LOCUS pSLHDR01_mCherry_tCD19 5868 bp ds-DNA circular 18-FEB-2019 DEFINITION . FEATURES Location/Qualifiers misc_feature 814..833 /label="Antisense" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 4791..4810 /label="Sense" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 4823..4847 /label="25 base overlap" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(2513..3220) /label="mCherry" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(4822..4841) /label="Antisense" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(1093..1113) /label="hU6-F" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature complement(4485..4490) /label="NruI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(1221..1423) /label="bGH-polyA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature complement(3254..4448) /label="hUbC promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature complement(3534..3535) /label="Modification" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(1170..4484) /label="Fragment c" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4811..4821 /label="Hairpin loop" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(834..844) /label="Hairpin loop" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(1457..2458) /label="tCD19" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(3526..3551) /label="Taqman probe" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature complement(1164..1169) /label="NruI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(845..864) /label="Sense" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature complement(1437..1456) /label="shGFP target" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature complement(2459..2512) /label="T2A" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature complement(3221..3225) /label="Kozak" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4462..4481 /label="2nd guide #1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(4460..4484) /label="25 base overlap" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 4920..4948 /label="Amp promoter" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(923..942) /label="LKO.1 5'" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 5188..5847 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(join(4491..5868,1..1163)) /label="Fragment a" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 4542..4782 /label="U6 promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature complement(4491..4515) /label="25 base overlap" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature complement(1173..1192) /label="2nd guide #2" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter complement(873..1113) /label="U6 promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind 4713..4732 /label="LKO.1 5'" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(1170..1194) /label="25 base overlap" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin 77..759 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 4542..4562 /label="hU6-F" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature complement(1139..1163) /label="25 base overlap" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 ORIGIN 1 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 61 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 121 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 181 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 241 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT 301 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 361 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 421 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 481 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 541 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 601 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 661 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 721 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 781 TGCTGGCCTT TTGCTCACAT GTGCGGCAAA AAAGCAAGCT GACCCTGAAG TTCGGACAGC 841 ACACGAACTT CAGGGTCAGC TTGCggtgtt tcgtcctttc cacaagatat ataaagccaa 901 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt 961 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat 1021 ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg 1081 caaatatgaa ggaatcatgg gaaataggcc ctcATTCTTG GGGTTGAGCT AAGGCAGGCT 1141 TGGGCAGAGG TGGAGGTGTA GAATCGCGAC CATACCCTGC GATGAGCGGC AGCGTACGCC 1201 ATAGAGCCCA CCGCATCCCC AGCATGCCTG CTATTGTCTT CCCAATCCTC CCCCTTGCTG 1261 TCCTGCCCCA CCCCACCCCC CAGAATAGAA TGACACCTAC TCAGACAATG CGATGCAATT 1321 TCCTCATTTT ATTAGGAAAG GACAGTGGGA GTGGCACCTT CCAGGGTCAA GGAAGGCACG 1381 GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGAAC 1441 TTCAGGGTCA GCTTGCTCAG AACCTCCTGG TGGGGTCGGT CATCCTCTTC CTCTTCCTCC 1501 TCAGCACCAG GGCCCTCTGC AGGTGCAGGA TGCCCACCAG GCTGCACAGG CAGAAGATCA 1561 GGTAGGCCAG GGTCACGGCG CTCACCTTCC AGCCGCCGGT CCTCAGCAGC