LOCUS       MTK3_011                2661 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     gene            1446..1745
                     /label="PIF6(1-100) gene"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    769..774
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            780..1409
                     /label="TetR gene"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1746..1772
                     /label="HA-Tag misc_feature"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(1..764)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    complement(1780..1785)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(2557..2661)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1897..2556)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(1788..1896)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcatatgt
      781 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      841 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      901 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      961 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
     1021 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
     1081 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     1141 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     1201 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     1261 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     1321 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
     1381 aatgtgaaag tgggtccgcg tacagccgcg cgctcgatag tgctggtagt gctggtagtg
     1441 ctggtatgat gttcttacca accgattatt gttgcaggtt aagcgatcaa gagtatatgg
     1501 agcttgtgtt tgagaatggc cagattcttg caaagggcca aagatccaac gtttctctgc
     1561 ataatcaacg taccaaatcg atcatggatt tgtatgaggc agagtataac gaggatttca
     1621 tgaagagtat catccatggt ggtggtggtg ccatcacaaa tctcggggac acgcaggttg
     1681 ttccacaaag tcatgttgct gctgcccatg aaacaaacat gttggaaagc aataaacatg
     1741 ttgactaccc atacgatgtt ccagattacg ctGGATCCtg agaccagacc aataaaaaac
     1801 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1861 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1921 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1981 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2041 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2101 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     2161 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2221 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2281 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2341 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2401 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2461 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2521 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2581 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2641 acctcttacg tgcccgatca a
//