LOCUS       MTK1_008                2448 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConLS"                                                            
FEATURES             Location/Qualifiers
     misc_feature    645..787
                     /label="ConS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(1412..1415)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    788..793
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(join(2299..2448,1..614))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     terminator      complement(1425..1533)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    795..819
                     /label="ConS scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1117..1396
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    626..629
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    619..624
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(2194..2298)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    795..798
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1417..1422)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    834..1113
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(1534..2193)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
       61 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      601 ctgtggataa ccgtagtcgg tctcaccctg aattcgcatc tagatggtag agccacaaac
      661 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
      721 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
      781 gcaataccgt ctcactgaac tggccgataa ttgcagacgA TCGAATTCGA GTTGGCGCGC
      841 CTGTCATTCT AAATCTCTCT TTCAGCCTAA AGCTTTTTCC CCGTATCCCC CCAGGTGTCT
      901 GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG
      961 TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG
     1021 CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG
     1081 GGGGCTTTGG GAGGGGGCTG TCCCCGTGAG CTCCCAGGCG CGCCTGTCAT TCTAAATCTC
     1141 TCTTTCAGCC TAAAGCTTTT TCCCCGTATC CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG
     1201 CGAGAAGCGT TCAGAGGAAA GCGATCCCGT GCCACCTTCC CCGTGCCCGG GCTGTCCCCG
     1261 CACGCTGCCG GCTCGGGGAT GCGGGGGGAG CGCCGGACCG GAGCGGAGCC CCGGGCGGCT
     1321 CGCTGCTGCC CCCTAGCGGG GGAGGGACGT AATTACATCC CTGGGGGCTT TGGGGGGGGG
     1381 CTGTCCCCGT GAGCTCAATA AAAGAGCCCA Caacgtgaga ccagaccaat aaaaaacgcc
     1441 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1501 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1561 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1621 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1681 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1741 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     1801 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1861 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1921 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1981 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     2041 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2101 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2161 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2221 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2281 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtt
//