LOCUS       MTK3_009                4059 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    3334..3363
                     /label="Linker misc_feature"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(497..601)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    1370..1375
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3364..3750
                     /label="VP16 misc_feature"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(join(3896..4059,1..496))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    complement(3779..3784)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            1381..3327
                     /label="PhyB (1-650) gene"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3787..3895)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3751..3771
                     /label="pSAM_rev misc_feature"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(602..1365)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
       61 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      121 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      181 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      241 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
      301 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      361 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      421 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      481 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      541 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      601 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcatatg
     1381 gtttccggag tcgggggtag tggcggtggc cgtggcggtg gccgtggcgg agaagaagaa
     1441 ccgtcgtcaa gtcacactcc taataaccga agaggaggag aacaagctca atcgtcggga
     1501 acgaaatctc tcagaccaag aagcaacact gaatcaatga gcaaagcaat tcaacagtac
     1561 accgtcgacg caagactcca cgccgttttc gaacaatccg gcgaatcagg gaaatcattc
     1621 gactactcac aatcactcaa aacgacgacg tacggttcct ctgtacctga gcaacagatc
     1681 acagcttatc tctctcgaat ccagcgaggt ggttacattc agcctttcgg atgtatgatc
     1741 gccgtcgatg aatccagttt ccggatcatc ggttacagtg aaaacgccag agaaatgtta
     1801 gggattatgc ctcaatctgt tcctactctt gagaaacctg agattctagc tatgggaact
     1861 gatgtgagat ctttgttcac ttcttcgagc tcgattctac tcgagcgtgc tttcgttgct
     1921 cgagagatta ccttgttaaa tccggtttgg atccattcca agaatactgg taaaccgttt
     1981 tacgccattc ttcataggat tgatgttggt gttgttattg atttagagcc agctagaact
     2041 gaagatcctg cgctttctat tgctggtgct gttcaatcgc agaaactcgc ggttcgtgcg
     2101 atttctcagt tacaggctct tcctggtgga gatattaagc ttttgtgtga cactgtcgtg
     2161 gaaagtgtga gggacttgac tggttatgat cgtgttatgg tttataagtt tcatgaagat
     2221 gagcatggag aagttgtagc tgagagtaaa cgagatgatt tagagcctta tattggactg
     2281 cattatcctg ctactgatat tcctcaagcg tcaaggttct tgtttaagca gaaccgtgtc
     2341 cgaatgatag tagattgcaa tgccacacct gttcttgtgg tccaggacga taggctaact
     2401 cagtctatgt gcttggttgg ttctactctt agggctcctc atggttgtca ctctcagtat
     2461 atggctaaca tgggatctat tgcgtcttta gcaatggcgg ttataatcaa tggaaatgaa
     2521 gatgatggga gcaatgtagc tagtggaaga agctcgatga ggctttgggg tttggttgtt
     2581 tgccatcaca cttcttctcg ctgcataccg tttccgctaa ggtatgcttg tgagtttttg
     2641 atgcaggctt tcggtttaca gttaaacatg gaattgcagt tagctttgca aatgtcagag
     2701 aaacgcgttt tgagaacgca gacactgtta tgtgatatgc ttctgcgtga ctcgcctgct
     2761 ggaattgtta cacagagtcc cagtatcatg gacttagtga aatgtgacgg tgcagcattt
     2821 ctttaccacg ggaagtatta cccgttgggt gttgctccta gtgaagttca gataaaagat
     2881 gttgtggagt ggttgcttgc gaatcatgcg gattcaaccg gattaagcac tgatagttta
     2941 ggcgatgcgg ggtatcccgg tgcagctgcg ttaggggatg ctgtgtgcgg tatggcagtt
     3001 gcatatatca caaaaagaga ctttcttttt tggtttcgat ctcacactgc gaaagaaatc
     3061 aaatggggag gcgctaagca tcatccggag gataaagatg atgggcaacg aatgcatcct
     3121 cgttcgtcct ttcaggcttt tcttgaagtt gttaagagcc ggagtcagcc atgggaaact
     3181 gcggaaatgg atgcgattca ctcgctccag cttattctga gagactcttt taaagaatct
     3241 gaggcggcta tgaactctaa agttgtggat ggtgtggttc agccatgtag ggatatggcg
     3301 ggggaacagg ggattgatga gttaggtgaa ttcgatagtg ctggtagtgc tggtagtgct
     3361 ggttccgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat cgagggcctg
     3421 ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc gcgcctgtcc
     3481 tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga tgtcagcctg
     3541 ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac
     3601 gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac
     3661 gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc
     3721 gatgcccttg gaattgacga gtacggtggg cccaagaaaa agcggaaggt gGGATCCtga
     3781 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     3841 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     3901 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     3961 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     4021 gcgtataata tttgcccatg gtgaaaacgg gggcgaaga
//