LOCUS       MTK3_018                3537 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    1385..1474
                     /label="hspCas9 (Zhang lab) "
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1793..2734
                     /label="ER-T2"
                     /ApEinfo_revcolor=#ff0f34
                     /ApEinfo_fwdcolor=#ff0f34
     misc_feature    complement(2750..2858)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(join(3519..3537,1..86))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1311..1324
                     /label="BfuAI site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    complement(2742..2747)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1784..1792
                     /label="linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    855..860
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    866..890
                     /label="hspCas9 (Zhang lab) (1-204)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     2091..2110
                     /label="CreERT2-Seq4"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    892..1384
                     /label="hspCas9 (Zhang lab) (1-204)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(87..850)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             complement(2859..3518)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1505..1774
                     /label="FRB"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
ORIGIN
        1 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
       61 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
      121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      301 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      841 ggataaccgt agtcggtctc atatgGACAA GAAGTACAGC ATCGGCCTGG cCATCGGCAC
      901 CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG GTGCCCAGCA AGAAATTCAA
      961 GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC CTGATCGGAG CCCTGCTGTT
     1021 CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA ACCGCCAGAA GAAGATACAC
     1081 CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC AGCAACGAGA TGGCCAAGGT
     1141 GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG GTGGAAGAGG ATAAGAAGCA
     1201 CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG GCCTACCACG AGAAGTACCC
     1261 CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC GACAAGGCCG ACCTGCGGCT
     1321 GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC CACTTCCTGA TCGAGGGCGA
     1381 CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC CAGCTGGTGC AGACCTACAA
     1441 CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGAGGT GGCGGAAGCG GCGGTGGGGG
     1501 AAGCGAGATG TGGCACGAGG GACTCGAAGA GGCCAGCAGG CTGTACTTTG GCGAGAGGAA
     1561 CGTCAAGGGC ATGTTCGAAG TGCTGGAGCC CCTCCATGCG ATGATGGAAA GGGGCCCACA
     1621 GACCCTGAAG GAGACCAGCT TCAACCAGGC TTACGGCAGG GACCTGATGG AGGCACAGGA
     1681 ATGGTGCAGG AAGTACATGA AGAGCGGCAA CGTGAAAGAC CTGACCCAGG CGTGGGACCT
     1741 CTACTACCAC GTGTTCAGGA GGATCAGCAA GCAGggaggt agcctcgagc catctgctgg
     1801 agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa
     1861 cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc
     1921 ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg
     1981 cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt
     2041 gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat gtgcctggct
     2101 agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga agctactgtt
     2161 tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat
     2221 cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga
     2281 gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag
     2341 caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga
     2401 cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct
     2461 ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca
     2521 tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc tggaggcggc
     2581 ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga
     2641 ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa agtattacat
     2701 cacgggggag gcagagggtt tccctgccac agctGGATCC tgagaccaga ccaataaaaa
     2761 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2821 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     2881 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     2941 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     3001 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     3061 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     3121 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     3181 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     3241 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     3301 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     3361 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     3421 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     3481 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcct
//