CAGTGCCACA 1621 GCACGGGCCT GGCGGTGATC TCCAGGTGGA AGCTCATGGT CAGGTTGCCC CTGTGGCAGT 1681 AGTACTTGCC GGCGTCCTGG GCGGTGGCCC TGGGCAGCAG CAGGCCGGTC TCCATCACCC 1741 ACATGTCCCT GGCGGGCCTG TCGTCCTTCA GCTCCAGGCT CAGCAGGCTC TTGGGGCCCT 1801 TGGGGTGCAC GTGGGTCCAG CTCAGGGGGC CCCTGCTCAC GCTGTCGGGG GGCACGCCGC 1861 AGCTCAGCCA CAGGGTGCTG CCGGGGGCCA TGGTCAGGTC CTGGCTCAGG CTCTGGTTCA 1921 GGCTGTCCCT GGGGGGCAGG CAGGGGGGCT CGCCCTCCCA GATCTCGGGC CTGTCCTTGG 1981 CCCACACGTA CAGCTTGGGG CTCATCAGCT TGCCGCTGGG GCTGCTGGGG CCCTCGCTGC 2041 TCCTGTTCTT CAGGCCGCAG CCCAGGCCGC CCAGGTCGCT CACGTTCCAC CTGAACAGCT 2101 CGCCGCTGCC CTCCACGTTC ACGGTCCAGC CGGGCTGCCA GGCCTTCTCG CTGGGGGGGC 2161 CGGGCTGGCA CAGGTAGAAG CCGCCCATCT GCTGGCTCAC GTTGAAGATG AACAGCCAGA 2221 TGGCCAGGGG CCTCATGTGG ATGCCCAGGC CGGGCAGGCC CAGGCTCAGC TTCAGGAAGG 2281 GCTTCAGGGG GCTCTCCCTG CTCCAGGTCA GCTGCTGGGT GGGGCCGTCG CTGGTGCCCT 2341 TCAGGCACTG CAGCACGGCG TTGTCGCCCT CCTCCACCTT CACCACCAGG GGCTCCTCGG 2401 GCCTCACCTC CATGGGGGTC AGGAACAGCA GGAAGAACAG CAGCCTGGGG GGGGGCATGG 2461 GGCCGGGGTT CTCCTCCACG TCGCCGCAGG TCAGCAGGCT GCCCCTGCCC TCCTTGTACA 2521 GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC CCTCGGCGCG TTCGTACTGT TCCACGATGG 2581 TGTAGTCCTC GTTGTGGGAG GTGATGTCCA ACTTGATGTT GACGTTGTAG GCGCCGGGCA 2641 GCTGCACGGG CTTCTTGGCC TTGTAGGTGG TCTTGACCTC AGCGTCGTAG TGGCCGCCGT 2701 CCTTCAGCTT CAGCCTCTGC TTGATCTCGC CCTTCAGGGC GCCGTCCTCG GGGTACATCC 2761 GCTCGGAGGA GGCCTCCCAG CCCATGGTCT TCTTCTGCAT TACGGGGCCG TCGGAGGGGA 2821 AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA TGAACTCGCC GTCCTGCAGG GAGGAGTCCT 2881 GGGTCACGGT CACCACGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC TTGAAGCCCT 2941 CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG TAGGCCTTGG 3001 AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG CCACCCTTGG 3061 TCACCTTCAG CTTGGCGGTC TGGGTGCCCT CGTAGGGGCG GCCCTCGCCC TCGCCCTCGA 3121 TCTCGAACTC GTGGCCGTTC ACGGAGCCCT CCATGTGCAC CTTGAAGCGC ATGAACTCCT 3181 TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCGATG GATCCTTCGA 3241 AGAGCTCGGT ACCGTCTAAC AAAAAAGCCA AAAACGGCCA GAATTTAGCG GACAATTTAC 3301 TAGTCTAACA CTGAAAATTA CATATTGACC CAAATGATTA CATTTCAAAA GGTGCCTAAA 3361 AAACTTCACA AAACACACTC GCCAACCCCG AGCGCATAGT TCAAAACCGG AGCTTCAGCT 3421 ACTTAAGAAG ATAGGTACAT AAAACCGACC AAAGAAACTG ACGCCTCACT TATCCCTCCC 3481 CTCACCAGAG GTCCGGCGCC TGTCGATTCA GGAGAGCCTA CCCTAGGCCC GAATTCTGCG 3541 TCCTGCGACG GAGAAAAGCC TACCGCACAC CTACCGGCAG GTGGCCCCAC CCTGCATTAT 3601 AAGCCAACAG AACGGGTGAC GTCACGACAC GACGAGGGCG CGCGCTCCCA AAGGTACGGG 3661 TGCACTGCCC AACGGCACCG CCATAACTGC CGCCCCCGCA ACAGACGACA AACCGAGTTC 3721 TCCAGTCAGT GACAAACTTC ACGTCAGGGT CCCCAGATGG TGCCCCAGCC CATCTCACCC 3781 GAATAAGAGC TTTCCCGCAT TAGCGAAGGC CTCAAGACCT TGGGTTCTTG CCGCCCACCA 3841 TGCCCCCCAC CTTGTTTCAA CGACCTCACA GCCCGCCTCA CAAGCGTCTT CCATTCAAGA 3901 CTCGGGAACA GCCGCCATTT TGCTGCGCTC CCCCCAACCC CCAGTTCAGG GCAACCTTGC 3961 TCGCGGACCC AGACTACAGC CCTTGGCGGT CTCTCCACAC GCTTCCGTCC CACCGAGCGG 4021 CCCGGCGGCC ACGAAAGCCC CGGCCAGCCC AGCAGCCCGC TACTCACCAA GTGACGATCA 4081 CAGCGATCCA CAAACAAGAA CCGCGACCCA AATCCCGGCT GCGACGGAAC TAGCTGTGCC 4141 ACACCCGGCG CGTCCTTATA TAATCATCGG CGTTCACCGC CCCACGGAGA TCCCTCCGCA 4201 GAATCGCCGA GAAGGGACTA CTTTTCCTCG CCTGTTCCGC TCTCTGGAAA GAAAACCAGT 4261 GCCCTAGAGT CACCCAAGTC CCGTCCTAAA ATGTCCTTCT GCTGATACTG GGGTTCTAAG 4321 GCCGAGTCTT ATGAGCAGCG GGCCGCTGTC CTGAGCGTCC GGGCGGAAGG ATCAGGACGC 4381 TCGCTGCGCC CTTCGTCTGA CGTGGCAGCG CTCGCCGTGA GGAGGGGGGC GCCCGCGGGA 4441 GGCGCCAAAA CCCGGCGCGG AAGCGCACTG CCGCTCATCG ATGGTCGCGA GTGGAAGCAG 4501 GATGGTTAAG TTCAGAGGTG CAGTAGACAG CTGCAGGACA Tgagggccta tttcccatga 4561 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga 4621 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt 4681 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa 4741 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc GCAAGCTGAC 4801 CCTGAAGTTC GTGTGCTGTC CGAACTTCAG GGTCAGCTTG CTTTTTTCGA GTTAACGTCA 4861 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 4921 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 4981 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 5041 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 5101 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 5161 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 5221 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 5281 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 5341 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 5401 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 5461 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 5521 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 5581 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 5641 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 5701 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 5761 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 5821 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTAC //