LOCUS       MTK2_005                3336 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     CDS             complement(2572..3231)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(2463..2571)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2455..2460
                     /label="BsaI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2398..2417
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    780..2444
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     regulatory      801..1088
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1352..1371
                     /label="sgRNA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(1..767)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     promoter        complement(3232..3336)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    769..775
                     /label="BsaI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccGTAgtcGG TCTCaAACGC
      781 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA
      841 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA
      901 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC
      961 AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT
     1021 GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT
     1081 TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT
     1141 CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA
     1201 TGGGGGCGGG GGGGGGGGGG GCGCGCGCCA GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC
     1261 GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG AAAGTTTCCT
     1321 TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG GCGGGCGGGA
     1381 GTCGCTGCGT TGCCTTCGCC CCGTGCCCCG CTCCGCGCCG CCTCGCGCCG CCCGCCCCGG
     1441 CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC TCCTCCGGGC
     1501 TGTAATTAGC GCTTGGTTTA ATGACGGCTC GTTTCTTTTC TGTGGCTGCG TGAAAGCCTT
     1561 AAAGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGGAGCGGC TCGGGGGGTG CGTGCGTGTG
     1621 TGTGTGCGTG GGGAGCGCCG CGTGCGGCCC GCGCTGCCCG GCGGCTGTGA GCGCTGCGGG
     1681 CGCGGCGCGG GGCTTTGTGC GCTCCGCGTG TGCGCGAGGG GAGCGCGGCC GGGGGCGGTG
     1741 CCCCGCGGTG CGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG TGTGCGTGGG
     1801 GGGGTGAGCA GGGGGTGTGG GCGCGGCGGT CGGGCTGTAA CCCCCCCCTG CACCCCCCTC
     1861 CCCGAGTTGC TGAGCACGGC CCGGCTTCGG GTGCGGGGCT CCGTaCGGGG CGTGGCGCGG
     1921 GGCTCGCCGT GCCGGGCGGG GGGTGGCGGC AGGTGGGGGT GCCGGGCGGG GCGGGGCCGC
     1981 CTCGGGCCGG GGAGGGCTCG GGGGAGGGGC GCGGCGGcCC CCGGAGcGCC GGCGGCTGTC
     2041 GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC
     2101 TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA
     2161 GCGGGCGCgG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC
     2221 GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC cTCTCCAGCC TCGGGGCTGT CCGCgGGGGG
     2281 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
     2341 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
     2401 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGa attcgccacT ATGtGAGACC
     2461 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     2521 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     2581 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     2641 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     2701 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     2761 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
     2821 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2881 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     2941 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     3001 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     3061 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     3121 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     3181 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     3241 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     3301 atggtgaaag ttggaacctc ttacgtgccc gatcaa
//