LOCUS       MTK2_014                1921 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    597..597
                     /label="g-->c, remove BSmbI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    662..667
                     /label="BsaI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    408..422
                     /label="added after sequencing"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    391..397
                     /label="BsaI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    528..533
                     /label="SpeI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    402..651
                     /label="GAL4-UAS promotor"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        534..651
                     /label="mCMV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(670..778)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    508..527
                     /label="Gal4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    402..487
                     /label="Gal4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(779..1438)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     rep_origin      complement(join(1544..1921,1..389))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     promoter        complement(1439..1543)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccGTAgtc GGTCTCaAAC GggagcaCTG TCCTCCGAAC
      421 GTcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
      481 ccgaacgGAG CATGTCCTCC GAACGTCGGA GCACTGTCCT CCGAACGact agttaggcgt
      541 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctgCaga
      601 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cgccAcTATG
      661 tGAGACCaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
      721 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
      781 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      841 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      901 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      961 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
     1021 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1081 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1141 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1201 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1261 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1321 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1381 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1441 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1501 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 g
//