LOCUS       MTK2_004                2194 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     promoter        complement(join(2193..2194,1..103))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1277..1282
                     /label="SP1 Binding Site"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1075..1080
                     /label="SP1 Binding Site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1265..1270
                     /label="SP1 Binding Site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1424..1532)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(104..867)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    883..1411
                     /label="pPGK(MTK)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        883..1382
                     /label="PGK promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1416..1421
                     /label="BsaI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1253..1258
                     /label="SP1 Binding Site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1282..1287
                     /label="SP1 Binding Site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    872..878
                     /label="BsaI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(1533..2192)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
       61 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct
      121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      841 ttatcccctg attctgtgga taaccgtagT CGGTCTCaAA CGgggtaggg gaggcgcttt
      901 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
      961 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     1021 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     1081 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc aTgtctcact aggctcgtgc
     1141 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     1201 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     1261 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     1321 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     1381 cgacctgcag cccaagcttg tcgacgccac TATGtGAGAC Cagaccaata aaaaacgccc
     1441 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1501 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1561 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1621 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1681 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1741 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1801 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1861 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1921 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1981 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     2041 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     2101 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     2161 acggtggtat atccagtgat ttttttctcc attt
//