LOCUS       MTK3_013                3033 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     promoter        complement(join(2983..3033,1..54))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(55..818)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    complement(2206..2211)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2323..2982)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     feature         834..1271
                     /label="Gal4_DBD"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2214..2322)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1235..1253
                     /label="Gal4_N_term_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1272..2174
                     /label="ABIcs"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     feature         2175..2198
                     /label="FLAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    823..828
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat atgaagctac
      841 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
      901 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
      961 ccaaaaggtc accgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
     1021 tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg
     1081 attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata
     1141 aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta acattgagac
     1201 agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt
     1261 tgactgtatc gACGCGTatg GTGCCTTTGT ATGGTTTTAC TTCGATTTGT GGAAGAAGAC
     1321 CTGAGATGGA AGcTGCTGTT TCGACTATAC CAAGATTCCT TCAATCTTCC TCTGGTTCGA
     1381 TGTTAGATGG TCGGTTTGAT CCTCAATCCG CCGCTCATTT CTTCGGTGTT TACGACGGCC
     1441 ATGGCGGTTC TCAGGTAGCG AACTATTGTA GAGAGAGGAT GCATTTGGCT TTGGCGGAGG
     1501 AGATAGCTAA GGAGAAACCG ATGCTCTGCG ATGGTGATAC GTGGCTGGAG AAGTGGAAGA
     1561 AAGCTCTTTT CAACTCGTTC CTGAGAGTTG ACTCGGAGAT TGAGTCAGTT GCGCCGGAGA
     1621 CaGTTGGGTC AACGTCGGTG GTTGCCGTTG TTTTCCCGTC ACACATCTTC GTCGCTAACT
     1681 GCGGTGACTC TAGAGCCGTT CTTTGCCGCG GCAAAACTGC ACTTCCATTA TCCGTTGACC
     1741 ATAAACCGGA TAGAGAAGAT GAAGCTGCGA GGATTGAAGC CGCAGGAGGG AAAGTGATTC
     1801 AGTGGAATGG AGCTCGTGTT TTCGGTGTTC TCGCCATGTC GAGATCCATT GGCGATAGAT
     1861 ACTTGAAACC ATCCATCATT CCTGATCCGG AAGTGACGGC TGTGAAGAGA GTAAAAGAAG
     1921 ATGATTGTCT GATTTTGGCG AGTGACGGGG TTTGGGATGT AATGACGGAT GAAGAAGCGT
     1981 GTGAGATGGC AAGGAAGCGG ATTCTCTTGT GGCACAAGAA AAACGCGGTG GCTGGGGATG
     2041 CATCGTTGCT CGCGGATGAG CGGAGAAAGG AAGGGAAAGA TCCTGCGGCG ATGTCCGCGG
     2101 CTGAGTATTT GTCAAAGCTG GCGATACAGA GAGGAAGCAA AGACAACATA AGTGTGGTGG
     2161 TGGTTGATTT GAAGGATTAC AAGGACGATG ACGATAAGGG ATCCtgagac cagaccaata
     2221 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     2281 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     2341 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     2401 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2461 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2521 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
     2581 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2641 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     2701 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     2761 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     2821 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2881 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2941 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     3001 tgaaaatctc gataactcaa aaaatacgcc cgg
//