatccgac accggaaggt catcataatc
     2881 cgctgaataa tgcaattatg gcagccgaag aacatctgcg tgcaggcacc ccgaatattg
     2941 ttgttctgca aggtgatctg cctgcaatgc agcctcgtga actggcagaa gcactggcag
     3001 cagcccgtac ctatccgcgt agctttgttg gtgatcgtca tggtacaggc accagcgcac
     3061 tgtttgcatt tggtgttccg ctgcaaccgc gttttggtgc agatagcgca acccatcatc
     3121 gtcatagcgg tgcaattgaa ctgaccggtg catggcctgg tctgcgttgt gatattgata
     3181 ccgcagaaga tctgcgtacc gcacgtcgtc tgggtgttgg tccggcaacc gcacaggcaa
     3241 ttgcagcaca tcgttaacgc cgccagtacc gagaataaat aactaaggag cccccataat
     3301 gcgtcgtgtt gatgaaagcc gtctgagctg gtcatatagc ggtgttgcac tgaatagcga
     3361 acatggtgca gatctgggtg ttccgctggt tccgcctccg gcagttaccc cgagtccggc
     3421 agcactgcgt cgcgttctgc gtcgtgcacg tgatggtgtg gccctgaatg tggatgaagc
     3481 agcaattgca atgaccgcac gtggtgatga tctggcagat ctgtgtgcaa gcgcagcacg
     3541 tgttcgtgat gcaggtctga ccaccgcagg tcgtcgtggt ccgaatggtc gtctgccggt
     3601 tagctatagc cgtaaagttt ttattccggt tacccatctg tgtcgtgata cctgtcatta
     3661 ttgtaccttt gttaccgttc cgggtaaact gcgtgcacag ggtatgggta tgtatatgga
     3721 accggatgaa attctggatg ttgcacgtca gggtgccgaa ctgggttgta aagaagcact
     3781 gtttaccctg ggtgatcgtc cggaagatcg ttgggaagag gcacgtcagt ggctggatga
     3841 acgtggttat gatagcaccc tggattatgt tcgtgcaatg gcaattcgtg ttctggaaga
     3901 aaccggtctg ctgccgcatc tgaatccggg tgttatgagt tggagcgaac tgagtcgtct
     3961 gaaaccggtt gcaccgagca tgggtatgat gctggaaacc accagtcgtc gtctgtttga
     4021 aaccaaaggt gaagcacatt atggtagtcc ggataaagat ccggcagtgc gtctgcgtac
     4081 cctggatgat gccggtcgtc tgagcattcc gtttaccaca ggtctgctgg ttggtattgg
     4141 tgaaaccctg gcagaacgtg cagaaaccat gcatgcaatt cgtcgtagcc ataaagaatt
     4201 tggtcatgtg caagaagtga tcgttcagaa ttttcgtgca aaagatcgta ccgcaatggc
     4261 cagcacaccg gatgcagata ttgatgattt tctggcaacc gttgccgtta cccgtctggt
     4321 tctgggtccg aaaatgcgta ttcaggcacc gcctaatctg gttagccgtg atgaatgtct
     4381 ggcactgatt ggtgccggtg tggatgattg gggtggtgtt agtccgctga caccggatca
     4441 tgttaatccg gaacgtccgt ggcctgcact ggacgaactg gcagatgtta cagccgaagc
     4501 aggttatgat ctggttcagc gtctgaccgc acagccgcag tatgttcagg caggcgcagc
     4561 atggattgat ccgcgtgtgc gtgcccatgt tgatgcactg gccgatccgg ataccggtta
     4621 tgcactggat gtgaatccga gcggtctgcc gtggcaagaa cctgatgaag caagcgaaag
     4681 cctgggtcgt accgatctgc ataccgcaat tgatgcagaa ggtcgcctga ccgatacccg
     4741 tagcgatctg gatagcgcat ttggtgattg ggaaagcatt cgtgaaaaag ttagcgagct
     4801 ggcagcccgt gcaccggaac gtattgatac cgatgttctg agcgcactgc gtagcgcaga
     4861 acgtaatcct ggtggttgta gtgatgatga gtatctggcc ctggccaccg cagatggtcc
     4921 ggcactggaa gcagttaccg cactggcgga tagcctgcgt cgggatgcag tgggtgatga
     4981 tgttaccttt gtggttaatc gcaacatcaa ctttaccaac atttgctata ccggttgtcg
     5041 tttttgtgca tttgcacagc gtaaaggtga tgcagatgca tatagcctga gcgttgatga
     5101 agttgcagat cgtgcatggg aagcacatgt tgccggtgcc accgaagttt gtatgcaggg
     5161 tggtattgat ccggaactgc cggtgaccgg ctatgccgat ctggtgcgtg cagttaaaaa
     5221 acgtgttccg agcatgcatg ttcatgcatt tagcccgatg gaaattgcaa atggtgttac
     5281 ccgtagtggt atgagtattc gtgaatggct gaccgtgctg cgtgaagccg gtctggatac
     5341 aattccgggt acagcagcag aaatcctgga tgacgaagtt cgttgggttc tgaccaaagg
     5401 caaactgccg accagcatgt ggattgaagt tattaccacc gcacatgaag ttggtctgcg
     5461 tagcagctca accatgatgt atggtcatgt tgatcagcct cgtcattggg ttggtcatct
     5521 gaatgtgatt aaaagcattc aggatcgcac cggtggtttt accgaatttg tgccgctgcc
     5581 gtttgttcat cagagcagtc cgctgtatct ggcaggcggt agccgtccgg gtccgaccca
     5641 tcgtgataat cgtgcagttc atgccctggc acgtattatg ctgcatggtc gcattgacca
     5701 tattcagacc agctgggtta aactgggtat tgaacgtacc caggtgatgc tgcgtggtgg
     5761 tgcaaatgat ctgggaggta cactgatgga agagacaatt agccgtatgg caggtagcga
     5821 aaatggtagc gcaaaaaccg ttgaagaact ggttgcgatt gccgaaggta ttggtcgtcc
     5881 ggcacgccag cgtaccacca cctacgcacc gctggcagca taatactaga gaaaaaaaaa
     5941 ccccgcccct gacagggcgg ggtttttttt tactagagtt gacagctagc tcagtcctag
     6001 ggactatgct agctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
     6061 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     6121 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     6181 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     6241 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     6301 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     6361 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     6421 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     6481 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     6541 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     6601 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
     6661 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
     6721 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     6781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     6841 gtgggccttt ctgcgtttat atactagtag cggccgctgc agtccggcaa aaaagggcaa
     6901 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
     6961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7141 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7561 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7801 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7861 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     7921 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     7981 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     8041 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     8101 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     8161 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     8221 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     8281 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     8341 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     8401 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     8461 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     8521 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     8581 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     8641 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     8701 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     8761 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgt
//
