LOCUS       MTK1_013                2448 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConL5:Connector"                                                  
FEATURES             Location/Qualifiers
     terminator      complement(1921..2029)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1291..1315
                     /label="Con5 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(1908..1911)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(242..346)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(2030..2448,1..241))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    1115..1120
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1284..1289
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1613..1892
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1141..1283
                     /label="Con5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(347..1110)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    1330..1609
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    1122..1125
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1913..1918)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1291..1294
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
       61 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      121 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      181 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      241 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      301 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
      361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1081 gcgttatccc ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga
     1141 acgcacaagg tcagggcact catgcgacaa tcaactcgat gcatgatccg caccattgtc
     1201 gaggggccag cgtcaatagt gccgatgacc acagacccgg ttaagacata gccgaatgga
     1261 gccgcgccga ccacagaatg atacgtctca aagtgaataa agctccacac agtcgATCGA
     1321 ATTCGAGTTG GCGCGCCTGT CATTCTAAAT CTCTCTTTCA GCCTAAAGCT TTTTCCCCGT
     1381 ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC
     1441 CGTGCCACCT TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG
     1501 GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA
     1561 CGTAATTACA TCCCTGGGGG CTTTGGGAGG GGGCTGTCCC CGTGAGCTCC CAGGCGCGCC
     1621 TGTCATTCTA AATCTCTCTT TCAGCCTAAA GCTTTTTCCC CGTATCCCCC CAGGTGTCTG
     1681 CAGGCTCAAA GAGCAGCGAG AAGCGTTCAG AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT
     1741 GCCCGGGCTG TCCCCGCACG CTGCCGGCTC GGGGATGCGG GGGGAGCGCC GGACCGGAGC
     1801 GGAGCCCCGG GCGGCTCGCT GCTGCCCCCT AGCGGGGGAG GGACGTAATT ACATCCCTGG
     1861 GGGCTTTGGG GGGGGGCTGT CCCCGTGAGC TCAATAAAAG AGCCCACaac gtgagaccag
     1921 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1981 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     2041 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2101 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2161 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2221 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     2281 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2341 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     2401 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagct
//