LOCUS       MTK1_026                2061 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConL4:Connector"                                                  
FEATURES             Location/Qualifiers
     CDS             complement(1028..1687)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    669..674
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(911..916)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1688..1792)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    507..510
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    700..905
                     /label="E1 insulator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    676..699
                     /label="Con4 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(919..1027)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    526..668
                     /label="Con4"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    676..679
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    500..505
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(join(1793..2061,1..495))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    complement(906..909)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       61 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      481 tctgtggata accgtagtcg gtctcaccct gaattcgcat ctagagcctg cactagacga
      541 actaggcaag atgcgtccaa tccgtctaaa catggtgaca acgctggaca gatgacgtaa
      601 caccgagcca catcctgaaa tcgaggcagg ctaaccgaaa ccgtgacaat gcaaagagac
      661 agcctgaccg tctcaggtag actacccatg agtcacatga aggagtgggc ctggaataaa
      721 accagaaatc cccattctca gtctgcccca agccctccaa ttccgagggc gctgtatgta
      781 taagctcggg caggcaaaag tcgactgtga gaacacgcca gcagagggcg ctgtggcccc
      841 atcagtccct gcccactgaa ctctccagaa ggaaaagcgg caaggatgca aacaagaaaa
      901 tcagaaacgt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      961 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1021 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1081 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1141 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1201 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
     1261 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1321 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1381 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1441 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1501 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1561 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1621 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1681 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1741 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1981 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2041 caccacttca agaactctgt a
//