LOCUS       MTK1_034                1905 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConL5:Connector"                                                  
FEATURES             Location/Qualifiers
     promoter        complement(242..346)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(1487..1905,1..241))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1303..1364
                     /label="P2A Sequence + GG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    1284..1289
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1303..1362
                     /label="part1 from 2nd TU"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1297..1299
                     /label="part1 from 2nd TU"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    1115..1120
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1291..1294
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1122..1125
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(1378..1486)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1141..1283
                     /label="Con5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(1370..1375)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1365..1368)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1291..1294
                     /label="Con5 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1297..1299
                     /label="P2A Sequence + GG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(347..1110)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
       61 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      121 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      181 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      241 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      301 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
      361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1081 gcgttatccc ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga
     1141 acgcacaagg tcagggcact catgcgacaa tcaactcgat gcatgatccg caccattgtc
     1201 gaggggccag cgtcaatagt gccgatgacc acagacccgg ttaagacata gccgaatgga
     1261 gccgcgccga ccacagaatg atacgtctca aagtggggta gtggagccac gaatttcagt
     1321 ttattaaaac aggctggcga tgtcgaagag aaccctgggc caGGaacgtg agaccagacc
     1381 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1441 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1501 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1561 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1621 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1681 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1741 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1801 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1861 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagct
//