LOCUS       MTK1_010                2448 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConL2:Connector"                                                  
FEATURES             Location/Qualifiers
     protein_bind    complement(1303..1308)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    685..705
                     /label="Con2 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    674..679
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1003..1282
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(join(2185..2448,1..500))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    512..515
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(1311..1419)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(2080..2184)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    681..684
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1420..2079)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    complement(1298..1301)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    531..673
                     /label="Con2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    505..510
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    720..999
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
       61 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      481 ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga ataaatcgca
      541 gccaagtgag tgaatagatg acgcaccacg gtcagacacg gccacatcgt atctcacagg
      601 agcaagcgcg ataggagcac tcacacatag tacggtgatc cgctgactcc tttgcccaaa
      661 taagacgtga gcccgtctca gatgcacaca ctggcttaag atgacATCGA ATTCGAGTTG
      721 GCGCGCCTGT CATTCTAAAT CTCTCTTTCA GCCTAAAGCT TTTTCCCCGT ATCCCCCCAG
      781 GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT
      841 TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA
      901 CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA
      961 TCCCTGGGGG CTTTGGGAGG GGGCTGTCCC CGTGAGCTCC CAGGCGCGCC TGTCATTCTA
     1021 AATCTCTCTT TCAGCCTAAA GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA
     1081 GAGCAGCGAG AAGCGTTCAG AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG
     1141 TCCCCGCACG CTGCCGGCTC GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG
     1201 GCGGCTCGCT GCTGCCCCCT AGCGGGGGAG GGACGTAATT ACATCCCTGG GGGCTTTGGG
     1261 GGGGGGCTGT CCCCGTGAGC TCAATAAAAG AGCCCACaac gtgagaccag accaataaaa
     1321 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1381 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1441 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1501 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1561 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1621 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
     1681 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1741 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1801 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1861 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1921 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1981 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     2041 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     2101 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2161 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2221 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2281 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2341 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2401 atactgttct tctagtgtag ccgtagttag gccaccactt caagaact
//