LOCUS       MTK1_023                2056 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConL1:Connector"                                                  
FEATURES             Location/Qualifiers
     terminator      complement(1188..1296)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1024..1037
                     /label="CTCF"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    950..953
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1297..1956)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     rep_origin      complement(6..769)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    complement(1180..1185)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1175..1178)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    950..974
                     /label="Con1 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(join(1957..2056,1..5))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    943..948
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    975..1174
                     /label="B1 insulator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    800..942
                     /label="Con1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    774..779
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    781..784
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
       61 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca
      781 ccctgaattc gcatctagaa cggggtcatc acggctcatc atgcgccaaa caaatgtgtg
      841 caatacacgc tcggatgact gcatgatgac cgcactgact ggggacagca gatccaccta
      901 agcctgtgag agaagcagac acccgacaga tcaaggcagt tacgtctcac caaaccagat
      961 gtcaacacag ctacggtctg ccgcttcagg tccatttacg gaagccgcaa tcctgcacta
     1021 tgaccaccag ggggagcgct aggcccatcc ctgcacaggt agctattttt agaggttgct
     1081 tctagcctgg aggaagaaac gaaagaaata aaagactatg atactttttt tttttaaccg
     1141 gaccaactta taaattattt gtaagtccaa taaaaacgtg agaccagacc aataaaaaac
     1201 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1261 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1321 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1381 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1441 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1501 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1561 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1621 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1681 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1741 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1801 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1861 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1921 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     1981 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2041 acctcttacg tgcccg
//