LOCUS       MTK2_001                2080 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     CDS             complement(1316..1975)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    769..775
                     /label="BsaI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    878..896
                     /label="tetO"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1199..1204
                     /label="BsaI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1207..1315)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    802..1147
                     /label="tet3g promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(1976..2080)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1..764)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    806..824
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcGG TCTCaAACgG
      781 ccctttcgtc ttcactcgag tttactccct atcagtgata gagaacgtat gaagagttta
      841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
      901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
      961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     1021 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa
     1081 agcagagctc gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct
     1141 tatacttacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg atcTATGtGA
     1201 GACCagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1261 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1321 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1381 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1441 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1501 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1561 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1621 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1681 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1741 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1801 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1861 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1921 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1981 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2041 cattatggtg aaagttggaa cctcttacgt gcccgatcaa
//